Restriction map for
pFN21AE1522
(Linear) (Six-base) MAPSORT of: KIEE1522.seq Check: 1402 from: 1 to: 1776
KIEE1522.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 802 1776
Size: 802 974
AccI GT'mk_AC
Cuts at: 0 1184 1353 1643 1776
Size: 1184 169 290 133
Fragments arranged by size:
1184 290 169 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1616 1776
Size: 1078 538 160
Fragments arranged by size:
1078 538 160
AflIII A'CryG_T
Cuts at: 0 189 798 1776
Size: 189 609 978
Fragments arranged by size:
978 609 189
AlwNI CAG_nnn'CTG
Cuts at: 0 784 817 1237 1776
Size: 784 33 420 539
Fragments arranged by size:
784 539 420 33
ApoI r'AATT_y
Cuts at: 0 55 827 1776
Size: 55 772 949
Fragments arranged by size:
949 772 55
AvaI C'yCGr_G
Cuts at: 0 419 1776
Size: 419 1357
BanII G_rGCy'C
Cuts at: 0 481 702 1776
Size: 481 221 1074
Fragments arranged by size:
1074 481 221
BbsI GAAGACnn'nnnn_
Cuts at: 0 929 1141 1776
Size: 929 212 635
Fragments arranged by size:
929 635 212
BglI GCCn_nnn'nGGC
Cuts at: 0 529 1776
Size: 529 1247
BmgBI CAC'GTC
Cuts at: 0 190 1776
Size: 190 1586
BmrI ACTGGGnnnn_n'
Cuts at: 0 1741 1776
Size: 1741 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1776
Size: 320 1456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 742 1776
Size: 636 106 1034
Fragments arranged by size:
1034 636 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 1241 1776
Size: 1241 535
BsaBI GATnn'nnATC
Cuts at: 0 278 1776
Size: 278 1498
BsaHI Gr'CG_yC
Cuts at: 0 513 1776
Size: 513 1263
BsaWI w'CCGG_w
Cuts at: 0 100 1687 1776
Size: 100 1587 89
Fragments arranged by size:
1587 100 89
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 1776
Size: 367 1409
BseYI C'CCAG_C
Cuts at: 0 650 807 1776
Size: 650 157 969
Fragments arranged by size:
969 650 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1344 1776
Size: 1344 432
BsiHKAI G_wGCw'C
Cuts at: 0 481 1776
Size: 481 1295
BsmI GAATG_Cn'
Cuts at: 0 532 1776
Size: 532 1244
Bsp1286I G_dGCh'C
Cuts at: 0 481 702 1776
Size: 481 221 1074
Fragments arranged by size:
1074 481 221
BspEI T'CCGG_A
Cuts at: 0 100 1776
Size: 100 1676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 802 1308 1776
Size: 802 506 468
Fragments arranged by size:
802 506 468
BsrBI CCG'CTC
Cuts at: 0 76 1776
Size: 76 1700
BsrDI GCAATG_nn'
Cuts at: 0 1209 1265 1673 1776
Size: 1209 56 408 103
Fragments arranged by size:
1209 408 103 56
BsrFI r'CCGG_y
Cuts at: 0 43 1776
Size: 43 1733
BsrGI T'GTAC_A
Cuts at: 0 656 1776
Size: 656 1120
BstAPI GCAn_nnn'nTGC
Cuts at: 0 217 817 1776
Size: 217 600 959
Fragments arranged by size:
959 600 217
BstBI TT'CG_AA
Cuts at: 0 1134 1776
Size: 1134 642
BstZ17I GTA'TAC
Cuts at: 0 1354 1776
Size: 1354 422
BtgI C'CryG_G
Cuts at: 0 464 1776
Size: 464 1312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 737 1776
Size: 737 1039
BtsI GCAGTG_nn'
Cuts at: 0 661 1356 1776
Size: 661 695 420
Fragments arranged by size:
695 661 420
ClaI AT'CG_AT
Cuts at: 0 1472 1776
Size: 1472 304
DraI TTT'AAA
Cuts at: 0 1434 1776
Size: 1434 342
DraIII CAC_nnn'GTG
Cuts at: 0 838 1776
Size: 838 938
EaeI y'GGCC_r
Cuts at: 0 9 263 1776
Size: 9 254 1513
Fragments arranged by size:
1513 254 9
EarI CTCTTCn'nnn_
Cuts at: 0 1159 1441 1771 1776
Size: 1159 282 330 5
Fragments arranged by size:
1159 330 282 5
EcoICRI GAG'CTC
Cuts at: 0 479 1776
Size: 479 1297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1078 1616 1776
Size: 320 758 538 160
Fragments arranged by size:
758 538 320 160
EcoRI G'AATT_C
Cuts at: 0 827 1776
Size: 827 949
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 919 951 1238 1270 1442 1474 1776
Size: 919 32 287 32 172 32 302
Fragments arranged by size:
919 302 287 172 32 32 32
HincII GTy'rAC
Cuts at: 0 161 1185 1776
Size: 161 1024 591
Fragments arranged by size:
1024 591 161
HindIII A'AGCT_T
Cuts at: 0 673 1776
Size: 673 1103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1776
Size: 804 972
MscI TGG'CCA
Cuts at: 0 265 1776
Size: 265 1511
MslI CAynn'nnrTG
Cuts at: 0 469 1776
Size: 469 1307
MspA1I CmG'CkG
Cuts at: 0 654 744 784 901 1776
Size: 654 90 40 117 875
Fragments arranged by size:
875 654 117 90 40
NdeI CA'TA_TG
Cuts at: 0 906 1776
Size: 906 870
NspI r_CATG'y
Cuts at: 0 802 1776
Size: 802 974
PciI A'CATG_T
Cuts at: 0 798 1776
Size: 798 978
PshAI GACnn'nnGTC
Cuts at: 0 1344 1776
Size: 1344 432
PsiI TTA'TAA
Cuts at: 0 286 712 1776
Size: 286 426 1064
Fragments arranged by size:
1064 426 286
PspXI vC'TCGA_Gb
Cuts at: 0 419 1776
Size: 419 1357
PstI C_TGCA'G
Cuts at: 0 213 405 668 1776
Size: 213 192 263 1108
Fragments arranged by size:
1108 263 213 192
PvuII CAG'CTG
Cuts at: 0 654 744 1776
Size: 654 90 1032
Fragments arranged by size:
1032 654 90
SacI G_AGCT'C
Cuts at: 0 481 1776
Size: 481 1295
SalI G'TCGA_C
Cuts at: 0 1183 1776
Size: 1183 593
SapI GCTCTTCn'nnn_
Cuts at: 0 1771 1776
Size: 1771 5
SfcI C'TryA_G
Cuts at: 0 209 401 664 1776
Size: 209 192 263 1112
Fragments arranged by size:
1112 263 209 192
SmlI C'TyrA_G
Cuts at: 0 419 615 757 1776
Size: 419 196 142 1019
Fragments arranged by size:
1019 419 196 142
SspI AAT'ATT
Cuts at: 0 240 1776
Size: 240 1536
StuI AGG'CCT
Cuts at: 0 1720 1776
Size: 1720 56
StyI C'CwwG_G
Cuts at: 0 500 1776
Size: 500 1276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 906 1776
Size: 906 870
TatI w'GTAC_w
Cuts at: 0 587 656 1277 1776
Size: 587 69 621 499
Fragments arranged by size:
621 587 499 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 858 1776
Size: 858 918
XhoI C'TCGA_G
Cuts at: 0 419 1776
Size: 419 1357
XmnI GAAnn'nnTTC
Cuts at: 0 532 1776
Size: 532 1244
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AvaI BanII
BbsI BglI BmgBI BmrI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoRI Hin4I HincII HindIII MmeI
MscI MslI MspA1I NdeI NspI PciI PshAI PsiI
PspXI PstI PvuII SacI SalI SapI SfcI SmlI
SspI StuI StyI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmtI BplI Bpu10I BsaAI
BsaXI BsiEI BsiWI BsmBI BspHI BssHII BssSI BstEII
BstXI BstYI Bsu36I CspCI DrdI EagI EciI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PsrI PvuI RsrII
SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI
Tth111I XbaI XmaI ZraI