Restriction map for
pFN21AE1501
(Linear) (Six-base) MAPSORT of: KIEE1501.seq Check: 6216 from: 1 to: 849
KIEE1501.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AatII G_ACGT'C
Cuts at: 0 59 791 849
Size: 59 732 58
Fragments arranged by size:
732 59 58
AleI CACnn'nnGTG
Cuts at: 0 796 849
Size: 796 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 849
Size: 295 554
AvaI C'yCGr_G
Cuts at: 0 435 498 849
Size: 435 63 351
Fragments arranged by size:
435 351 63
BanI G'GyrC_C
Cuts at: 0 241 460 849
Size: 241 219 389
Fragments arranged by size:
389 241 219
BanII G_rGCy'C
Cuts at: 0 96 151 841 849
Size: 96 55 690 8
Fragments arranged by size:
690 96 55 8
BglI GCCn_nnn'nGGC
Cuts at: 0 239 775 849
Size: 239 536 74
Fragments arranged by size:
536 239 74
Bme1580I G_kGCm'C
Cuts at: 0 217 849
Size: 217 632
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 136 609 849
Size: 27 109 473 240
Fragments arranged by size:
473 240 109 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 54 221 318 414 547 849
Size: 32 22 167 97 96 133 302
Fragments arranged by size:
302 167 133 97 96 32 22
Bpu10I CC'TnA_GC
Cuts at: 0 268 849
Size: 268 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 849
Size: 81 768
BsaHI Gr'CG_yC
Cuts at: 0 56 788 849
Size: 56 732 61
Fragments arranged by size:
732 61 56
BsaWI w'CCGG_w
Cuts at: 0 783 849
Size: 783 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 412 442 495 525 849
Size: 121 30 261 30 53 30 324
Fragments arranged by size:
324 261 121 53 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 17 823 849
Size: 17 806 26
Fragments arranged by size:
806 26 17
BseYI C'CCAG_C
Cuts at: 0 108 300 580 849
Size: 108 192 280 269
Fragments arranged by size:
280 269 192 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 469 573 849
Size: 189 280 104 276
Fragments arranged by size:
280 276 189 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 720 849
Size: 720 129
Bsp1286I G_dGCh'C
Cuts at: 0 96 151 217 841 849
Size: 96 55 66 624 8
Fragments arranged by size:
624 96 66 55 8
BspEI T'CCGG_A
Cuts at: 0 783 849
Size: 783 66
BsrDI GCAATG_nn'
Cuts at: 0 248 849
Size: 248 601
BssHII G'CGCG_C
Cuts at: 0 315 849
Size: 315 534
BstXI CCAn_nnnn'nTGG
Cuts at: 0 262 849
Size: 262 587
BstYI r'GATC_y
Cuts at: 0 37 849
Size: 37 812
BtgI C'CryG_G
Cuts at: 0 305 794 803 849
Size: 305 489 9 46
Fragments arranged by size:
489 305 46 9
BtsI GCAGTG_nn'
Cuts at: 0 289 399 849
Size: 289 110 450
Fragments arranged by size:
450 289 110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 744 779 849
Size: 744 35 70
Fragments arranged by size:
744 70 35
DraIII CAC_nnn'GTG
Cuts at: 0 221 482 849
Size: 221 261 367
Fragments arranged by size:
367 261 221
EaeI y'GGCC_r
Cuts at: 0 800 849
Size: 800 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 54 221 318 414 547 849
Size: 32 22 167 97 96 133 302
Fragments arranged by size:
302 167 133 97 96 32 22
EcoO109I rG'GnC_Cy
Cuts at: 0 22 503 521 768 777 849
Size: 22 481 18 247 9 72
Fragments arranged by size:
481 247 72 22 18 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 495 527 849
Size: 495 32 322
Fragments arranged by size:
495 322 32
MslI CAynn'nnrTG
Cuts at: 0 260 670 796 849
Size: 260 410 126 53
Fragments arranged by size:
410 260 126 53
MspA1I CmG'CkG
Cuts at: 0 557 726 849
Size: 557 169 123
Fragments arranged by size:
557 169 123
NcoI C'CATG_G
Cuts at: 0 305 849
Size: 305 544
NdeI CA'TA_TG
Cuts at: 0 667 849
Size: 667 182
PfoI T'CCnGG_A
Cuts at: 0 9 334 386 849
Size: 9 325 52 463
Fragments arranged by size:
463 325 52 9
PpuMI rG'GwC_Cy
Cuts at: 0 503 521 849
Size: 503 18 328
Fragments arranged by size:
503 328 18
PstI C_TGCA'G
Cuts at: 0 284 364 776 849
Size: 284 80 412 73
Fragments arranged by size:
412 284 80 73
PvuII CAG'CTG
Cuts at: 0 557 849
Size: 557 292
SanDI GG'GwC_CC
Cuts at: 0 521 849
Size: 521 328
SbfI CC_TGCA'GG
Cuts at: 0 776 849
Size: 776 73
SfcI C'TryA_G
Cuts at: 0 280 360 772 849
Size: 280 80 412 77
Fragments arranged by size:
412 280 80 77
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 775 849
Size: 775 74
SmaI CCC'GGG
Cuts at: 0 437 500 849
Size: 437 63 349
Fragments arranged by size:
437 349 63
SmlI C'TyrA_G
Cuts at: 0 60 849
Size: 60 789
SrfI GCCC'GGGC
Cuts at: 0 437 849
Size: 437 412
StuI AGG'CCT
Cuts at: 0 196 849
Size: 196 653
StyI C'CwwG_G
Cuts at: 0 305 688 849
Size: 305 383 161
Fragments arranged by size:
383 305 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 466 498 849
Size: 466 32 351
Fragments arranged by size:
466 351 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 801 849
Size: 522 279 48
Fragments arranged by size:
522 279 48
XmaI C'CCGG_G
Cuts at: 0 435 498 849
Size: 435 63 351
Fragments arranged by size:
435 351 63
ZraI GAC'GTC
Cuts at: 0 57 789 849
Size: 57 732 60
Fragments arranged by size:
732 60 57
Enzymes that do cut:
AatII AleI AlfI AvaI BanI BanII BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsmBI Bsp1286I BspEI BsrDI BssHII BstXI
BstYI BtgI BtsI CspCI DraIII EaeI Eco57MI EcoO109I
Hin4I MslI MspA1I NcoI NdeI PfoI PpuMI PstI
PvuII SanDI SbfI SfcI SfiI SmaI SmlI SrfI
StuI StyI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BsaI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI
BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
TaqII TatI Tth111I XbaI XhoI XmnI