(Linear) (Six-base) MAPSORT of: KIEE1517.seq  Check: 7410  from: 1  to: 339



KIEE1517.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     261     339 
   Size:        261      78
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      11     339 
   Size:         11     328
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     339 
   Size:         37     302
 
BseYI C'CCAG_C

Cuts at:      0     168     339 
   Size:        168     171
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     339 
   Size:        261      78
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     300     339 
   Size:        300      39
 
DraI TTT'AAA

Cuts at:      0     183     339 
   Size:        183     156
 
MfeI C'AATT_G

Cuts at:      0      83     339 
   Size:         83     256
 
SfcI C'TryA_G

Cuts at:      0      71     339 
   Size:         71     268
 
SwaI ATTT'AAAT

Cuts at:      0     183     339 
   Size:        183     156
 
TatI w'GTAC_w

Cuts at:      0     305     339 
   Size:        305      34
 

 Enzymes that do cut:

     AlfI     BbsI    BseRI    BseYI     BsgI    BtgZI     DraI     MfeI
     SfcI     SwaI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI