(Linear) (Six-base) MAPSORT of: KIEE1518.seq  Check: 3296  from: 1  to: 456



KIEE1518.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     456 
   Size:         27     429
 
ApaI G_GGCC'C

Cuts at:      0     378     456 
   Size:        378      78
 
AseI AT'TA_AT

Cuts at:      0     208     456 
   Size:        208     248
 
BanII G_rGCy'C

Cuts at:      0     378     456 
   Size:        378      78
 
BglI GCCn_nnn'nGGC

Cuts at:      0     373     456 
   Size:        373      83
 
Bme1580I G_kGCm'C

Cuts at:      0     378     456 
   Size:        378      78
 
BsaAI yAC'GTr

Cuts at:      0      60     456 
   Size:         60     396
 
BsaWI w'CCGG_w

Cuts at:      0     386     456 
   Size:        386      70
 
BseYI C'CCAG_C

Cuts at:      0     406     456 
   Size:        406      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     456 
   Size:         31     425
 
Bsp1286I G_dGCh'C

Cuts at:      0     378     456 
   Size:        378      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      13      96     456 
   Size:         13      83     360

  Fragments arranged by size: 

                 360      83      13
 
ClaI AT'CG_AT

Cuts at:      0     204     456 
   Size:        204     252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     456 
   Size:         27     429
 
EcoO109I rG'GnC_Cy

Cuts at:      0     374     375     456 
   Size:        374       1      81

  Fragments arranged by size: 

                 374      81       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     140     172     456 
   Size:        140      32     284

  Fragments arranged by size: 

                 284     140      32
 
HindIII A'AGCT_T

Cuts at:      0     391     456 
   Size:        391      65
 
MspA1I CmG'CkG

Cuts at:      0     410     456 
   Size:        410      46
 
PmlI CAC'GTG

Cuts at:      0      60     456 
   Size:         60     396
 
PspOMI G'GGCC_C

Cuts at:      0     374     456 
   Size:        374      82
 
TatI w'GTAC_w

Cuts at:      0     189     456 
   Size:        189     267
 

 Enzymes that do cut:

     AcuI     ApaI     AseI    BanII     BglI Bme1580I    BsaAI    BsaWI
    BseYI     BsgI Bsp1286I    BtgZI     ClaI  Eco57MI EcoO109I    Hin4I
  HindIII   MspA1I     PmlI   PspOMI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI