Restriction map for
pFN21AE1544
(Linear) (Six-base) MAPSORT of: KIEE1544.seq Check: 2279 from: 1 to: 1350
KIEE1544.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1350
Size: 1177 173
AflII C'TTAA_G
Cuts at: 0 961 1350
Size: 961 389
AflIII A'CryG_T
Cuts at: 0 732 1350
Size: 732 618
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 1350
Size: 500 32 818
Fragments arranged by size:
818 500 32
AlwNI CAG_nnn'CTG
Cuts at: 0 158 1350
Size: 158 1192
ApaI G_GGCC'C
Cuts at: 0 586 1278 1350
Size: 586 692 72
Fragments arranged by size:
692 586 72
ApoI r'AATT_y
Cuts at: 0 91 664 1350
Size: 91 573 686
Fragments arranged by size:
686 573 91
BanII G_rGCy'C
Cuts at: 0 586 832 1278 1350
Size: 586 246 446 72
Fragments arranged by size:
586 446 246 72
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 926 1165 1350
Size: 317 609 239 185
Fragments arranged by size:
609 317 239 185
BclI T'GATC_A
Cuts at: 0 372 1350
Size: 372 978
Bme1580I G_kGCm'C
Cuts at: 0 152 586 1278 1350
Size: 152 434 692 72
Fragments arranged by size:
692 434 152 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 1281 1350
Size: 1281 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 733 1350
Size: 733 617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 510 592 1350
Size: 479 31 82 758
Fragments arranged by size:
758 479 82 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 808 1350
Size: 61 747 542
Fragments arranged by size:
747 542 61
BsaAI yAC'GTr
Cuts at: 0 974 1350
Size: 974 376
BsaHI Gr'CG_yC
Cuts at: 0 676 1350
Size: 676 674
BsaWI w'CCGG_w
Cuts at: 0 436 1350
Size: 436 914
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 500 530 958 988 1350
Size: 452 30 18 30 428 30 362
Fragments arranged by size:
452 428 362 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 1350
Size: 247 1103
BseYI C'CCAG_C
Cuts at: 0 579 1350
Size: 579 771
BsiHKAI G_wGCw'C
Cuts at: 0 446 832 1350
Size: 446 386 518
Fragments arranged by size:
518 446 386
BsmI GAATG_Cn'
Cuts at: 0 220 1350
Size: 220 1130
Bsp1286I G_dGCh'C
Cuts at: 0 152 446 586 832 1278 1350
Size: 152 294 140 246 446 72
Fragments arranged by size:
446 294 246 152 140 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 788 1350
Size: 788 562
BsrFI r'CCGG_y
Cuts at: 0 67 1350
Size: 67 1283
BstXI CCAn_nnnn'nTGG
Cuts at: 0 720 954 1350
Size: 720 234 396
Fragments arranged by size:
720 396 234
BstYI r'GATC_y
Cuts at: 0 957 1350
Size: 957 393
Bsu36I CC'TnA_GG
Cuts at: 0 259 1350
Size: 259 1091
BtsI GCAGTG_nn'
Cuts at: 0 102 165 473 988 1350
Size: 102 63 308 515 362
Fragments arranged by size:
515 362 308 102 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 1350
Size: 520 830
EaeI y'GGCC_r
Cuts at: 0 536 1220 1350
Size: 536 684 130
Fragments arranged by size:
684 536 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 565 1350
Size: 565 785
EcoICRI GAG'CTC
Cuts at: 0 830 1350
Size: 830 520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 510 592 1177 1350
Size: 479 31 82 585 173
Fragments arranged by size:
585 479 173 82 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 395 1350
Size: 395 955
EcoO109I rG'GnC_Cy
Cuts at: 0 583 800 1275 1350
Size: 583 217 475 75
Fragments arranged by size:
583 475 217 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1152 1184 1350
Size: 1152 32 166
Fragments arranged by size:
1152 166 32
HincII GTy'rAC
Cuts at: 0 126 1350
Size: 126 1224
HindIII A'AGCT_T
Cuts at: 0 949 1350
Size: 949 401
HpaI GTT'AAC
Cuts at: 0 126 1350
Size: 126 1224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1350
Size: 1034 316
MscI TGG'CCA
Cuts at: 0 538 1222 1350
Size: 538 684 128
Fragments arranged by size:
684 538 128
MslI CAynn'nnrTG
Cuts at: 0 718 1350
Size: 718 632
MspA1I CmG'CkG
Cuts at: 0 579 1026 1350
Size: 579 447 324
Fragments arranged by size:
579 447 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 1229 1350
Size: 1229 121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 500 532 1350
Size: 500 32 818
Fragments arranged by size:
818 500 32
PpuMI rG'GwC_Cy
Cuts at: 0 800 1350
Size: 800 550
PspOMI G'GGCC_C
Cuts at: 0 582 1274 1350
Size: 582 692 76
Fragments arranged by size:
692 582 76
PvuII CAG'CTG
Cuts at: 0 579 1026 1350
Size: 579 447 324
Fragments arranged by size:
579 447 324
SacI G_AGCT'C
Cuts at: 0 832 1350
Size: 832 518
SmlI C'TyrA_G
Cuts at: 0 40 823 961 1350
Size: 40 783 138 389
Fragments arranged by size:
783 389 138 40
StyI C'CwwG_G
Cuts at: 0 586 1350
Size: 586 764
TatI w'GTAC_w
Cuts at: 0 1280 1350
Size: 1280 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 958 990 1350
Size: 452 32 474 32 360
Fragments arranged by size:
474 452 360 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 334 940 1350
Size: 334 606 410
Fragments arranged by size:
606 410 334
XmnI GAAnn'nnTTC
Cuts at: 0 314 1350
Size: 314 1036
Enzymes that do cut:
AcuI AflII AflIII AloI AlwNI ApaI ApoI BanII
BbsI BclI Bme1580I BmrI BplI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BstXI BstYI Bsu36I BtsI DrdI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I PflMI PpiI PpuMI
PspOMI PvuII SacI SmlI StyI TatI TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII EagI EarI EcoRI
EcoRV FalI FseI FspI FspAI HaeII KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII XbaI XcmI
XhoI XmaI ZraI