Restriction map for
pFN21AE1556
(Linear) (Six-base) MAPSORT of: KIEE1556.seq Check: 5713 from: 1 to: 1380
KIEE1556.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 162 256 1380
Size: 130 32 94 1124
Fragments arranged by size:
1124 130 94 32
AflIII A'CryG_T
Cuts at: 0 991 1380
Size: 991 389
AleI CACnn'nnGTG
Cuts at: 0 526 1380
Size: 526 854
AlwNI CAG_nnn'CTG
Cuts at: 0 108 327 812 1132 1191 1380
Size: 108 219 485 320 59 189
Fragments arranged by size:
485 320 219 189 108 59
ApaLI G'TGCA_C
Cuts at: 0 516 1380
Size: 516 864
ApoI r'AATT_y
Cuts at: 0 717 964 972 1142 1173 1380
Size: 717 247 8 170 31 207
Fragments arranged by size:
717 247 207 170 31 8
AvaI C'yCGr_G
Cuts at: 0 852 1380
Size: 852 528
BanII G_rGCy'C
Cuts at: 0 1321 1380
Size: 1321 59
BclI T'GATC_A
Cuts at: 0 99 1380
Size: 99 1281
BglI GCCn_nnn'nGGC
Cuts at: 0 229 1119 1380
Size: 229 890 261
Fragments arranged by size:
890 261 229
BglII A'GATC_T
Cuts at: 0 754 1380
Size: 754 626
Bme1580I G_kGCm'C
Cuts at: 0 520 1380
Size: 520 860
BmrI ACTGGGnnnn_n'
Cuts at: 0 1054 1380
Size: 1054 326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 330 367 879 1380
Size: 217 113 37 512 501
Fragments arranged by size:
512 501 217 113 37
Bpu10I CC'TnA_GC
Cuts at: 0 145 1380
Size: 145 1235
BsaI GGTCTCn'nnnn_
Cuts at: 0 65 163 1261 1380
Size: 65 98 1098 119
Fragments arranged by size:
1098 119 98 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 877 907 1380
Size: 877 30 473
Fragments arranged by size:
877 473 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 832 1289 1380
Size: 832 457 91
Fragments arranged by size:
832 457 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 1380
Size: 630 750
BsiEI CG_ry'CG
Cuts at: 0 56 1380
Size: 56 1324
BsiHKAI G_wGCw'C
Cuts at: 0 425 520 685 1380
Size: 425 95 165 695
Fragments arranged by size:
695 425 165 95
BsmI GAATG_Cn'
Cuts at: 0 620 1380
Size: 620 760
Bsp1286I G_dGCh'C
Cuts at: 0 425 520 685 1321 1380
Size: 425 95 165 636 59
Fragments arranged by size:
636 425 165 95 59
BspHI T'CATG_A
Cuts at: 0 872 1380
Size: 872 508
BsrBI CCG'CTC
Cuts at: 0 33 65 654 1345 1380
Size: 33 32 589 691 35
Fragments arranged by size:
691 589 35 33 32
BsrDI GCAATG_nn'
Cuts at: 0 1029 1380
Size: 1029 351
BstEII G'GTnAC_C
Cuts at: 0 1338 1380
Size: 1338 42
BstYI r'GATC_y
Cuts at: 0 754 1380
Size: 754 626
BtgI C'CryG_G
Cuts at: 0 224 916 1380
Size: 224 692 464
Fragments arranged by size:
692 464 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 624 1380
Size: 624 756
BtsI GCAGTG_nn'
Cuts at: 0 157 1073 1088 1321 1380
Size: 157 916 15 233 59
Fragments arranged by size:
916 233 157 59 15
DraIII CAC_nnn'GTG
Cuts at: 0 515 1168 1380
Size: 515 653 212
Fragments arranged by size:
653 515 212
EaeI y'GGCC_r
Cuts at: 0 53 596 1380
Size: 53 543 784
Fragments arranged by size:
784 543 53
EagI C'GGCC_G
Cuts at: 0 53 1380
Size: 53 1327
EarI CTCTTCn'nnn_
Cuts at: 0 625 686 947 1380
Size: 625 61 261 433
Fragments arranged by size:
625 433 261 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 83 1380
Size: 83 1297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 162 217 256 330 367 879 1380
Size: 130 32 55 39 74 37 512 501
Fragments arranged by size:
512 501 130 74 55 39 37 32
EcoO109I rG'GnC_Cy
Cuts at: 0 231 567 591 1380
Size: 231 336 24 789
Fragments arranged by size:
789 336 231 24
FspI TGC'GCA
Cuts at: 0 301 1380
Size: 301 1079
HaeII r_GCGC'y
Cuts at: 0 224 1380
Size: 224 1156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 653 685 784 816 1380
Size: 653 32 99 32 564
Fragments arranged by size:
653 564 99 32 32
HincII GTy'rAC
Cuts at: 0 372 1380
Size: 372 1008
HpaI GTT'AAC
Cuts at: 0 372 1380
Size: 372 1008
MslI CAynn'nnrTG
Cuts at: 0 415 526 871 1380
Size: 415 111 345 509
Fragments arranged by size:
509 415 345 111
MspA1I CmG'CkG
Cuts at: 0 263 812 1380
Size: 263 549 568
Fragments arranged by size:
568 549 263
NcoI C'CATG_G
Cuts at: 0 224 916 1380
Size: 224 692 464
Fragments arranged by size:
692 464 224
NdeI CA'TA_TG
Cuts at: 0 360 1380
Size: 360 1020
NspI r_CATG'y
Cuts at: 0 318 995 1380
Size: 318 677 385
Fragments arranged by size:
677 385 318
PasI CC'CwG_GG
Cuts at: 0 1116 1380
Size: 1116 264
PciI A'CATG_T
Cuts at: 0 991 1380
Size: 991 389
PflMI CCAn_nnn'nTGG
Cuts at: 0 1309 1380
Size: 1309 71
PpuMI rG'GwC_Cy
Cuts at: 0 591 1380
Size: 591 789
PstI C_TGCA'G
Cuts at: 0 127 332 754 931 1380
Size: 127 205 422 177 449
Fragments arranged by size:
449 422 205 177 127
PvuII CAG'CTG
Cuts at: 0 812 1380
Size: 812 568
SapI GCTCTTCn'nnn_
Cuts at: 0 625 1380
Size: 625 755
SbfI CC_TGCA'GG
Cuts at: 0 332 1380
Size: 332 1048
SfcI C'TryA_G
Cuts at: 0 123 328 750 927 1380
Size: 123 205 422 177 453
Fragments arranged by size:
453 422 205 177 123
StuI AGG'CCT
Cuts at: 0 463 1380
Size: 463 917
StyI C'CwwG_G
Cuts at: 0 203 224 916 1380
Size: 203 21 692 464
Fragments arranged by size:
692 464 203 21
TatI w'GTAC_w
Cuts at: 0 17 491 1062 1380
Size: 17 474 571 318
Fragments arranged by size:
571 474 318 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1313 1345 1380
Size: 1313 32 35
Fragments arranged by size:
1313 35 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 1380
Size: 589 791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 1287 1380
Size: 180 1107 93
Fragments arranged by size:
1107 180 93
XmnI GAAnn'nnTTC
Cuts at: 0 976 1380
Size: 976 404
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI ApoI AvaI BanII
BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrDI BstEII BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII
Hin4I HincII HpaI MslI MspA1I NcoI NdeI NspI
PasI PciI PflMI PpuMI PstI PvuII SapI SbfI
SfcI StuI StyI TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BseYI BsiWI BsmBI BspEI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XhoI XmaI ZraI