(Linear) (Six-base) MAPSORT of: KIEE1508.seq  Check: 6954  from: 1  to: 426



KIEE1508.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AvaI C'yCGr_G

Cuts at:      0     150     426 
   Size:        150     276
 
BanI G'GyrC_C

Cuts at:      0      76     235     426 
   Size:         76     159     191

  Fragments arranged by size: 

                 191     159      76
 
BbeI G_GCGC'C

Cuts at:      0     239     426 
   Size:        239     187
 
BbvCI CC'TCA_GC

Cuts at:      0     103     426 
   Size:        103     323
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     408     426 
   Size:        408      18
 
BglII A'GATC_T

Cuts at:      0      50     426 
   Size:         50     376
 
BlpI GC'TnA_GC

Cuts at:      0     250     426 
   Size:        250     176
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     426 
   Size:        109     317
 
Bpu10I CC'TnA_GC

Cuts at:      0     103     426 
   Size:        103     323
 
BsaHI Gr'CG_yC

Cuts at:      0     236     426 
   Size:        236     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     244     426 
   Size:        130     114     182

  Fragments arranged by size: 

                 182     130     114
 
BsiEI CG_ry'CG

Cuts at:      0     359     395     426 
   Size:        359      36      31

  Fragments arranged by size: 

                 359      36      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     152     426 
   Size:        152     274
 
BssHII G'CGCG_C

Cuts at:      0     168     170     334     426 
   Size:        168       2     164      92

  Fragments arranged by size: 

                 168     164      92       2
 
BstEII G'GTnAC_C

Cuts at:      0     321     426 
   Size:        321     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     189     426 
   Size:        189     237
 
BstYI r'GATC_y

Cuts at:      0      50     426 
   Size:         50     376
 
BtgI C'CryG_G

Cuts at:      0     197     426 
   Size:        197     229
 
BtsI GCAGTG_nn'

Cuts at:      0     309     426 
   Size:        309     117
 
EaeI y'GGCC_r

Cuts at:      0     191     329     356     426 
   Size:        191     138      27      70

  Fragments arranged by size: 

                 191     138      70      27
 
EagI C'GGCC_G

Cuts at:      0     356     426 
   Size:        356      70
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     208     426 
   Size:        103     105     218

  Fragments arranged by size: 

                 218     105     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     426 
   Size:        109     317
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     144     426 
   Size:        144     282
 
HaeII r_GCGC'y

Cuts at:      0     239     426 
   Size:        239     187
 
HincII GTy'rAC

Cuts at:      0     291     426 
   Size:        291     135
 
KasI G'GCGC_C

Cuts at:      0     235     426 
   Size:        235     191
 
MslI CAynn'nnrTG

Cuts at:      0      31     426 
   Size:         31     395
 
NarI GG'CG_CC

Cuts at:      0     236     426 
   Size:        236     190
 
SapI GCTCTTCn'nnn_

Cuts at:      0     103     208     426 
   Size:        103     105     218

  Fragments arranged by size: 

                 218     105     103
 
SfoI GGC'GCC

Cuts at:      0     237     426 
   Size:        237     189
 
SmaI CCC'GGG

Cuts at:      0     152     426 
   Size:        152     274
 
StyI C'CwwG_G

Cuts at:      0     182     426 
   Size:        182     244
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     381     426 
   Size:        381      45
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     139     171     426 
   Size:        139      32     255

  Fragments arranged by size: 

                 255     139      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     286     426 
   Size:        286     140
 
XmaI C'CCGG_G

Cuts at:      0     150     426 
   Size:        150     276
 

 Enzymes that do cut:

     AvaI     BanI     BbeI    BbvCI    BciVI    BglII     BlpI     BpmI
   Bpu10I    BsaHI     BsgI    BsiEI    BspMI   BssHII   BstEII    BstXI
    BstYI     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI     FalI
    HaeII   HincII     KasI     MslI     NarI     SapI     SfoI     SmaI
     StyI    TaqII     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbsI     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XcmI     XhoI     XmnI     ZraI