Restriction map for
pFN21AE1504
(Linear) (Six-base) MAPSORT of: KIEE1504.seq Check: 6588 from: 1 to: 3507
KIEE1504.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 234 1914 3507
Size: 234 1680 1593
Fragments arranged by size:
1680 1593 234
AatII G_ACGT'C
Cuts at: 0 2651 3101 3507
Size: 2651 450 406
Fragments arranged by size:
2651 450 406
AccI GT'mk_AC
Cuts at: 0 292 1400 1658 2771 3507
Size: 292 1108 258 1113 736
Fragments arranged by size:
1113 1108 736 292 258
AclI AA'CG_TT
Cuts at: 0 1980 3507
Size: 1980 1527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1050 2143 2220 2337 3093 3160 3507
Size: 1006 44 1093 77 117 756 67 347
Fragments arranged by size:
1093 1006 756 347 117 77 67 44
AfeI AGC'GCT
Cuts at: 0 102 1071 3507
Size: 102 969 2436
Fragments arranged by size:
2436 969 102
AflIII A'CryG_T
Cuts at: 0 266 356 1175 2539 3507
Size: 266 90 819 1364 968
Fragments arranged by size:
1364 968 819 266 90
AleI CACnn'nnGTG
Cuts at: 0 1312 2212 3229 3507
Size: 1312 900 1017 278
Fragments arranged by size:
1312 1017 900 278
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 3507
Size: 442 32 3033
Fragments arranged by size:
3033 442 32
AlwNI CAG_nnn'CTG
Cuts at: 0 315 1257 1761 1944 2827 3022 3507
Size: 315 942 504 183 883 195 485
Fragments arranged by size:
942 883 504 485 315 195 183
ApaI G_GGCC'C
Cuts at: 0 391 738 1272 1440 1629 1818 3507
Size: 391 347 534 168 189 189 1689
Fragments arranged by size:
1689 534 391 347 189 189 168
ApaLI G'TGCA_C
Cuts at: 0 334 706 3507
Size: 334 372 2801
Fragments arranged by size:
2801 372 334
ApoI r'AATT_y
Cuts at: 0 586 613 755 885 3507
Size: 586 27 142 130 2622
Fragments arranged by size:
2622 586 142 130 27
AvaI C'yCGr_G
Cuts at: 0 1460 1817 2116 2157 3507
Size: 1460 357 299 41 1350
Fragments arranged by size:
1460 1350 357 299 41
BanI G'GyrC_C
Cuts at: 0 220 246 1132 2569 2617 2916 3507
Size: 220 26 886 1437 48 299 591
Fragments arranged by size:
1437 886 591 299 220 48 26
BanII G_rGCy'C
Cuts at: 0 159 233 391 738 1162 1272 1440 1629
Size: 159 74 158 347 424 110 168 189
Cuts at: 1629 1723 1818 2753 3273 3507
Size: 94 95 935 520 234
Fragments arranged by size:
935 520 424 347 234 189 168 159
158 110 95 94 74
BbeI G_GCGC'C
Cuts at: 0 2621 3507
Size: 2621 886
BbsI GAAGACnn'nnnn_
Cuts at: 0 2441 3507
Size: 2441 1066
BbvCI CC'TCA_GC
Cuts at: 0 637 3151 3507
Size: 637 2514 356
Fragments arranged by size:
2514 637 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3267 3301 3507
Size: 3267 34 206
Fragments arranged by size:
3267 206 34
BglII A'GATC_T
Cuts at: 0 995 3507
Size: 995 2512
Bme1580I G_kGCm'C
Cuts at: 0 251 338 391 436 710 738 1272 1440
Size: 251 87 53 45 274 28 534 168
Cuts at: 1440 1629 1818 2572 3507
Size: 189 189 754 935
Fragments arranged by size:
935 754 534 274 251 189 189 168
87 53 45 28
BmgBI CAC'GTC
Cuts at: 0 1614 3507
Size: 1614 1893
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 2548 3507
Size: 552 1996 959
Fragments arranged by size:
1996 959 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1719 2416 2595 2767 3310 3507
Size: 1148 571 697 179 172 543 197
Fragments arranged by size:
1148 697 571 543 197 179 172
Bpu10I CC'TnA_GC
Cuts at: 0 39 474 637 1837 3151 3507
Size: 39 435 163 1200 1314 356
Fragments arranged by size:
1314 1200 435 356 163 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 540 1012 1935 2666 2967 3507
Size: 540 472 923 731 301 540
Fragments arranged by size:
923 731 540 540 472 301
BsaAI yAC'GTr
Cuts at: 0 2493 3507
Size: 2493 1014
BsaHI Gr'CG_yC
Cuts at: 0 289 2618 2648 3098 3507
Size: 289 2329 30 450 409
Fragments arranged by size:
2329 450 409 289 30
BsaWI w'CCGG_w
Cuts at: 0 367 2090 3507
Size: 367 1723 1417
Fragments arranged by size:
1723 1417 367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 104 134 2929 2959 3088 3118 3507
Size: 104 30 2795 30 129 30 389
Fragments arranged by size:
2795 389 129 104 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 88 477 1722 2845 2985 3136 3439
Size: 13 75 389 1245 1123 140 151 303
Cuts at: 3439 3507
Size: 68
Fragments arranged by size:
1245 1123 389 303 151 140 75 68
13
BseYI C'CCAG_C
Cuts at: 0 103 308 395 550 1581 1684 1724 3015
Size: 103 205 87 155 1031 103 40 1291
Cuts at: 3015 3161 3239 3469 3507
Size: 146 78 230 38
Fragments arranged by size:
1291 1031 230 205 155 146 103 103
87 78 40 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 2515 3507
Size: 1270 1245 992
Fragments arranged by size:
1270 1245 992
BsiEI CG_ry'CG
Cuts at: 0 3197 3507
Size: 3197 310
BsiHKAI G_wGCw'C
Cuts at: 0 338 710 1832 1856 2581 2753 2995 3273
Size: 338 372 1122 24 725 172 242 278
Cuts at: 3273 3507
Size: 234
Fragments arranged by size:
1122 725 372 338 278 242 234 172
24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2655 3507
Size: 2655 852
Bsp1286I G_dGCh'C
Cuts at: 0 159 233 251 338 391 436 710 738
Size: 159 74 18 87 53 45 274 28
Cuts at: 738 1162 1272 1440 1629 1723 1818 1832 1856
Size: 424 110 168 189 94 95 14 24
Cuts at: 1856 2572 2581 2753 2995 3273 3507
Size: 716 9 172 242 278 234
Fragments arranged by size:
716 424 278 274 242 234 189 172
168 159 110 95 94 87 74 53
45 28 24 18 14 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 234 1893 1914 2000 2156 2252 2900 3507
Size: 234 1659 21 86 156 96 648 607
Fragments arranged by size:
1659 648 607 234 156 96 86 21
BsrBI CCG'CTC
Cuts at: 0 1766 3507
Size: 1766 1741
BsrDI GCAATG_nn'
Cuts at: 0 908 1330 3068 3507
Size: 908 422 1738 439
Fragments arranged by size:
1738 908 439 422
BsrFI r'CCGG_y
Cuts at: 0 2675 3507
Size: 2675 832
BsrGI T'GTAC_A
Cuts at: 0 3380 3507
Size: 3380 127
BssSI C'ACGA_G
Cuts at: 0 340 3507
Size: 340 3167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2585 3507
Size: 2585 922
BstBI TT'CG_AA
Cuts at: 0 1040 3507
Size: 1040 2467
BstXI CCAn_nnnn'nTGG
Cuts at: 0 190 1230 3507
Size: 190 1040 2277
Fragments arranged by size:
2277 1040 190
BstYI r'GATC_y
Cuts at: 0 995 1892 3507
Size: 995 897 1615
Fragments arranged by size:
1615 995 897
Bsu36I CC'TnA_GG
Cuts at: 0 3494 3507
Size: 3494 13
BtgI C'CryG_G
Cuts at: 0 821 1391 2610 3507
Size: 821 570 1219 897
Fragments arranged by size:
1219 897 821 570
BtsI GCAGTG_nn'
Cuts at: 0 2709 3507
Size: 2709 798
ClaI AT'CG_AT
Cuts at: 0 467 3507
Size: 467 3040
DraI TTT'AAA
Cuts at: 0 942 3507
Size: 942 2565
DraIII CAC_nnn'GTG
Cuts at: 0 2134 3507
Size: 2134 1373
DrdI GACnn_nn'nnGTC
Cuts at: 0 121 2896 3507
Size: 121 2775 611
Fragments arranged by size:
2775 611 121
EaeI y'GGCC_r
Cuts at: 0 683 2049 2258 2849 3507
Size: 683 1366 209 591 658
Fragments arranged by size:
1366 683 658 591 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3157 3507
Size: 3157 350
EcoICRI GAG'CTC
Cuts at: 0 2751 3271 3507
Size: 2751 520 236
Fragments arranged by size:
2751 520 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1050 1148 1719 2143 2220 2337 2416
Size: 1006 44 98 571 424 77 117 79
Cuts at: 2416 2595 2767 3093 3160 3310 3507
Size: 179 172 326 67 150 197
Fragments arranged by size:
1006 571 424 326 197 179 172 150
117 98 79 77 67 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1491 1783 3507
Size: 1491 292 1724
Fragments arranged by size:
1724 1491 292
EcoO109I rG'GnC_Cy
Cuts at: 0 388 734 1268 1269 1376 1436 1437 1625
Size: 388 346 534 1 107 60 1 188
Cuts at: 1625 1626 1681 1814 2889 3507
Size: 1 55 133 1075 618
Fragments arranged by size:
1075 618 534 388 346 188 133 107
60 55 1 1 1
EcoRI G'AATT_C
Cuts at: 0 613 3507
Size: 613 2894
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2754 3507
Size: 2754 753
HaeII r_GCGC'y
Cuts at: 0 104 1073 2552 2621 3507
Size: 104 969 1479 69 886
Fragments arranged by size:
1479 969 886 104 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1673 1705 1755 1787 1838 1870
Size: 898 32 743 32 50 32 51 32
Cuts at: 1870 2096 2128 2852 2884 3088 3120 3507
Size: 226 32 724 32 204 32 387
Fragments arranged by size:
898 743 724 387 226 204 51 50
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 2617 3507
Size: 2617 890
MfeI C'AATT_G
Cuts at: 0 854 3507
Size: 854 2653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 605 843 1979 3507
Size: 132 473 238 1136 1528
Fragments arranged by size:
1528 1136 473 238 132
MscI TGG'CCA
Cuts at: 0 2851 3507
Size: 2851 656
MslI CAynn'nnrTG
Cuts at: 0 508 820 1174 1312 2212 3229 3507
Size: 508 312 354 138 900 1017 278
Fragments arranged by size:
1017 900 508 354 312 278 138
MspA1I CmG'CkG
Cuts at: 0 175 312 663 1710 2520 2585 2745 3092
Size: 175 137 351 1047 810 65 160 347
Cuts at: 3092 3373 3507
Size: 281 134
Fragments arranged by size:
1047 810 351 347 281 175 160 137
134 65
NarI GG'CG_CC
Cuts at: 0 2618 3507
Size: 2618 889
NcoI C'CATG_G
Cuts at: 0 821 3507
Size: 821 2686
NdeI CA'TA_TG
Cuts at: 0 729 3507
Size: 729 2778
NspI r_CATG'y
Cuts at: 0 270 360 1179 1875 2543 3507
Size: 270 90 819 696 668 964
Fragments arranged by size:
964 819 696 668 270 90
PasI CC'CwG_GG
Cuts at: 0 273 1493 1550 3507
Size: 273 1220 57 1957
Fragments arranged by size:
1957 1220 273 57
PciI A'CATG_T
Cuts at: 0 266 356 1175 2539 3507
Size: 266 90 819 1364 968
Fragments arranged by size:
1364 968 819 266 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 140 315 1325 1499 3507
Size: 140 175 1010 174 2008
Fragments arranged by size:
2008 1010 175 174 140
PfoI T'CCnGG_A
Cuts at: 0 2075 3116 3246 3507
Size: 2075 1041 130 261
Fragments arranged by size:
2075 1041 261 130
PpuMI rG'GwC_Cy
Cuts at: 0 1681 2889 3507
Size: 1681 1208 618
Fragments arranged by size:
1681 1208 618
PsiI TTA'TAA
Cuts at: 0 806 3507
Size: 806 2701
PspOMI G'GGCC_C
Cuts at: 0 387 734 1268 1436 1625 1814 3507
Size: 387 347 534 168 189 189 1693
Fragments arranged by size:
1693 534 387 347 189 189 168
PstI C_TGCA'G
Cuts at: 0 245 1745 2590 3507
Size: 245 1500 845 917
Fragments arranged by size:
1500 917 845 245
PvuII CAG'CTG
Cuts at: 0 175 312 663 2520 2585 2745 3507
Size: 175 137 351 1857 65 160 762
Fragments arranged by size:
1857 762 351 175 160 137 65
SacI G_AGCT'C
Cuts at: 0 2753 3273 3507
Size: 2753 520 234
Fragments arranged by size:
2753 520 234
SanDI GG'GwC_CC
Cuts at: 0 1681 3507
Size: 1681 1826
SbfI CC_TGCA'GG
Cuts at: 0 245 3507
Size: 245 3262
SexAI A'CCwGG_T
Cuts at: 0 1120 1421 2864 3507
Size: 1120 301 1443 643
Fragments arranged by size:
1443 1120 643 301
SfcI C'TryA_G
Cuts at: 0 210 241 1741 2586 3507
Size: 210 31 1500 845 921
Fragments arranged by size:
1500 921 845 210 31
SfoI GGC'GCC
Cuts at: 0 2619 3507
Size: 2619 888
SmaI CCC'GGG
Cuts at: 0 1819 3507
Size: 1819 1688
SphI G_CATG'C
Cuts at: 0 1875 3507
Size: 1875 1632
StyI C'CwwG_G
Cuts at: 0 821 2027 2657 3507
Size: 821 1206 630 850
Fragments arranged by size:
1206 850 821 630
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 409 418 571 1324 1798 1848 2904
Size: 132 277 9 153 753 474 50 1056
Cuts at: 2904 3183 3507
Size: 279 324
Fragments arranged by size:
1056 753 474 324 279 277 153 132
50 9
TatI w'GTAC_w
Cuts at: 0 3233 3296 3380 3407 3507
Size: 3233 63 84 27 100
Fragments arranged by size:
3233 100 84 63 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 104 136 2929 2961 3507
Size: 104 32 2793 32 546
Fragments arranged by size:
2793 546 104 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1423 1615 3502 3507
Size: 1423 192 1887 5
Fragments arranged by size:
1887 1423 192 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 727 2664 2742 3507
Size: 141 586 1937 78 765
Fragments arranged by size:
1937 765 586 141 78
XmaI C'CCGG_G
Cuts at: 0 1817 3507
Size: 1817 1690
ZraI GAC'GTC
Cuts at: 0 2649 3099 3507
Size: 2649 450 408
Fragments arranged by size:
2649 450 408
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI
PsiI PspOMI PstI PvuII SacI SanDI SbfI SexAI
SfcI SfoI SmaI SphI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AflII AgeI AhdI AlfI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglI BlpI
BmtI BplI BpuEI BsaBI BsiWI BsmI BspEI BspHI
BssHII BstEII BstZ17I BtgZI CspCI EagI EarI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
NaeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII
SalI SapI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI