(Linear) (Six-base) MAPSORT of: KIEE1536.seq  Check: 8070  from: 1  to: 969



KIEE1536.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AclI AA'CG_TT

Cuts at:      0     264     969 
   Size:        264     705
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     821     969 
   Size:        821     148
 
AlwNI CAG_nnn'CTG

Cuts at:      0     429     969 
   Size:        429     540
 
ApoI r'AATT_y

Cuts at:      0      85     196     349     969 
   Size:         85     111     153     620

  Fragments arranged by size: 

                 620     153     111      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0     667     969 
   Size:        667     302
 
BglII A'GATC_T

Cuts at:      0     229     796     969 
   Size:        229     567     173

  Fragments arranged by size: 

                 567     229     173
 
BlpI GC'TnA_GC

Cuts at:      0     848     969 
   Size:        848     121
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     648     969 
   Size:         24     624     321

  Fragments arranged by size: 

                 624     321      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     969 
   Size:        163     806
 
BsaHI Gr'CG_yC

Cuts at:      0      74     969 
   Size:         74     895
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     943     969 
   Size:         61     882      26

  Fragments arranged by size: 

                 882      61      26
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     912     969 
   Size:        912      57
 
BspHI T'CATG_A

Cuts at:      0     150     969 
   Size:        150     819
 
BsrDI GCAATG_nn'

Cuts at:      0     227     969 
   Size:        227     742
 
BstYI r'GATC_y

Cuts at:      0     229     796     969 
   Size:        229     567     173

  Fragments arranged by size: 

                 567     229     173
 
BtgI C'CryG_G

Cuts at:      0     101     969 
   Size:        101     868
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     849     969 
   Size:        849     120
 
EaeI y'GGCC_r

Cuts at:      0     104     969 
   Size:        104     865
 
EarI CTCTTCn'nnn_

Cuts at:      0     871     969 
   Size:        871      98
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     648     821     969 
   Size:         24     624     173     148

  Fragments arranged by size: 

                 624     173     148      24
 
EcoRI G'AATT_C

Cuts at:      0     196     349     969 
   Size:        196     153     620

  Fragments arranged by size: 

                 620     196     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     516     548     892     924     969 
   Size:        516      32     344      32      45

  Fragments arranged by size: 

                 516     344      45      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     809     969 
   Size:        357     452     160

  Fragments arranged by size: 

                 452     357     160
 
MscI TGG'CCA

Cuts at:      0     106     969 
   Size:        106     863
 
MspA1I CmG'CkG

Cuts at:      0      54     571     969 
   Size:         54     517     398

  Fragments arranged by size: 

                 517     398      54
 
PfoI T'CCnGG_A

Cuts at:      0     386     969 
   Size:        386     583
 
PvuII CAG'CTG

Cuts at:      0      54     571     969 
   Size:         54     517     398

  Fragments arranged by size: 

                 517     398      54
 
SexAI A'CCwGG_T

Cuts at:      0     953     969 
   Size:        953      16
 
SmlI C'TyrA_G

Cuts at:      0     142     969 
   Size:        142     827
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     807     969 
   Size:        807     162
 
XmnI GAAnn'nnTTC

Cuts at:      0     443     969 
   Size:        443     526
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     BglI    BglII     BlpI     BpmI
    BpuEI    BsaHI    BseRI    BsmBI    BspHI    BsrDI    BstYI     BtgI
    BtgZI     EaeI     EarI  Eco57MI    EcoRI    Hin4I     MmeI     MscI
   MspA1I     PfoI    PvuII    SexAI     SmlI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI