Restriction map for
pFN21AE1493
(Linear) (Six-base) MAPSORT of: KIEE1493.seq Check: 7086 from: 1 to: 876
KIEE1493.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 876
Size: 493 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 876
Size: 323 553
AlwNI CAG_nnn'CTG
Cuts at: 0 682 876
Size: 682 194
ApoI r'AATT_y
Cuts at: 0 76 829 876
Size: 76 753 47
Fragments arranged by size:
753 76 47
BanI G'GyrC_C
Cuts at: 0 271 876
Size: 271 605
BanII G_rGCy'C
Cuts at: 0 41 177 750 876
Size: 41 136 573 126
Fragments arranged by size:
573 136 126 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 876
Size: 287 589
BbvCI CC'TCA_GC
Cuts at: 0 252 876
Size: 252 624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 151 480 514 876
Size: 117 34 329 34 362
Fragments arranged by size:
362 329 117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 234 876
Size: 234 642
BclI T'GATC_A
Cuts at: 0 800 876
Size: 800 76
BglI GCCn_nnn'nGGC
Cuts at: 0 673 876
Size: 673 203
Bme1580I G_kGCm'C
Cuts at: 0 726 876
Size: 726 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 748 876
Size: 256 492 128
Fragments arranged by size:
492 256 128
Bpu10I CC'TnA_GC
Cuts at: 0 252 876
Size: 252 624
BsaAI yAC'GTr
Cuts at: 0 441 876
Size: 441 435
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 164 194 876
Size: 164 30 682
Fragments arranged by size:
682 164 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 495 573 876
Size: 13 482 78 303
Fragments arranged by size:
482 303 78 13
BseYI C'CCAG_C
Cuts at: 0 514 876
Size: 514 362
BsiHKAI G_wGCw'C
Cuts at: 0 750 876
Size: 750 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 507 876
Size: 507 369
Bsp1286I G_dGCh'C
Cuts at: 0 41 177 726 750 876
Size: 41 136 549 24 126
Fragments arranged by size:
549 136 126 41 24
BspHI T'CATG_A
Cuts at: 0 387 876
Size: 387 489
BsrDI GCAATG_nn'
Cuts at: 0 263 489 876
Size: 263 226 387
Fragments arranged by size:
387 263 226
BstYI r'GATC_y
Cuts at: 0 821 876
Size: 821 55
BtgI C'CryG_G
Cuts at: 0 275 876
Size: 275 601
BtsI GCAGTG_nn'
Cuts at: 0 228 876
Size: 228 648
DraIII CAC_nnn'GTG
Cuts at: 0 405 876
Size: 405 471
DrdI GACnn_nn'nnGTC
Cuts at: 0 195 876
Size: 195 681
EarI CTCTTCn'nnn_
Cuts at: 0 46 135 800 876
Size: 46 89 665 76
Fragments arranged by size:
665 89 76 46
EcoICRI GAG'CTC
Cuts at: 0 748 876
Size: 748 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 493 748 876
Size: 256 237 255 128
Fragments arranged by size:
256 255 237 128
EcoRI G'AATT_C
Cuts at: 0 76 876
Size: 76 800
FspI TGC'GCA
Cuts at: 0 409 876
Size: 409 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 792 824 876
Size: 138 32 622 32 52
Fragments arranged by size:
622 138 52 32 32
HincII GTy'rAC
Cuts at: 0 426 876
Size: 426 450
NcoI C'CATG_G
Cuts at: 0 275 876
Size: 275 601
NdeI CA'TA_TG
Cuts at: 0 695 876
Size: 695 181
NspI r_CATG'y
Cuts at: 0 345 876
Size: 345 531
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 234 876
Size: 183 51 642
Fragments arranged by size:
642 183 51
PfoI T'CCnGG_A
Cuts at: 0 824 876
Size: 824 52
SacI G_AGCT'C
Cuts at: 0 750 876
Size: 750 126
SapI GCTCTTCn'nnn_
Cuts at: 0 46 876
Size: 46 830
SfcI C'TryA_G
Cuts at: 0 211 452 876
Size: 211 241 424
Fragments arranged by size:
424 241 211
SspI AAT'ATT
Cuts at: 0 601 876
Size: 601 275
StyI C'CwwG_G
Cuts at: 0 96 275 876
Size: 96 179 601
Fragments arranged by size:
601 179 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 164 196 876
Size: 92 32 40 32 680
Fragments arranged by size:
680 92 40 32 32
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI Bme1580I BpmI Bpu10I BsaAI
BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrDI
BstYI BtgI BtsI DraIII DrdI EarI EcoICRI Eco57MI
EcoRI FspI Hin4I HincII NcoI NdeI NspI PflMI
PfoI SacI SapI SfcI SspI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EaeI
EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI