Restriction map for
pFN21AE1509
(Linear) (Six-base) MAPSORT of: KIEE1509.seq Check: 6457 from: 1 to: 1098
KIEE1509.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AflIII A'CryG_T
Cuts at: 0 633 842 1098
Size: 633 209 256
Fragments arranged by size:
633 256 209
AleI CACnn'nnGTG
Cuts at: 0 103 562 1098
Size: 103 459 536
Fragments arranged by size:
536 459 103
ApaI G_GGCC'C
Cuts at: 0 386 687 1098
Size: 386 301 411
Fragments arranged by size:
411 386 301
AvaI C'yCGr_G
Cuts at: 0 39 1098
Size: 39 1059
AvrII C'CTAG_G
Cuts at: 0 969 1098
Size: 969 129
BanI G'GyrC_C
Cuts at: 0 12 216 392 816 1098
Size: 12 204 176 424 282
Fragments arranged by size:
424 282 204 176 12
BanII G_rGCy'C
Cuts at: 0 48 131 386 687 878 1012 1069 1098
Size: 48 83 255 301 191 134 57 29
Fragments arranged by size:
301 255 191 134 83 57 48 29
BbeI G_GCGC'C
Cuts at: 0 16 220 396 1098
Size: 16 204 176 702
Fragments arranged by size:
702 204 176 16
BglI GCCn_nnn'nGGC
Cuts at: 0 390 526 1098
Size: 390 136 572
Fragments arranged by size:
572 390 136
Bme1580I G_kGCm'C
Cuts at: 0 386 559 687 1062 1098
Size: 386 173 128 375 36
Fragments arranged by size:
386 375 173 128 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1052 1098
Size: 1052 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 571 607 845 1098
Size: 340 231 36 238 253
Fragments arranged by size:
340 253 238 231 36
Bpu10I CC'TnA_GC
Cuts at: 0 1011 1098
Size: 1011 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 350 760 1098
Size: 55 295 410 338
Fragments arranged by size:
410 338 295 55
BsaAI yAC'GTr
Cuts at: 0 153 560 636 845 1098
Size: 153 407 76 209 253
Fragments arranged by size:
407 253 209 153 76
BsaHI Gr'CG_yC
Cuts at: 0 13 188 217 393 1098
Size: 13 175 29 176 705
Fragments arranged by size:
705 176 175 29 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 544 574 965 995 1098
Size: 448 30 66 30 391 30 103
Fragments arranged by size:
448 391 103 66 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 1098
Size: 388 710
BseYI C'CCAG_C
Cuts at: 0 500 680 905 1098
Size: 500 180 225 193
Fragments arranged by size:
500 225 193 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1098
Size: 874 224
BsiEI CG_ry'CG
Cuts at: 0 120 1098
Size: 120 978
BsiHKAI G_wGCw'C
Cuts at: 0 1012 1098
Size: 1012 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 595 657 1098
Size: 595 62 441
Fragments arranged by size:
595 441 62
Bsp1286I G_dGCh'C
Cuts at: 0 48 131 386 559 687 878 1012 1062
Size: 48 83 255 173 128 191 134 50
Cuts at: 1062 1069 1098
Size: 7 29
Fragments arranged by size:
255 191 173 134 128 83 50 48
29 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 467 1098
Size: 320 147 631
Fragments arranged by size:
631 320 147
BssHII G'CGCG_C
Cuts at: 0 612 1098
Size: 612 486
BssSI C'ACGA_G
Cuts at: 0 859 1098
Size: 859 239
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 1098
Size: 171 927
Bsu36I CC'TnA_GG
Cuts at: 0 673 1098
Size: 673 425
BtgI C'CryG_G
Cuts at: 0 120 211 220 402 476 1098
Size: 120 91 9 182 74 622
Fragments arranged by size:
622 182 120 91 74 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 426 1068 1098
Size: 123 303 642 30
Fragments arranged by size:
642 303 123 30
BtsI GCAGTG_nn'
Cuts at: 0 149 1098
Size: 149 949
EaeI y'GGCC_r
Cuts at: 0 117 1098
Size: 117 981
EagI C'GGCC_G
Cuts at: 0 117 1098
Size: 117 981
EarI CTCTTCn'nnn_
Cuts at: 0 824 1000 1098
Size: 824 176 98
Fragments arranged by size:
824 176 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 408 544 1098
Size: 408 136 554
Fragments arranged by size:
554 408 136
EcoICRI GAG'CTC
Cuts at: 0 1010 1098
Size: 1010 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 571 607 845 1098
Size: 340 231 36 238 253
Fragments arranged by size:
340 253 238 231 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 1098
Size: 451 647
EcoO109I rG'GnC_Cy
Cuts at: 0 382 456 684 1098
Size: 382 74 228 414
Fragments arranged by size:
414 382 228 74
HaeII r_GCGC'y
Cuts at: 0 16 220 279 396 1098
Size: 16 204 59 117 702
Fragments arranged by size:
702 204 117 59 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 448 480 630 662 1098
Size: 328 32 88 32 150 32 436
Fragments arranged by size:
436 328 150 88 32 32 32
KasI G'GCGC_C
Cuts at: 0 12 216 392 1098
Size: 12 204 176 706
Fragments arranged by size:
706 204 176 12
MslI CAynn'nnrTG
Cuts at: 0 103 142 562 1098
Size: 103 39 420 536
Fragments arranged by size:
536 420 103 39
MspA1I CmG'CkG
Cuts at: 0 122 213 404 478 726 808 1098
Size: 122 91 191 74 248 82 290
Fragments arranged by size:
290 248 191 122 91 82 74
NarI GG'CG_CC
Cuts at: 0 13 217 393 1098
Size: 13 204 176 705
Fragments arranged by size:
705 204 176 13
PasI CC'CwG_GG
Cuts at: 0 687 1062 1098
Size: 687 375 36
Fragments arranged by size:
687 375 36
PfoI T'CCnGG_A
Cuts at: 0 466 1098
Size: 466 632
PmlI CAC'GTG
Cuts at: 0 560 636 845 1098
Size: 560 76 209 253
Fragments arranged by size:
560 253 209 76
PpuMI rG'GwC_Cy
Cuts at: 0 456 1098
Size: 456 642
PspOMI G'GGCC_C
Cuts at: 0 382 683 1098
Size: 382 301 415
Fragments arranged by size:
415 382 301
PstI C_TGCA'G
Cuts at: 0 611 1098
Size: 611 487
PvuII CAG'CTG
Cuts at: 0 808 1098
Size: 808 290
SacI G_AGCT'C
Cuts at: 0 1012 1098
Size: 1012 86
SacII CC_GC'GG
Cuts at: 0 123 214 405 479 1098
Size: 123 91 191 74 619
Fragments arranged by size:
619 191 123 91 74
SapI GCTCTTCn'nnn_
Cuts at: 0 824 1000 1098
Size: 824 176 98
Fragments arranged by size:
824 176 98
SfcI C'TryA_G
Cuts at: 0 607 1098
Size: 607 491
SfoI GGC'GCC
Cuts at: 0 14 218 394 1098
Size: 14 204 176 704
Fragments arranged by size:
704 204 176 14
StyI C'CwwG_G
Cuts at: 0 969 1098
Size: 969 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 213 308 514 638 1098
Size: 73 140 95 206 124 460
Fragments arranged by size:
460 206 140 124 95 73
TatI w'GTAC_w
Cuts at: 0 269 1098
Size: 269 829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 965 997 1098
Size: 544 32 389 32 101
Fragments arranged by size:
544 389 101 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 921 1098
Size: 364 557 177
Fragments arranged by size:
557 364 177
Enzymes that do cut:
AflIII AleI ApaI AvaI AvrII BanI BanII BbeI
BglI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BssHII BssSI BstAPI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MslI MspA1I NarI PasI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI
BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BstBI BstEII BstXI BstYI BstZ17I ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI