Restriction map for
pFN21AE1500
(Linear) (Six-base) MAPSORT of: KIEE1500.seq Check: 4898 from: 1 to: 2301
KIEE1500.orf
With 182 enzymes: SgfI *
August 26, 2010 17:36 ..
AclI AA'CG_TT
Cuts at: 0 1365 2301
Size: 1365 936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1423 2301
Size: 34 1389 878
Fragments arranged by size:
1389 878 34
AflII C'TTAA_G
Cuts at: 0 675 2301
Size: 675 1626
AflIII A'CryG_T
Cuts at: 0 945 2097 2301
Size: 945 1152 204
Fragments arranged by size:
1152 945 204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1204 1220 1346 2301
Size: 1204 16 126 955
Fragments arranged by size:
1204 955 126 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 785 817 1774 1806 2301
Size: 785 32 957 32 495
Fragments arranged by size:
957 785 495 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 1539 2301
Size: 180 1359 762
Fragments arranged by size:
1359 762 180
ApaI G_GGCC'C
Cuts at: 0 1723 2301
Size: 1723 578
ApoI r'AATT_y
Cuts at: 0 313 987 2110 2301
Size: 313 674 1123 191
Fragments arranged by size:
1123 674 313 191
AvaI C'yCGr_G
Cuts at: 0 1061 1767 2301
Size: 1061 706 534
Fragments arranged by size:
1061 706 534
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 190 223 2281 2301
Size: 190 33 2058 20
Fragments arranged by size:
2058 190 33 20
BamHI G'GATC_C
Cuts at: 0 71 2301
Size: 71 2230
BanI G'GyrC_C
Cuts at: 0 2031 2301
Size: 2031 270
BanII G_rGCy'C
Cuts at: 0 803 1160 1723 1940 1988 2301
Size: 803 357 563 217 48 313
Fragments arranged by size:
803 563 357 313 217 48
BbeI G_GCGC'C
Cuts at: 0 2035 2301
Size: 2035 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 875 1951 2301
Size: 875 1076 350
Fragments arranged by size:
1076 875 350
BbvCI CC'TCA_GC
Cuts at: 0 1708 2301
Size: 1708 593
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1758 1792 2301
Size: 1758 34 509
Fragments arranged by size:
1758 509 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 990 2282 2301
Size: 990 1292 19
Fragments arranged by size:
1292 990 19
BglI GCCn_nnn'nGGC
Cuts at: 0 358 2301
Size: 358 1943
BglII A'GATC_T
Cuts at: 0 162 2301
Size: 162 2139
BlpI GC'TnA_GC
Cuts at: 0 980 2301
Size: 980 1321
Bme1580I G_kGCm'C
Cuts at: 0 1723 2301
Size: 1723 578
BmrI ACTGGGnnnn_n'
Cuts at: 0 447 2301
Size: 447 1854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1075 1096 1231 1285 1339 1477 1549
Size: 138 937 21 135 54 54 138 72
Cuts at: 1549 1570 1612 1666 1912 1954 2233 2301
Size: 21 42 54 246 42 279 68
Fragments arranged by size:
937 279 246 138 138 135 72 68
54 54 54 42 42 21 21
Bpu10I CC'TnA_GC
Cuts at: 0 1037 1708 2301
Size: 1037 671 593
Fragments arranged by size:
1037 671 593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1433 1648 2185 2301
Size: 842 591 215 537 116
Fragments arranged by size:
842 591 537 215 116
BsaAI yAC'GTr
Cuts at: 0 948 1761 2301
Size: 948 813 540
Fragments arranged by size:
948 813 540
BsaHI Gr'CG_yC
Cuts at: 0 2032 2301
Size: 2032 269
BsaWI w'CCGG_w
Cuts at: 0 1940 2301
Size: 1940 361
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 785 815 1204 1234 2301
Size: 785 30 389 30 1067
Fragments arranged by size:
1067 785 389 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 1114 1474 1687 2200 2203 2301
Size: 655 459 360 213 513 3 98
Fragments arranged by size:
655 513 459 360 213 98 3
BseYI C'CCAG_C
Cuts at: 0 173 2301
Size: 173 2128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1210 2301
Size: 779 431 1091
Fragments arranged by size:
1091 779 431
BsiEI CG_ry'CG
Cuts at: 0 2269 2301
Size: 2269 32
BsiHKAI G_wGCw'C
Cuts at: 0 803 1160 1940 1988 2301
Size: 803 357 780 48 313
Fragments arranged by size:
803 780 357 313 48
BsmI GAATG_Cn'
Cuts at: 0 1277 1351 2301
Size: 1277 74 950
Fragments arranged by size:
1277 950 74
Bsp1286I G_dGCh'C
Cuts at: 0 803 1160 1723 1940 1988 2301
Size: 803 357 563 217 48 313
Fragments arranged by size:
803 563 357 313 217 48
BspEI T'CCGG_A
Cuts at: 0 1940 2301
Size: 1940 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1712 2301
Size: 1712 589
BsrDI GCAATG_nn'
Cuts at: 0 1723 2301
Size: 1723 578
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1086 1929 2301
Size: 1086 843 372
Fragments arranged by size:
1086 843 372
BstBI TT'CG_AA
Cuts at: 0 1991 2301
Size: 1991 310
BstYI r'GATC_y
Cuts at: 0 71 162 1842 2301
Size: 71 91 1680 459
Fragments arranged by size:
1680 459 91 71
Bsu36I CC'TnA_GG
Cuts at: 0 1654 2301
Size: 1654 647
BtgI C'CryG_G
Cuts at: 0 76 1442 1838 2293 2301
Size: 76 1366 396 455 8
Fragments arranged by size:
1366 455 396 76 8
BtsI GCAGTG_nn'
Cuts at: 0 845 1688 2301
Size: 845 843 613
Fragments arranged by size:
845 843 613
DraI TTT'AAA
Cuts at: 0 566 2108 2301
Size: 566 1542 193
Fragments arranged by size:
1542 566 193
EaeI y'GGCC_r
Cuts at: 0 1439 2266 2301
Size: 1439 827 35
Fragments arranged by size:
1439 827 35
EagI C'GGCC_G
Cuts at: 0 2266 2301
Size: 2266 35
EarI CTCTTCn'nnn_
Cuts at: 0 26 716 907 1475 1993 2301
Size: 26 690 191 568 518 308
Fragments arranged by size:
690 568 518 308 191 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1684 2301
Size: 1684 617
EcoICRI GAG'CTC
Cuts at: 0 801 1158 1938 1986 2301
Size: 801 357 780 48 315
Fragments arranged by size:
801 780 357 315 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 138 1075 1096 1231 1285 1339 1423
Size: 34 104 937 21 135 54 54 84
Cuts at: 1423 1477 1549 1570 1612 1666 1912 1954 2233
Size: 54 72 21 42 54 246 42 279
Cuts at: 2233 2301
Size: 68
Fragments arranged by size:
937 279 246 135 104 84 72 68
54 54 54 54 42 42 34 21
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1459 2301
Size: 1459 842
EcoO109I rG'GnC_Cy
Cuts at: 0 1200 1650 1658 1701 1719 1782 2301
Size: 1200 450 8 43 18 63 519
Fragments arranged by size:
1200 519 450 63 43 18 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 286 2301
Size: 286 2015
HaeII r_GCGC'y
Cuts at: 0 1243 2035 2301
Size: 1243 792 266
Fragments arranged by size:
1243 792 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 468 500 995 1027 2301
Size: 154 32 282 32 495 32 1274
Fragments arranged by size:
1274 495 282 154 32 32 32
HindIII A'AGCT_T
Cuts at: 0 268 2301
Size: 268 2033
KasI G'GCGC_C
Cuts at: 0 2031 2301
Size: 2031 270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1825 2301
Size: 1134 691 476
Fragments arranged by size:
1134 691 476
MscI TGG'CCA
Cuts at: 0 1441 2301
Size: 1441 860
MspA1I CmG'CkG
Cuts at: 0 1282 1590 1632 1926 2301
Size: 1282 308 42 294 375
Fragments arranged by size:
1282 375 308 294 42
NarI GG'CG_CC
Cuts at: 0 2032 2301
Size: 2032 269
NcoI C'CATG_G
Cuts at: 0 1442 1838 2293 2301
Size: 1442 396 455 8
Fragments arranged by size:
1442 455 396 8
NruI TCG'CGA
Cuts at: 0 2126 2301
Size: 2126 175
NspI r_CATG'y
Cuts at: 0 561 2101 2199 2301
Size: 561 1540 98 102
Fragments arranged by size:
1540 561 102 98
PciI A'CATG_T
Cuts at: 0 2097 2301
Size: 2097 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 1257 2301
Size: 1257 1044
PfoI T'CCnGG_A
Cuts at: 0 1997 2301
Size: 1997 304
PmlI CAC'GTG
Cuts at: 0 948 1761 2301
Size: 948 813 540
Fragments arranged by size:
948 813 540
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 785 817 2301
Size: 785 32 1484
Fragments arranged by size:
1484 785 32
PpuMI rG'GwC_Cy
Cuts at: 0 1658 1701 1782 2301
Size: 1658 43 81 519
Fragments arranged by size:
1658 519 81 43
PsiI TTA'TAA
Cuts at: 0 2149 2301
Size: 2149 152
PspOMI G'GGCC_C
Cuts at: 0 1719 2301
Size: 1719 582
PspXI vC'TCGA_Gb
Cuts at: 0 1767 2301
Size: 1767 534
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1355 1387 2301
Size: 1355 32 914
Fragments arranged by size:
1355 914 32
PstI C_TGCA'G
Cuts at: 0 1622 1637 1683 1809 1955 2301
Size: 1622 15 46 126 146 346
Fragments arranged by size:
1622 346 146 126 46 15
PvuII CAG'CTG
Cuts at: 0 1282 1590 1632 2301
Size: 1282 308 42 669
Fragments arranged by size:
1282 669 308 42
SacI G_AGCT'C
Cuts at: 0 803 1160 1940 1988 2301
Size: 803 357 780 48 313
Fragments arranged by size:
803 780 357 313 48
SapI GCTCTTCn'nnn_
Cuts at: 0 716 1993 2301
Size: 716 1277 308
Fragments arranged by size:
1277 716 308
SfcI C'TryA_G
Cuts at: 0 1618 1633 1679 1805 1855 1951 2301
Size: 1618 15 46 126 50 96 350
Fragments arranged by size:
1618 350 126 96 50 46 15
SfoI GGC'GCC
Cuts at: 0 2033 2301
Size: 2033 268
SmlI C'TyrA_G
Cuts at: 0 675 857 1448 1663 1767 2200 2301
Size: 675 182 591 215 104 433 101
Fragments arranged by size:
675 591 433 215 182 104 101
SspI AAT'ATT
Cuts at: 0 441 2301
Size: 441 1860
StuI AGG'CCT
Cuts at: 0 1093 2301
Size: 1093 1208
StyI C'CwwG_G
Cuts at: 0 905 1442 1838 2252 2293 2301
Size: 905 537 396 414 41 8
Fragments arranged by size:
905 537 414 396 41 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1249 2301
Size: 1249 1052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1050 2301
Size: 1050 1251
XhoI C'TCGA_G
Cuts at: 0 1767 2301
Size: 1767 534
XmnI GAAnn'nnTTC
Cuts at: 0 487 1559 2301
Size: 487 1072 742
Fragments arranged by size:
1072 742 487
Enzymes that do cut:
AclI AcuI AflII AflIII AlfI AloI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BstAPI BstBI BstYI Bsu36I BtgI BtsI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HindIII KasI MmeI MscI MspA1I
NarI NcoI NruI NspI PciI PflMI PfoI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII
SacI SapI SfcI SfoI SmlI SspI StuI StyI
TaqII XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvrII BclI BfrBI BmgBI
BmtI BplI BsaI BsaBI BsiWI BsmBI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraIII DrdI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MslI NaeI
NdeI NgoMIV NheI NotI NsiI PacI PasI PmeI
PshAI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TatI TstI Tth111I XbaI XmaI ZraI