Restriction map for
pFN21AE1546
(Linear) (Six-base) MAPSORT of: KIEE1546.seq Check: 5333 from: 1 to: 684
KIEE1546.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 684
Size: 621 63
ApoI r'AATT_y
Cuts at: 0 574 684
Size: 574 110
BanI G'GyrC_C
Cuts at: 0 16 199 684
Size: 16 183 485
Fragments arranged by size:
485 183 16
BanII G_rGCy'C
Cuts at: 0 413 684
Size: 413 271
BbeI G_GCGC'C
Cuts at: 0 203 684
Size: 203 481
BbsI GAAGACnn'nnnn_
Cuts at: 0 555 684
Size: 555 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 684
Size: 249 435
BsaHI Gr'CG_yC
Cuts at: 0 200 684
Size: 200 484
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 241 271 684
Size: 86 30 125 30 413
Fragments arranged by size:
413 125 86 30 30
BseYI C'CCAG_C
Cuts at: 0 264 413 466 684
Size: 264 149 53 218
Fragments arranged by size:
264 218 149 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 684
Size: 265 419
Bsp1286I G_dGCh'C
Cuts at: 0 413 684
Size: 413 271
BspHI T'CATG_A
Cuts at: 0 206 617 684
Size: 206 411 67
Fragments arranged by size:
411 206 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 684
Size: 44 640
BsrDI GCAATG_nn'
Cuts at: 0 605 684
Size: 605 79
BsrFI r'CCGG_y
Cuts at: 0 3 196 684
Size: 3 193 488
Fragments arranged by size:
488 193 3
BstAPI GCAn_nnn'nTGC
Cuts at: 0 23 684
Size: 23 661
BtgI C'CryG_G
Cuts at: 0 611 684
Size: 611 73
BtsI GCAGTG_nn'
Cuts at: 0 599 684
Size: 599 85
DraIII CAC_nnn'GTG
Cuts at: 0 23 684
Size: 23 661
EaeI y'GGCC_r
Cuts at: 0 317 684
Size: 317 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 684
Size: 621 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 684
Size: 147 537
HaeII r_GCGC'y
Cuts at: 0 203 684
Size: 203 481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 684
Size: 86 32 566
Fragments arranged by size:
566 86 32
KasI G'GCGC_C
Cuts at: 0 199 684
Size: 199 485
MslI CAynn'nnrTG
Cuts at: 0 341 610 684
Size: 341 269 74
Fragments arranged by size:
341 269 74
MspA1I CmG'CkG
Cuts at: 0 417 684
Size: 417 267
NaeI GCC'GGC
Cuts at: 0 198 684
Size: 198 486
NarI GG'CG_CC
Cuts at: 0 200 684
Size: 200 484
NgoMIV G'CCGG_C
Cuts at: 0 196 684
Size: 196 488
NspI r_CATG'y
Cuts at: 0 264 684
Size: 264 420
PfoI T'CCnGG_A
Cuts at: 0 362 684
Size: 362 322
SfoI GGC'GCC
Cuts at: 0 201 684
Size: 201 483
SphI G_CATG'C
Cuts at: 0 264 684
Size: 264 420
StyI C'CwwG_G
Cuts at: 0 401 684
Size: 401 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 684
Size: 241 32 411
Fragments arranged by size:
411 241 32
Enzymes that do cut:
AcuI ApoI BanI BanII BbeI BbsI BmrI BsaHI
BsaXI BseYI BsgI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BtgI BtsI DraIII EaeI Eco57MI FalI HaeII
Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI
PfoI SfoI SphI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI