Restriction map for
pFN21AE1551
(Linear) (Six-base) MAPSORT of: KIEE1551.seq Check: 6935 from: 1 to: 762
KIEE1551.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 371 762
Size: 371 391
AlwNI CAG_nnn'CTG
Cuts at: 0 649 762
Size: 649 113
ApaI G_GGCC'C
Cuts at: 0 33 758 762
Size: 33 725 4
Fragments arranged by size:
725 33 4
ApoI r'AATT_y
Cuts at: 0 151 762
Size: 151 611
BanII G_rGCy'C
Cuts at: 0 33 58 641 758 762
Size: 33 25 583 117 4
Fragments arranged by size:
583 117 33 25 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 699 762
Size: 77 622 63
Fragments arranged by size:
622 77 63
BbvCI CC'TCA_GC
Cuts at: 0 545 762
Size: 545 217
BclI T'GATC_A
Cuts at: 0 498 762
Size: 498 264
BglI GCCn_nnn'nGGC
Cuts at: 0 686 762
Size: 686 76
BlpI GC'TnA_GC
Cuts at: 0 439 762
Size: 439 323
Bme1580I G_kGCm'C
Cuts at: 0 33 452 703 758 762
Size: 33 419 251 55 4
Fragments arranged by size:
419 251 55 33 4
BmgBI CAC'GTC
Cuts at: 0 90 762
Size: 90 672
BmrI ACTGGGnnnn_n'
Cuts at: 0 379 585 615 762
Size: 379 206 30 147
Fragments arranged by size:
379 206 147 30
Bpu10I CC'TnA_GC
Cuts at: 0 545 762
Size: 545 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 469 762
Size: 469 293
BsaAI yAC'GTr
Cuts at: 0 177 762
Size: 177 585
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 562 762
Size: 523 39 200
Fragments arranged by size:
523 200 39
BseYI C'CCAG_C
Cuts at: 0 24 762
Size: 24 738
BsiHKAI G_wGCw'C
Cuts at: 0 575 641 762
Size: 575 66 121
Fragments arranged by size:
575 121 66
Bsp1286I G_dGCh'C
Cuts at: 0 33 58 452 575 641 703 758 762
Size: 33 25 394 123 66 62 55 4
Fragments arranged by size:
394 123 66 62 55 33 25 4
BsrDI GCAATG_nn'
Cuts at: 0 450 762
Size: 450 312
BssSI C'ACGA_G
Cuts at: 0 682 762
Size: 682 80
Bsu36I CC'TnA_GG
Cuts at: 0 335 762
Size: 335 427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 246 762
Size: 246 516
EaeI y'GGCC_r
Cuts at: 0 135 762
Size: 135 627
EarI CTCTTCn'nnn_
Cuts at: 0 599 762
Size: 599 163
EcoICRI GAG'CTC
Cuts at: 0 639 762
Size: 639 123
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 579 754 755 762
Size: 29 1 549 175 1 7
Fragments arranged by size:
549 175 29 7 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 357 389 762
Size: 357 32 373
Fragments arranged by size:
373 357 32
HincII GTy'rAC
Cuts at: 0 426 762
Size: 426 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 762
Size: 329 433
MscI TGG'CCA
Cuts at: 0 137 762
Size: 137 625
MslI CAynn'nnrTG
Cuts at: 0 49 694 762
Size: 49 645 68
Fragments arranged by size:
645 68 49
MspA1I CmG'CkG
Cuts at: 0 316 762
Size: 316 446
PasI CC'CwG_GG
Cuts at: 0 663 762
Size: 663 99
PshAI GACnn'nnGTC
Cuts at: 0 652 762
Size: 652 110
PspOMI G'GGCC_C
Cuts at: 0 29 754 762
Size: 29 725 8
Fragments arranged by size:
725 29 8
SacI G_AGCT'C
Cuts at: 0 641 762
Size: 641 121
StuI AGG'CCT
Cuts at: 0 722 762
Size: 722 40
StyI C'CwwG_G
Cuts at: 0 750 762
Size: 750 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 762
Size: 160 602
TatI w'GTAC_w
Cuts at: 0 140 762
Size: 140 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 659 691 762
Size: 659 32 71
Fragments arranged by size:
659 71 32
Tth111I GACn'n_nGTC
Cuts at: 0 88 762
Size: 88 674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 762
Size: 154 608
Enzymes that do cut:
AhdI AlwNI ApaI ApoI BanII BbsI BbvCI BclI
BglI BlpI Bme1580I BmgBI BmrI Bpu10I BsaI BsaAI
BseRI BseYI BsiHKAI Bsp1286I BsrDI BssSI Bsu36I BtgZI
EaeI EarI EcoICRI EcoO109I Hin4I HincII MmeI MscI
MslI MspA1I PasI PshAI PspOMI SacI StuI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BfrBI BglII BmtI BplI BpmI BpuEI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmaI XmnI ZraI