Restriction map for
pFN21AE1503
(Linear) (Six-base) MAPSORT of: KIEE1503.seq Check: 7085 from: 1 to: 771
KIEE1503.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AatII G_ACGT'C
Cuts at: 0 735 771
Size: 735 36
AclI AA'CG_TT
Cuts at: 0 290 771
Size: 290 481
AhdI GACnn_n'nnGTC
Cuts at: 0 731 771
Size: 731 40
AscI GG'CGCG_CC
Cuts at: 0 164 771
Size: 164 607
AvaI C'yCGr_G
Cuts at: 0 153 380 771
Size: 153 227 391
Fragments arranged by size:
391 227 153
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 320 353 771
Size: 320 33 418
Fragments arranged by size:
418 320 33
BamHI G'GATC_C
Cuts at: 0 192 771
Size: 192 579
BanI G'GyrC_C
Cuts at: 0 31 313 771
Size: 31 282 458
Fragments arranged by size:
458 282 31
BbeI G_GCGC'C
Cuts at: 0 35 771
Size: 35 736
BbsI GAAGACnn'nnnn_
Cuts at: 0 177 439 771
Size: 177 262 332
Fragments arranged by size:
332 262 177
BbvCI CC'TCA_GC
Cuts at: 0 477 771
Size: 477 294
BlpI GC'TnA_GC
Cuts at: 0 104 771
Size: 104 667
Bme1580I G_kGCm'C
Cuts at: 0 316 771
Size: 316 455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 380 530 771
Size: 367 13 150 241
Fragments arranged by size:
367 241 150 13
Bpu10I CC'TnA_GC
Cuts at: 0 477 771
Size: 477 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 356 418 712 771
Size: 356 62 294 59
Fragments arranged by size:
356 294 62 59
BsaAI yAC'GTr
Cuts at: 0 57 771
Size: 57 714
BsaBI GATnn'nnATC
Cuts at: 0 197 771
Size: 197 574
BsaHI Gr'CG_yC
Cuts at: 0 32 732 771
Size: 32 700 39
Fragments arranged by size:
700 39 32
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 465 771
Size: 364 101 306
Fragments arranged by size:
364 306 101
BseYI C'CCAG_C
Cuts at: 0 281 771
Size: 281 490
BsiHKAI G_wGCw'C
Cuts at: 0 305 771
Size: 305 466
BsmI GAATG_Cn'
Cuts at: 0 242 771
Size: 242 529
BsmBI CGTCTCn'nnnn_
Cuts at: 0 739 771
Size: 739 32
Bsp1286I G_dGCh'C
Cuts at: 0 305 316 771
Size: 305 11 455
Fragments arranged by size:
455 305 11
BsrBI CCG'CTC
Cuts at: 0 108 771
Size: 108 663
BsrDI GCAATG_nn'
Cuts at: 0 227 303 710 771
Size: 227 76 407 61
Fragments arranged by size:
407 227 76 61
BssHII G'CGCG_C
Cuts at: 0 50 79 87 164 771
Size: 50 29 8 77 607
Fragments arranged by size:
607 77 50 29 8
BssSI C'ACGA_G
Cuts at: 0 590 771
Size: 590 181
BstYI r'GATC_y
Cuts at: 0 192 771
Size: 192 579
Bsu36I CC'TnA_GG
Cuts at: 0 323 771
Size: 323 448
BtgI C'CryG_G
Cuts at: 0 15 92 159 366 771
Size: 15 77 67 207 405
Fragments arranged by size:
405 207 77 67 15
EaeI y'GGCC_r
Cuts at: 0 516 546 586 605 693 771
Size: 516 30 40 19 88 78
Fragments arranged by size:
516 88 78 40 30 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 380 530 771
Size: 367 13 150 241
Fragments arranged by size:
367 241 150 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 175 632 771
Size: 175 457 139
Fragments arranged by size:
457 175 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 771
Size: 201 570
HaeII r_GCGC'y
Cuts at: 0 35 77 771
Size: 35 42 694
Fragments arranged by size:
694 42 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 717 749 771
Size: 717 32 22
Fragments arranged by size:
717 32 22
KasI G'GCGC_C
Cuts at: 0 31 771
Size: 31 740
MscI TGG'CCA
Cuts at: 0 588 607 771
Size: 588 19 164
Fragments arranged by size:
588 164 19
MspA1I CmG'CkG
Cuts at: 0 161 771
Size: 161 610
NarI GG'CG_CC
Cuts at: 0 32 771
Size: 32 739
PfoI T'CCnGG_A
Cuts at: 0 188 771
Size: 188 583
PmlI CAC'GTG
Cuts at: 0 57 771
Size: 57 714
SacII CC_GC'GG
Cuts at: 0 162 771
Size: 162 609
SfoI GGC'GCC
Cuts at: 0 33 771
Size: 33 738
SmaI CCC'GGG
Cuts at: 0 155 382 771
Size: 155 227 389
Fragments arranged by size:
389 227 155
SrfI GCCC'GGGC
Cuts at: 0 382 771
Size: 382 389
StyI C'CwwG_G
Cuts at: 0 176 771
Size: 176 595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 289 489 615 771
Size: 289 200 126 156
Fragments arranged by size:
289 200 156 126
XmaI C'CCGG_G
Cuts at: 0 153 380 771
Size: 153 227 391
Fragments arranged by size:
391 227 153
ZraI GAC'GTC
Cuts at: 0 733 771
Size: 733 38
Enzymes that do cut:
AatII AclI AhdI AscI AvaI BaeI BamHI BanI
BbeI BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BsrBI BsrDI BssHII BssSI BstYI Bsu36I BtgI
EaeI Eco57MI EcoNI FalI HaeII Hin4I KasI MscI
MspA1I NarI PfoI PmlI SacII SfoI SmaI SrfI
StyI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvrII BanII BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BpuEI BsaWI BsaXI
BsgI BsiEI BsiWI BspEI BspHI BspMI BsrFI BsrGI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI