Restriction map for
pFN21AE1515
(Linear) (Six-base) MAPSORT of: KIEE1515.seq Check: 2473 from: 1 to: 780
KIEE1515.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AatII G_ACGT'C
Cuts at: 0 572 780
Size: 572 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 780
Size: 29 751
AhdI GACnn_n'nnGTC
Cuts at: 0 108 780
Size: 108 672
AlwNI CAG_nnn'CTG
Cuts at: 0 478 780
Size: 478 302
ApaI G_GGCC'C
Cuts at: 0 768 776 780
Size: 768 8 4
Fragments arranged by size:
768 8 4
ApoI r'AATT_y
Cuts at: 0 166 780
Size: 166 614
AvaI C'yCGr_G
Cuts at: 0 761 780
Size: 761 19
BanI G'GyrC_C
Cuts at: 0 718 780
Size: 718 62
BanII G_rGCy'C
Cuts at: 0 51 768 776 780
Size: 51 717 8 4
Fragments arranged by size:
717 51 8 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 780
Size: 17 763
BbvCI CC'TCA_GC
Cuts at: 0 558 780
Size: 558 222
BglI GCCn_nnn'nGGC
Cuts at: 0 97 704 780
Size: 97 607 76
Fragments arranged by size:
607 97 76
Bme1580I G_kGCm'C
Cuts at: 0 557 681 721 768 776 780
Size: 557 124 40 47 8 4
Fragments arranged by size:
557 124 47 40 8 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 603 633 780
Size: 603 30 147
Fragments arranged by size:
603 147 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 780
Size: 247 533
Bpu10I CC'TnA_GC
Cuts at: 0 260 558 780
Size: 260 298 222
Fragments arranged by size:
298 260 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 601 780
Size: 63 538 179
Fragments arranged by size:
538 179 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 780
Size: 205 575
BsaHI Gr'CG_yC
Cuts at: 0 569 780
Size: 569 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 780
Size: 145 30 605
Fragments arranged by size:
605 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 95 244 604 780
Size: 95 149 360 176
Fragments arranged by size:
360 176 149 95
BseYI C'CCAG_C
Cuts at: 0 85 387 693 780
Size: 85 302 306 87
Fragments arranged by size:
306 302 87 85
BsiHKAI G_wGCw'C
Cuts at: 0 443 593 674 780
Size: 443 150 81 106
Fragments arranged by size:
443 150 106 81
Bsp1286I G_dGCh'C
Cuts at: 0 51 443 557 593 674 681 721 768
Size: 51 392 114 36 81 7 40 47
Cuts at: 768 776 780
Size: 8 4
Fragments arranged by size:
392 114 81 51 47 40 36 8
7 4
BsrBI CCG'CTC
Cuts at: 0 612 780
Size: 612 168
BsrDI GCAATG_nn'
Cuts at: 0 278 468 780
Size: 278 190 312
Fragments arranged by size:
312 278 190
BsrGI T'GTAC_A
Cuts at: 0 129 780
Size: 129 651
BstEII G'GTnAC_C
Cuts at: 0 357 780
Size: 357 423
BstYI r'GATC_y
Cuts at: 0 195 780
Size: 195 585
Bsu36I CC'TnA_GG
Cuts at: 0 353 780
Size: 353 427
EaeI y'GGCC_r
Cuts at: 0 237 750 780
Size: 237 513 30
Fragments arranged by size:
513 237 30
EarI CTCTTCn'nnn_
Cuts at: 0 505 780
Size: 505 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 780
Size: 115 665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 247 780
Size: 29 218 533
Fragments arranged by size:
533 218 29
EcoO109I rG'GnC_Cy
Cuts at: 0 772 773 780
Size: 772 1 7
Fragments arranged by size:
772 7 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 94 126 780
Size: 23 32 39 32 654
Fragments arranged by size:
654 39 32 32 23
HincII GTy'rAC
Cuts at: 0 113 780
Size: 113 667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 780
Size: 347 433
MscI TGG'CCA
Cuts at: 0 752 780
Size: 752 28
MslI CAynn'nnrTG
Cuts at: 0 456 712 780
Size: 456 256 68
Fragments arranged by size:
456 256 68
PasI CC'CwG_GG
Cuts at: 0 681 768 780
Size: 681 87 12
Fragments arranged by size:
681 87 12
PspOMI G'GGCC_C
Cuts at: 0 764 772 780
Size: 764 8 8
Fragments arranged by size:
764 8 8
PstI C_TGCA'G
Cuts at: 0 583 780
Size: 583 197
SfcI C'TryA_G
Cuts at: 0 579 780
Size: 579 201
SmaI CCC'GGG
Cuts at: 0 763 780
Size: 763 17
SmlI C'TyrA_G
Cuts at: 0 42 616 780
Size: 42 574 164
Fragments arranged by size:
574 164 42
StyI C'CwwG_G
Cuts at: 0 56 371 780
Size: 56 315 409
Fragments arranged by size:
409 315 56
TatI w'GTAC_w
Cuts at: 0 129 780
Size: 129 651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 780
Size: 378 402
XmaI C'CCGG_G
Cuts at: 0 761 780
Size: 761 19
ZraI GAC'GTC
Cuts at: 0 570 780
Size: 570 210
Enzymes that do cut:
AatII AcuI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BsrBI BsrDI BsrGI BstEII BstYI Bsu36I EaeI EarI
EciI Eco57MI EcoO109I Hin4I HincII MmeI MscI MslI
PasI PspOMI PstI SfcI SmaI SmlI StyI TatI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI