Restriction map for
pFN21AE1554
(Linear) (Six-base) MAPSORT of: KIEE1554.seq Check: 1188 from: 1 to: 819
KIEE1554.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
Acc65I G'GTAC_C
Cuts at: 0 721 819
Size: 721 98
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 421 701 765 819
Size: 64 357 280 64 54
Fragments arranged by size:
357 280 64 64 54
AlwNI CAG_nnn'CTG
Cuts at: 0 381 819
Size: 381 438
AseI AT'TA_AT
Cuts at: 0 545 819
Size: 545 274
AvaI C'yCGr_G
Cuts at: 0 477 819
Size: 477 342
BanI G'GyrC_C
Cuts at: 0 104 721 819
Size: 104 617 98
Fragments arranged by size:
617 104 98
BanII G_rGCy'C
Cuts at: 0 484 819
Size: 484 335
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 819
Size: 70 749
BglII A'GATC_T
Cuts at: 0 251 819
Size: 251 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 501 819
Size: 501 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 819
Size: 262 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 819
Size: 289 530
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 758 819
Size: 758 61
BseYI C'CCAG_C
Cuts at: 0 165 631 819
Size: 165 466 188
Fragments arranged by size:
466 188 165
BsiHKAI G_wGCw'C
Cuts at: 0 816 819
Size: 816 3
Bsp1286I G_dGCh'C
Cuts at: 0 484 816 819
Size: 484 332 3
Fragments arranged by size:
484 332 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 647 819
Size: 647 172
BstYI r'GATC_y
Cuts at: 0 20 251 443 819
Size: 20 231 192 376
Fragments arranged by size:
376 231 192 20
BtgI C'CryG_G
Cuts at: 0 97 640 819
Size: 97 543 179
Fragments arranged by size:
543 179 97
EarI CTCTTCn'nnn_
Cuts at: 0 397 819
Size: 397 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 262 421 701 765 819
Size: 64 198 159 280 64 54
Fragments arranged by size:
280 198 159 64 64 54
EcoO109I rG'GnC_Cy
Cuts at: 0 129 819
Size: 129 690
HaeII r_GCGC'y
Cuts at: 0 698 819
Size: 698 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 683 715 819
Size: 37 32 614 32 104
Fragments arranged by size:
614 104 37 32 32
HindIII A'AGCT_T
Cuts at: 0 278 819
Size: 278 541
KpnI G_GTAC'C
Cuts at: 0 725 819
Size: 725 94
MslI CAynn'nnrTG
Cuts at: 0 102 160 819
Size: 102 58 659
Fragments arranged by size:
659 102 58
MspA1I CmG'CkG
Cuts at: 0 556 819
Size: 556 263
NcoI C'CATG_G
Cuts at: 0 97 640 819
Size: 97 543 179
Fragments arranged by size:
543 179 97
NspI r_CATG'y
Cuts at: 0 174 819
Size: 174 645
PpuMI rG'GwC_Cy
Cuts at: 0 129 819
Size: 129 690
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 619 651 819
Size: 619 32 168
Fragments arranged by size:
619 168 32
PvuII CAG'CTG
Cuts at: 0 556 819
Size: 556 263
SmlI C'TyrA_G
Cuts at: 0 304 819
Size: 304 515
SphI G_CATG'C
Cuts at: 0 174 819
Size: 174 645
StyI C'CwwG_G
Cuts at: 0 97 640 819
Size: 97 543 179
Fragments arranged by size:
543 179 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 134 166 819
Size: 134 32 653
Fragments arranged by size:
653 134 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 357 819
Size: 162 195 462
Fragments arranged by size:
462 195 162
Enzymes that do cut:
Acc65I AcuI AlwNI AseI AvaI BanI BanII BbsI
BglII BmrI BpmI BpuEI BseRI BseYI BsiHKAI Bsp1286I
BstXI BstYI BtgI EarI Eco57MI EcoO109I HaeII Hin4I
HindIII KpnI MslI MspA1I NcoI NspI PpuMI PsrI
PvuII SmlI SphI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI