Restriction map for
pFN21AE1550
(Linear) (Six-base) MAPSORT of: KIEE1550.seq Check: 6705 from: 1 to: 2541
KIEE1550.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
Acc65I G'GTAC_C
Cuts at: 0 1536 2541
Size: 1536 1005
AclI AA'CG_TT
Cuts at: 0 288 362 2541
Size: 288 74 2179
Fragments arranged by size:
2179 288 74
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 734 1228 1508 2281 2541
Size: 337 397 494 280 773 260
Fragments arranged by size:
773 494 397 337 280 260
AgeI A'CCGG_T
Cuts at: 0 2069 2541
Size: 2069 472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 2541
Size: 200 2341
AlwNI CAG_nnn'CTG
Cuts at: 0 123 303 498 519 763 1707 1807 2541
Size: 123 180 195 21 244 944 100 734
Fragments arranged by size:
944 734 244 195 180 123 100 21
ApaI G_GGCC'C
Cuts at: 0 357 2541
Size: 357 2184
ApaLI G'TGCA_C
Cuts at: 0 1269 2541
Size: 1269 1272
ApoI r'AATT_y
Cuts at: 0 625 640 2541
Size: 625 15 1901
Fragments arranged by size:
1901 625 15
AvaI C'yCGr_G
Cuts at: 0 134 2541
Size: 134 2407
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2131 2164 2541
Size: 2131 33 377
Fragments arranged by size:
2131 377 33
BamHI G'GATC_C
Cuts at: 0 1419 2541
Size: 1419 1122
BanI G'GyrC_C
Cuts at: 0 1536 2541
Size: 1536 1005
BanII G_rGCy'C
Cuts at: 0 357 914 2541
Size: 357 557 1627
Fragments arranged by size:
1627 557 357
BbsI GAAGACnn'nnnn_
Cuts at: 0 325 1741 2541
Size: 325 1416 800
Fragments arranged by size:
1416 800 325
BbvCI CC'TCA_GC
Cuts at: 0 725 1000 2228 2541
Size: 725 275 1228 313
Fragments arranged by size:
1228 725 313 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1603 1637 1984 2018 2541
Size: 1603 34 347 34 523
Fragments arranged by size:
1603 523 347 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 213 1976 2132 2541
Size: 213 1763 156 409
Fragments arranged by size:
1763 409 213 156
BglI GCCn_nnn'nGGC
Cuts at: 0 1630 2541
Size: 1630 911
BglII A'GATC_T
Cuts at: 0 575 2541
Size: 575 1966
Bme1580I G_kGCm'C
Cuts at: 0 357 1273 2541
Size: 357 916 1268
Fragments arranged by size:
1268 916 357
BmgBI CAC'GTC
Cuts at: 0 2209 2541
Size: 2209 332
BmtI G_CTAG'C
Cuts at: 0 374 2541
Size: 374 2167
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1053 2541
Size: 1053 1488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 951 1028 1564 1657 2290 2465 2541
Size: 250 701 77 536 93 633 175 76
Fragments arranged by size:
701 633 536 250 175 93 77 76
Bpu10I CC'TnA_GC
Cuts at: 0 725 913 1000 2228 2234 2541
Size: 725 188 87 1228 6 307
Fragments arranged by size:
1228 725 307 188 87 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2460 2541
Size: 2460 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 2039 2541
Size: 2039 502
BsaWI w'CCGG_w
Cuts at: 0 2069 2103 2135 2180 2541
Size: 2069 34 32 45 361
Fragments arranged by size:
2069 361 45 34 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 784 814 901 931 2541
Size: 784 30 87 30 1610
Fragments arranged by size:
1610 784 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1045 1087 1090 1099 1102 1105 1441 1522
Size: 1045 42 3 9 3 3 336 81
Cuts at: 1522 1525 1528 1531 1534 2541
Size: 3 3 3 3 1007
Fragments arranged by size:
1045 1007 336 81 42 9 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 374 1606 1652 1886 1968 2365 2541
Size: 374 1232 46 234 82 397 176
Fragments arranged by size:
1232 397 374 234 176 82 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1343 2541
Size: 1343 1198
BsiHKAI G_wGCw'C
Cuts at: 0 344 914 1273 2502 2541
Size: 344 570 359 1229 39
Fragments arranged by size:
1229 570 359 344 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 2541
Size: 55 2486
Bsp1286I G_dGCh'C
Cuts at: 0 344 357 914 1273 2502 2541
Size: 344 13 557 359 1229 39
Fragments arranged by size:
1229 557 359 344 39 13
BspEI T'CCGG_A
Cuts at: 0 2103 2541
Size: 2103 438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 2541
Size: 168 2373
BsrDI GCAATG_nn'
Cuts at: 0 1232 2541
Size: 1232 1309
BsrFI r'CCGG_y
Cuts at: 0 1670 2069 2435 2541
Size: 1670 399 366 106
Fragments arranged by size:
1670 399 366 106
BssSI C'ACGA_G
Cuts at: 0 482 2541
Size: 482 2059
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1707 2541
Size: 1707 834
BstEII G'GTnAC_C
Cuts at: 0 67 730 1282 2541
Size: 67 663 552 1259
Fragments arranged by size:
1259 663 552 67
BstXI CCAn_nnnn'nTGG
Cuts at: 0 170 2541
Size: 170 2371
BstYI r'GATC_y
Cuts at: 0 506 575 1215 1419 2541
Size: 506 69 640 204 1122
Fragments arranged by size:
1122 640 506 204 69
Bsu36I CC'TnA_GG
Cuts at: 0 304 1775 2541
Size: 304 1471 766
Fragments arranged by size:
1471 766 304
BtgI C'CryG_G
Cuts at: 0 447 1899 1917 2297 2541
Size: 447 1452 18 380 244
Fragments arranged by size:
1452 447 380 244 18
BtsI GCAGTG_nn'
Cuts at: 0 1528 1875 2541
Size: 1528 347 666
Fragments arranged by size:
1528 666 347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1800 1835 2541
Size: 1800 35 706
Fragments arranged by size:
1800 706 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1454 2541
Size: 1454 1087
EaeI y'GGCC_r
Cuts at: 0 956 2437 2541
Size: 956 1481 104
Fragments arranged by size:
1481 956 104
EarI CTCTTCn'nnn_
Cuts at: 0 827 1076 1144 1874 2031 2257 2541
Size: 827 249 68 730 157 226 284
Fragments arranged by size:
827 730 284 249 226 157 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 1156 1689 2541
Size: 514 642 533 852
Fragments arranged by size:
852 642 533 514
EcoICRI GAG'CTC
Cuts at: 0 912 2541
Size: 912 1629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 337 734 951 1028 1228 1508 1564
Size: 250 87 397 217 77 200 280 56
Cuts at: 1564 1657 2281 2290 2465 2541
Size: 93 624 9 175 76
Fragments arranged by size:
624 397 280 250 217 200 175 93
87 77 76 56 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 802 2541
Size: 307 495 1739
Fragments arranged by size:
1739 495 307
EcoO109I rG'GnC_Cy
Cuts at: 0 300 353 354 1632 1965 2541
Size: 300 53 1 1278 333 576
Fragments arranged by size:
1278 576 333 300 53 1
EcoRV GAT'ATC
Cuts at: 0 1362 2541
Size: 1362 1179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1617 2541
Size: 1617 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 784 816 901 933 1384 1416
Size: 376 32 376 32 85 32 451 32
Cuts at: 1416 1732 1734 1764 1766 1828 1860 2541
Size: 316 2 30 2 62 32 681
Fragments arranged by size:
681 451 376 376 316 85 62 32
32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 0 618 792 2541
Size: 618 174 1749
Fragments arranged by size:
1749 618 174
KpnI G_GTAC'C
Cuts at: 0 1540 2541
Size: 1540 1001
MscI TGG'CCA
Cuts at: 0 958 2541
Size: 958 1583
MslI CAynn'nnrTG
Cuts at: 0 871 892 2541
Size: 871 21 1649
Fragments arranged by size:
1649 871 21
MspA1I CmG'CkG
Cuts at: 0 519 1045 1191 1656 1707 2343 2443 2541
Size: 519 526 146 465 51 636 100 98
Fragments arranged by size:
636 526 519 465 146 100 98 51
NaeI GCC'GGC
Cuts at: 0 2437 2541
Size: 2437 104
NgoMIV G'CCGG_C
Cuts at: 0 2435 2541
Size: 2435 106
NheI G'CTAG_C
Cuts at: 0 370 2541
Size: 370 2171
NspI r_CATG'y
Cuts at: 0 1260 2541
Size: 1260 1281
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1137 1583 2541
Size: 1113 24 446 958
Fragments arranged by size:
1113 958 446 24
PfoI T'CCnGG_A
Cuts at: 0 266 2541
Size: 266 2275
PpuMI rG'GwC_Cy
Cuts at: 0 1965 2541
Size: 1965 576
PspOMI G'GGCC_C
Cuts at: 0 353 2541
Size: 353 2188
PstI C_TGCA'G
Cuts at: 0 128 179 573 1565 2541
Size: 128 51 394 992 976
Fragments arranged by size:
992 976 394 128 51
PvuII CAG'CTG
Cuts at: 0 519 1045 1656 1707 2343 2443 2541
Size: 519 526 611 51 636 100 98
Fragments arranged by size:
636 611 526 519 100 98 51
SacI G_AGCT'C
Cuts at: 0 914 2541
Size: 914 1627
ScaI AGT'ACT
Cuts at: 0 1559 1863 2541
Size: 1559 304 678
Fragments arranged by size:
1559 678 304
SexAI A'CCwGG_T
Cuts at: 0 227 2541
Size: 227 2314
SfcI C'TryA_G
Cuts at: 0 124 175 569 1561 2541
Size: 124 51 394 992 980
Fragments arranged by size:
992 980 394 124 51
SmlI C'TyrA_G
Cuts at: 0 2475 2541
Size: 2475 66
StuI AGG'CCT
Cuts at: 0 2000 2541
Size: 2000 541
StyI C'CwwG_G
Cuts at: 0 591 803 1112 1351 2541
Size: 591 212 309 239 1190
Fragments arranged by size:
1190 591 309 239 212
TatI w'GTAC_w
Cuts at: 0 1557 1861 2541
Size: 1557 304 680
Fragments arranged by size:
1557 680 304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 213 816 2220 2415 2541
Size: 213 603 1404 195 126
Fragments arranged by size:
1404 603 213 195 126
Enzymes that do cut:
Acc65I AclI AcuI AgeI AlfI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BglI BglII Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI Hin4I HincII KpnI MscI MslI
MspA1I NaeI NgoMIV NheI NspI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI ScaI SexAI SfcI SmlI
StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AhdI AleI
AloI AscI AseI AsiSI AvrII BbeI BclI BfrBI
BlpI BmrI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI
BspHI BsrBI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
DraI DraIII EagI EcoRI FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI MmeI NarI NcoI
NdeI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI