Restriction map for
pFN21AE1510
(Linear) (Six-base) MAPSORT of: KIEE1510.seq Check: 320 from: 1 to: 2145
KIEE1510.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1524 2145
Size: 1524 621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 857 959 1847 1943 2032 2145
Size: 583 274 102 888 96 89 113
Fragments arranged by size:
888 583 274 113 102 96 89
AfeI AGC'GCT
Cuts at: 0 105 2145
Size: 105 2040
AflII C'TTAA_G
Cuts at: 0 1731 2145
Size: 1731 414
AflIII A'CryG_T
Cuts at: 0 420 1313 2145
Size: 420 893 832
Fragments arranged by size:
893 832 420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 2145
Size: 295 1850
AlwNI CAG_nnn'CTG
Cuts at: 0 232 2145
Size: 232 1913
ApoI r'AATT_y
Cuts at: 0 518 875 943 980 1109 1252 1596 1605
Size: 518 357 68 37 129 143 344 9
Cuts at: 1605 1770 2145
Size: 165 375
Fragments arranged by size:
518 375 357 344 165 143 129 68
37 9
AvaI C'yCGr_G
Cuts at: 0 32 2145
Size: 32 2113
BamHI G'GATC_C
Cuts at: 0 1223 1736 2145
Size: 1223 513 409
Fragments arranged by size:
1223 513 409
BanI G'GyrC_C
Cuts at: 0 87 511 1512 2145
Size: 87 424 1001 633
Fragments arranged by size:
1001 633 424 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 937 1835 2145
Size: 937 898 310
Fragments arranged by size:
937 898 310
Bme1580I G_kGCm'C
Cuts at: 0 92 98 2145
Size: 92 6 2047
Fragments arranged by size:
2047 92 6
BmtI G_CTAG'C
Cuts at: 0 1676 2145
Size: 1676 469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 100 377 1059 2145
Size: 84 16 277 682 1086
Fragments arranged by size:
1086 682 277 84 16
Bpu10I CC'TnA_GC
Cuts at: 0 463 2145
Size: 463 1682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2053 2145
Size: 2053 92
BsaAI yAC'GTr
Cuts at: 0 1316 2145
Size: 1316 829
BsaBI GATnn'nnATC
Cuts at: 0 902 1049 2145
Size: 902 147 1096
Fragments arranged by size:
1096 902 147
BsaWI w'CCGG_w
Cuts at: 0 1544 2145
Size: 1544 601
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1696 1699 1765 1774 2145
Size: 1696 3 66 9 371
Fragments arranged by size:
1696 371 66 9 3
BseYI C'CCAG_C
Cuts at: 0 467 1157 2126 2145
Size: 467 690 969 19
Fragments arranged by size:
969 690 467 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 169 301 1426 2145
Size: 130 39 132 1125 719
Fragments arranged by size:
1125 719 132 130 39
BsmI GAATG_Cn'
Cuts at: 0 587 847 2145
Size: 587 260 1298
Fragments arranged by size:
1298 587 260
Bsp1286I G_dGCh'C
Cuts at: 0 92 98 2145
Size: 92 6 2047
Fragments arranged by size:
2047 92 6
BspEI T'CCGG_A
Cuts at: 0 1544 2145
Size: 1544 601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 1524 2145
Size: 48 1476 621
Fragments arranged by size:
1476 621 48
BsrBI CCG'CTC
Cuts at: 0 1470 2145
Size: 1470 675
BsrDI GCAATG_nn'
Cuts at: 0 830 932 2145
Size: 830 102 1213
Fragments arranged by size:
1213 830 102
BssSI C'ACGA_G
Cuts at: 0 540 1337 2145
Size: 540 797 808
Fragments arranged by size:
808 797 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1575 2145
Size: 1575 570
BstYI r'GATC_y
Cuts at: 0 1223 1409 1736 2145
Size: 1223 186 327 409
Fragments arranged by size:
1223 409 327 186
BtgI C'CryG_G
Cuts at: 0 858 2145
Size: 858 1287
DrdI GACnn_nn'nnGTC
Cuts at: 0 121 2145
Size: 121 2024
EaeI y'GGCC_r
Cuts at: 0 152 2145
Size: 152 1993
EarI CTCTTCn'nnn_
Cuts at: 0 17 1880 2046 2145
Size: 17 1863 166 99
Fragments arranged by size:
1863 166 99 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 100 377 583 857 959 1059 1847
Size: 84 16 277 206 274 102 100 788
Cuts at: 1847 1943 2032 2145
Size: 96 89 113
Fragments arranged by size:
788 277 274 206 113 102 100 96
89 84 16
EcoRI G'AATT_C
Cuts at: 0 1252 1605 1770 2145
Size: 1252 353 165 375
Fragments arranged by size:
1252 375 353 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 478 2145
Size: 478 1667
HaeII r_GCGC'y
Cuts at: 0 107 2145
Size: 107 2038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1225 1257 1292 1324 2145
Size: 1225 32 35 32 821
Fragments arranged by size:
1225 821 35 32 32
HincII GTy'rAC
Cuts at: 0 1233 2145
Size: 1233 912
MfeI C'AATT_G
Cuts at: 0 2069 2145
Size: 2069 76
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 2145
Size: 1358 787
MslI CAynn'nnrTG
Cuts at: 0 784 2145
Size: 784 1361
MspA1I CmG'CkG
Cuts at: 0 229 937 2145
Size: 229 708 1208
Fragments arranged by size:
1208 708 229
NdeI CA'TA_TG
Cuts at: 0 1245 2145
Size: 1245 900
NheI G'CTAG_C
Cuts at: 0 1672 2145
Size: 1672 473
NspI r_CATG'y
Cuts at: 0 424 2145
Size: 424 1721
PciI A'CATG_T
Cuts at: 0 420 2145
Size: 420 1725
PflMI CCAn_nnn'nTGG
Cuts at: 0 2141 2145
Size: 2141 4
PfoI T'CCnGG_A
Cuts at: 0 1893 2145
Size: 1893 252
PmlI CAC'GTG
Cuts at: 0 1316 2145
Size: 1316 829
PsiI TTA'TAA
Cuts at: 0 661 2145
Size: 661 1484
PstI C_TGCA'G
Cuts at: 0 59 2145
Size: 59 2086
PvuII CAG'CTG
Cuts at: 0 229 937 2145
Size: 229 708 1208
Fragments arranged by size:
1208 708 229
SexAI A'CCwGG_T
Cuts at: 0 137 572 2145
Size: 137 435 1573
Fragments arranged by size:
1573 435 137
SfcI C'TryA_G
Cuts at: 0 55 949 2145
Size: 55 894 1196
Fragments arranged by size:
1196 894 55
SmaI CCC'GGG
Cuts at: 0 34 2145
Size: 34 2111
SmlI C'TyrA_G
Cuts at: 0 1731 2032 2145
Size: 1731 301 113
Fragments arranged by size:
1731 301 113
SrfI GCCC'GGGC
Cuts at: 0 34 2145
Size: 34 2111
StyI C'CwwG_G
Cuts at: 0 507 1389 1422 1776 2145
Size: 507 882 33 354 369
Fragments arranged by size:
882 507 369 354 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 1373 1921 2115 2145
Size: 256 1117 548 194 30
Fragments arranged by size:
1117 548 256 194 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 441 473 1388 1420 2145
Size: 441 32 915 32 725
Fragments arranged by size:
915 725 441 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 580 1243 1763 2145
Size: 580 663 520 382
Fragments arranged by size:
663 580 520 382
XmaI C'CCGG_G
Cuts at: 0 32 2145
Size: 32 2113
XmnI GAAnn'nnTTC
Cuts at: 0 944 1889 2145
Size: 944 945 256
Fragments arranged by size:
945 944 256
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AlfI AlwNI ApoI
AvaI BamHI BanI BbsI Bme1580I BmtI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstXI BstYI
BtgI DrdI EaeI EarI Eco57MI EcoRI FalI HaeII
Hin4I HincII MfeI MmeI MslI MspA1I NdeI NheI
NspI PciI PflMI PfoI PmlI PsiI PstI PvuII
SexAI SfcI SmaI SmlI SrfI StyI TaqII TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BplI BsaI BsaHI BsaXI BsiEI
BsiHKAI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI
PacI PasI PmeI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TatI Tth111I XbaI XhoI
ZraI