(Linear) (Six-base) MAPSORT of: KIEE1520.seq  Check: 6902  from: 1  to: 354



KIEE1520.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     299     354 
   Size:        299      55
 
Acc65I G'GTAC_C

Cuts at:      0     145     354 
   Size:        145     209
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     354 
   Size:        111     243
 
AlwNI CAG_nnn'CTG

Cuts at:      0      17     354 
   Size:         17     337
 
ApoI r'AATT_y

Cuts at:      0     169     354 
   Size:        169     185
 
AvaI C'yCGr_G

Cuts at:      0      91     138     354 
   Size:         91      47     216

  Fragments arranged by size: 

                 216      91      47
 
BanI G'GyrC_C

Cuts at:      0     145     354 
   Size:        145     209
 
BanII G_rGCy'C

Cuts at:      0     266     354 
   Size:        266      88
 
BsaWI w'CCGG_w

Cuts at:      0     164     354 
   Size:        164     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     354 
   Size:        100     254
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     354 
   Size:         68     286
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     266     354 
   Size:         68     198      88

  Fragments arranged by size: 

                 198      88      68
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     299     354 
   Size:        299      55
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      17     354 
   Size:         17     337
 
BstEII G'GTnAC_C

Cuts at:      0     240     354 
   Size:        240     114
 
BstYI r'GATC_y

Cuts at:      0     219     354 
   Size:        219     135
 
BtgI C'CryG_G

Cuts at:      0     269     354 
   Size:        269      85
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     323     354 
   Size:        130     193      31

  Fragments arranged by size: 

                 193     130      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     354 
   Size:        111     243
 
FspI TGC'GCA

Cuts at:      0     295     354 
   Size:        295      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     168     200     354 
   Size:        168      32     154

  Fragments arranged by size: 

                 168     154      32
 
KpnI G_GTAC'C

Cuts at:      0     149     354 
   Size:        149     205
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     354 
   Size:        166     188
 
MspA1I CmG'CkG

Cuts at:      0      17     354 
   Size:         17     337
 
NcoI C'CATG_G

Cuts at:      0     269     354 
   Size:        269      85
 
PstI C_TGCA'G

Cuts at:      0      22     354 
   Size:         22     332
 
PvuII CAG'CTG

Cuts at:      0      17     354 
   Size:         17     337
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130     354 
   Size:        130     224
 
SfcI C'TryA_G

Cuts at:      0      18      99     354 
   Size:         18      81     255

  Fragments arranged by size: 

                 255      81      18
 
SmaI CCC'GGG

Cuts at:      0      93     140     354 
   Size:         93      47     214

  Fragments arranged by size: 

                 214      93      47
 
StyI C'CwwG_G

Cuts at:      0     214     269     354 
   Size:        214      55      85

  Fragments arranged by size: 

                 214      85      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     354 
   Size:         57     297
 
XmaI C'CCGG_G

Cuts at:      0      91     138     354 
   Size:         91      47     216

  Fragments arranged by size: 

                 216      91      47
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AlwNI     ApoI     AvaI     BanI    BanII
    BsaWI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI   BstEII    BstYI
     BtgI     EarI  Eco57MI     FspI    Hin4I     KpnI     MmeI   MspA1I
     NcoI     PstI    PvuII     SapI     SfcI     SmaI     StyI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI