(Linear) (Six-base) MAPSORT of: KIEE1513.seq  Check: 3703  from: 1  to: 543



KIEE1513.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     543 
   Size:        352     191
 
AhdI GACnn_n'nnGTC

Cuts at:      0      63     543 
   Size:         63     480
 
AlwNI CAG_nnn'CTG

Cuts at:      0      23     543 
   Size:         23     520
 
BamHI G'GATC_C

Cuts at:      0     243     543 
   Size:        243     300
 
BbvCI CC'TCA_GC

Cuts at:      0     158     543 
   Size:        158     385
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     370     543 
   Size:        370     173
 
BfrBI ATG'CAT

Cuts at:      0      77     543 
   Size:         77     466
 
BglI GCCn_nnn'nGGC

Cuts at:      0     371     543 
   Size:        371     172
 
BlpI GC'TnA_GC

Cuts at:      0      93     543 
   Size:         93     450
 
Bme1580I G_kGCm'C

Cuts at:      0      33     543 
   Size:         33     510
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     543 
   Size:        215     328
 
Bpu10I CC'TnA_GC

Cuts at:      0     158     543 
   Size:        158     385
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     543 
   Size:        466      77
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     205     543 
   Size:        205     338
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     543 
   Size:         33     510
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      23     543 
   Size:         23     520
 
BstEII G'GTnAC_C

Cuts at:      0     513     543 
   Size:        513      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      45      70     525     543 
   Size:         45      25     455      18

  Fragments arranged by size: 

                 455      45      25      18
 
BstYI r'GATC_y

Cuts at:      0     243     543 
   Size:        243     300
 
DraI TTT'AAA

Cuts at:      0     221     543 
   Size:        221     322
 
EaeI y'GGCC_r

Cuts at:      0      35     543 
   Size:         35     508
 
EarI CTCTTCn'nnn_

Cuts at:      0     146     543 
   Size:        146     397
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     352     543 
   Size:        352     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     352     543 
   Size:        215     137     191

  Fragments arranged by size: 

                 215     191     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     323     543 
   Size:        323     220
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     543 
   Size:         49      32     462

  Fragments arranged by size: 

                 462      49      32
 
HindIII A'AGCT_T

Cuts at:      0     536     543 
   Size:        536       7
 
MfeI C'AATT_G

Cuts at:      0      50     543 
   Size:         50     493
 
MscI TGG'CCA

Cuts at:      0      37     543 
   Size:         37     506
 
MspA1I CmG'CkG

Cuts at:      0     228     543 
   Size:        228     315
 
NsiI A_TGCA'T

Cuts at:      0      79     543 
   Size:         79     464
 
PpuMI rG'GwC_Cy

Cuts at:      0     323     543 
   Size:        323     220
 
PvuII CAG'CTG

Cuts at:      0     228     543 
   Size:        228     315
 
SmlI C'TyrA_G

Cuts at:      0     445     543 
   Size:        445      98
 
TatI w'GTAC_w

Cuts at:      0     130     543 
   Size:        130     413
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     243     275     543 
   Size:        243      32     268

  Fragments arranged by size: 

                 268     243      32
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI    BamHI    BbvCI    BciVI    BfrBI     BglI
     BlpI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI Bsp1286I   BstAPI
   BstEII    BstXI    BstYI     DraI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    Hin4I  HindIII     MfeI     MscI   MspA1I     NsiI    PpuMI
    PvuII     SmlI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI     BcgI     BclI    BglII    BmgBI     BmrI     BmtI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI