Restriction map for
pFN21AE1525
(Linear) (Six-base) MAPSORT of: KIEE1525.seq Check: 8209 from: 1 to: 771
KIEE1525.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 328 711 771
Size: 309 19 383 60
Fragments arranged by size:
383 309 60 19
AflIII A'CryG_T
Cuts at: 0 148 771
Size: 148 623
AhdI GACnn_n'nnGTC
Cuts at: 0 555 771
Size: 555 216
AlwNI CAG_nnn'CTG
Cuts at: 0 332 771
Size: 332 439
ApoI r'AATT_y
Cuts at: 0 222 250 771
Size: 222 28 521
Fragments arranged by size:
521 222 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 295 328 771
Size: 295 33 443
Fragments arranged by size:
443 295 33
BanI G'GyrC_C
Cuts at: 0 392 771
Size: 392 379
BanII G_rGCy'C
Cuts at: 0 566 771
Size: 566 205
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 315 512 771
Size: 263 52 197 259
Fragments arranged by size:
263 259 197 52
BbvCI CC'TCA_GC
Cuts at: 0 356 771
Size: 356 415
BmrI ACTGGGnnnn_n'
Cuts at: 0 420 771
Size: 420 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 771
Size: 41 730
Bpu10I CC'TnA_GC
Cuts at: 0 356 749 771
Size: 356 393 22
Fragments arranged by size:
393 356 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 771
Size: 439 332
BsaI GGTCTCn'nnnn_
Cuts at: 0 99 771
Size: 99 672
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 466 496 771
Size: 122 30 314 30 275
Fragments arranged by size:
314 275 122 30 30
BsmI GAATG_Cn'
Cuts at: 0 249 771
Size: 249 522
Bsp1286I G_dGCh'C
Cuts at: 0 566 771
Size: 566 205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 675 771
Size: 675 96
BsrGI T'GTAC_A
Cuts at: 0 419 771
Size: 419 352
Bsu36I CC'TnA_GG
Cuts at: 0 89 388 771
Size: 89 299 383
Fragments arranged by size:
383 299 89
BtgI C'CryG_G
Cuts at: 0 11 109 396 771
Size: 11 98 287 375
Fragments arranged by size:
375 287 98 11
DraI TTT'AAA
Cuts at: 0 126 771
Size: 126 645
EarI CTCTTCn'nnn_
Cuts at: 0 209 388 771
Size: 209 179 383
Fragments arranged by size:
383 209 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 309 328 711 771
Size: 41 268 19 383 60
Fragments arranged by size:
383 268 60 41 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 114 771
Size: 114 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 653 685 771
Size: 541 32 80 32 86
Fragments arranged by size:
541 86 80 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 771
Size: 382 389
MspA1I CmG'CkG
Cuts at: 0 546 771
Size: 546 225
NcoI C'CATG_G
Cuts at: 0 11 109 396 771
Size: 11 98 287 375
Fragments arranged by size:
375 287 98 11
NspI r_CATG'y
Cuts at: 0 152 771
Size: 152 619
PciI A'CATG_T
Cuts at: 0 148 771
Size: 148 623
PflMI CCAn_nnn'nTGG
Cuts at: 0 188 771
Size: 188 583
PsiI TTA'TAA
Cuts at: 0 437 771
Size: 437 334
PvuII CAG'CTG
Cuts at: 0 546 771
Size: 546 225
ScaI AGT'ACT
Cuts at: 0 460 604 771
Size: 460 144 167
Fragments arranged by size:
460 167 144
SmlI C'TyrA_G
Cuts at: 0 454 771
Size: 454 317
SspI AAT'ATT
Cuts at: 0 122 237 771
Size: 122 115 534
Fragments arranged by size:
534 122 115
StuI AGG'CCT
Cuts at: 0 349 771
Size: 349 422
StyI C'CwwG_G
Cuts at: 0 11 109 396 771
Size: 11 98 287 375
Fragments arranged by size:
375 287 98 11
TatI w'GTAC_w
Cuts at: 0 419 458 602 771
Size: 419 39 144 169
Fragments arranged by size:
419 169 144 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 466 498 771
Size: 122 32 312 32 273
Fragments arranged by size:
312 273 122 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 163 724 771
Size: 163 561 47
Fragments arranged by size:
561 163 47
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApoI BaeI BanI BanII
BbsI BbvCI BmrI BpmI Bpu10I BpuEI BsaI BsaXI
BsmI Bsp1286I BspMI BsrGI Bsu36I BtgI DraI EarI
Eco57MI FalI Hin4I MmeI MspA1I NcoI NspI PciI
PflMI PsiI PvuII ScaI SmlI SspI StuI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI