Restriction map for
pFN21AE1552
(Linear) (Six-base) MAPSORT of: KIEE1552.seq Check: 6201 from: 1 to: 1680
KIEE1552.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1519 1680
Size: 1519 161
AccI GT'mk_AC
Cuts at: 0 1616 1680
Size: 1616 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1474 1680
Size: 1159 315 206
Fragments arranged by size:
1159 315 206
AflIII A'CryG_T
Cuts at: 0 686 1680
Size: 686 994
AleI CACnn'nnGTG
Cuts at: 0 1037 1680
Size: 1037 643
AlwNI CAG_nnn'CTG
Cuts at: 0 1137 1392 1680
Size: 1137 255 288
Fragments arranged by size:
1137 288 255
ApoI r'AATT_y
Cuts at: 0 553 1150 1633 1680
Size: 553 597 483 47
Fragments arranged by size:
597 553 483 47
BanI G'GyrC_C
Cuts at: 0 1555 1680
Size: 1555 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1300 1680
Size: 1300 380
BlpI GC'TnA_GC
Cuts at: 0 828 1680
Size: 828 852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1547 1612 1680
Size: 976 571 65 68
Fragments arranged by size:
976 571 68 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1680
Size: 1228 452
BsaAI yAC'GTr
Cuts at: 0 341 1290 1680
Size: 341 949 390
Fragments arranged by size:
949 390 341
BsaBI GATnn'nnATC
Cuts at: 0 146 1680
Size: 146 1534
BsaHI Gr'CG_yC
Cuts at: 0 985 1680
Size: 985 695
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 640 670 1680
Size: 640 30 1010
Fragments arranged by size:
1010 640 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 1680
Size: 655 1025
BseYI C'CCAG_C
Cuts at: 0 433 1064 1352 1583 1680
Size: 433 631 288 231 97
Fragments arranged by size:
631 433 288 231 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1499 1680
Size: 1071 428 181
Fragments arranged by size:
1071 428 181
BsiHKAI G_wGCw'C
Cuts at: 0 280 961 1073 1275 1680
Size: 280 681 112 202 405
Fragments arranged by size:
681 405 280 202 112
Bsp1286I G_dGCh'C
Cuts at: 0 280 961 1073 1275 1680
Size: 280 681 112 202 405
Fragments arranged by size:
681 405 280 202 112
BspHI T'CATG_A
Cuts at: 0 1155 1680
Size: 1155 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 499 1519 1680
Size: 499 1020 161
Fragments arranged by size:
1020 499 161
BsrDI GCAATG_nn'
Cuts at: 0 1237 1680
Size: 1237 443
BstXI CCAn_nnnn'nTGG
Cuts at: 0 718 1039 1348 1680
Size: 718 321 309 332
Fragments arranged by size:
718 332 321 309
BstYI r'GATC_y
Cuts at: 0 511 1680
Size: 511 1169
BtgI C'CryG_G
Cuts at: 0 270 711 976 1680
Size: 270 441 265 704
Fragments arranged by size:
704 441 270 265
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 605 1680
Size: 605 1075
BtsI GCAGTG_nn'
Cuts at: 0 1073 1083 1680
Size: 1073 10 597
Fragments arranged by size:
1073 597 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 339 374 1680
Size: 339 35 1306
Fragments arranged by size:
1306 339 35
DraIII CAC_nnn'GTG
Cuts at: 0 1427 1680
Size: 1427 253
DrdI GACnn_nn'nnGTC
Cuts at: 0 265 1680
Size: 265 1415
EaeI y'GGCC_r
Cuts at: 0 360 1680
Size: 360 1320
EarI CTCTTCn'nnn_
Cuts at: 0 149 1597 1680
Size: 149 1448 83
Fragments arranged by size:
1448 149 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1159 1474 1547 1612 1680
Size: 976 183 315 73 65 68
Fragments arranged by size:
976 315 183 73 68 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 425 670 1680
Size: 425 245 1010
Fragments arranged by size:
1010 425 245
EcoO109I rG'GnC_Cy
Cuts at: 0 430 1134 1316 1379 1531 1587 1680
Size: 430 704 182 63 152 56 93
Fragments arranged by size:
704 430 182 152 93 63 56
HaeII r_GCGC'y
Cuts at: 0 1051 1680
Size: 1051 629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 583 615 1680
Size: 3 32 548 32 1065
Fragments arranged by size:
1065 548 32 32 3
HincII GTy'rAC
Cuts at: 0 630 1680
Size: 630 1050
HindIII A'AGCT_T
Cuts at: 0 880 1680
Size: 880 800
MslI CAynn'nnrTG
Cuts at: 0 691 1018 1037 1202 1680
Size: 691 327 19 165 478
Fragments arranged by size:
691 478 327 165 19
MspA1I CmG'CkG
Cuts at: 0 293 404 437 1680
Size: 293 111 33 1243
Fragments arranged by size:
1243 293 111 33
NcoI C'CATG_G
Cuts at: 0 270 711 976 1680
Size: 270 441 265 704
Fragments arranged by size:
704 441 270 265
NspI r_CATG'y
Cuts at: 0 290 640 690 1680
Size: 290 350 50 990
Fragments arranged by size:
990 350 290 50
PasI CC'CwG_GG
Cuts at: 0 1026 1680
Size: 1026 654
PciI A'CATG_T
Cuts at: 0 686 1680
Size: 686 994
PflMI CCAn_nnn'nTGG
Cuts at: 0 1261 1392 1680
Size: 1261 131 288
Fragments arranged by size:
1261 288 131
PfoI T'CCnGG_A
Cuts at: 0 1391 1680
Size: 1391 289
PpuMI rG'GwC_Cy
Cuts at: 0 430 1134 1316 1379 1680
Size: 430 704 182 63 301
Fragments arranged by size:
704 430 301 182 63
PsiI TTA'TAA
Cuts at: 0 146 1680
Size: 146 1534
PstI C_TGCA'G
Cuts at: 0 739 1680
Size: 739 941
PvuII CAG'CTG
Cuts at: 0 293 437 1680
Size: 293 144 1243
Fragments arranged by size:
1243 293 144
SanDI GG'GwC_CC
Cuts at: 0 1379 1680
Size: 1379 301
SfcI C'TryA_G
Cuts at: 0 735 1680
Size: 735 945
SmlI C'TyrA_G
Cuts at: 0 1243 1680
Size: 1243 437
SphI G_CATG'C
Cuts at: 0 640 1680
Size: 640 1040
StyI C'CwwG_G
Cuts at: 0 270 711 976 1680
Size: 270 441 265 704
Fragments arranged by size:
704 441 270 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 989 1056 1636 1680
Size: 989 67 580 44
Fragments arranged by size:
989 580 67 44
TatI w'GTAC_w
Cuts at: 0 1190 1680
Size: 1190 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 543 575 1680
Size: 543 32 1105
Fragments arranged by size:
1105 543 32
XbaI T'CTAG_A
Cuts at: 0 1275 1680
Size: 1275 405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1039 1522 1680
Size: 1039 483 158
Fragments arranged by size:
1039 483 158
XmnI GAAnn'nnTTC
Cuts at: 0 1151 1183 1680
Size: 1151 32 497
Fragments arranged by size:
1151 497 32
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApoI BanI
BbsI BlpI BpmI BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HindIII MslI MspA1I NcoI NspI PasI PciI PflMI
PfoI PpuMI PsiI PstI PvuII SanDI SfcI SmlI
SphI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI DraI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XhoI XmaI ZraI