Restriction map for
pFN21AE1492
(Linear) (Six-base) MAPSORT of: KIEE1492.seq Check: 6087 from: 1 to: 2538
KIEE1492.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
Acc65I G'GTAC_C
Cuts at: 0 798 2538
Size: 798 1740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 1361 1388 2538
Size: 23 1338 27 1150
Fragments arranged by size:
1338 1150 27 23
AflII C'TTAA_G
Cuts at: 0 301 2538
Size: 301 2237
AflIII A'CryG_T
Cuts at: 0 650 1078 2538
Size: 650 428 1460
Fragments arranged by size:
1460 650 428
AhdI GACnn_n'nnGTC
Cuts at: 0 678 1986 2538
Size: 678 1308 552
Fragments arranged by size:
1308 678 552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 729 1903 2354 2538
Size: 729 1174 451 184
Fragments arranged by size:
1174 729 451 184
AlwNI CAG_nnn'CTG
Cuts at: 0 1033 1572 2538
Size: 1033 539 966
Fragments arranged by size:
1033 966 539
ApoI r'AATT_y
Cuts at: 0 181 1444 1774 2041 2538
Size: 181 1263 330 267 497
Fragments arranged by size:
1263 497 330 267 181
AvaI C'yCGr_G
Cuts at: 0 344 2538
Size: 344 2194
AvrII C'CTAG_G
Cuts at: 0 1429 2538
Size: 1429 1109
BanI G'GyrC_C
Cuts at: 0 798 2538
Size: 798 1740
BanII G_rGCy'C
Cuts at: 0 1870 2381 2538
Size: 1870 511 157
Fragments arranged by size:
1870 511 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 1327 2538
Size: 1327 1211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 301 335 2538
Size: 301 34 2203
Fragments arranged by size:
2203 301 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 81 2538
Size: 81 2457
BclI T'GATC_A
Cuts at: 0 585 1710 2538
Size: 585 1125 828
Fragments arranged by size:
1125 828 585
BfrBI ATG'CAT
Cuts at: 0 2065 2538
Size: 2065 473
BglII A'GATC_T
Cuts at: 0 268 2538
Size: 268 2270
BlpI GC'TnA_GC
Cuts at: 0 2296 2538
Size: 2296 242
BmrI ACTGGGnnnn_n'
Cuts at: 0 2440 2538
Size: 2440 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1773 1889 2538
Size: 1773 116 649
Fragments arranged by size:
1773 649 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 2456 2538
Size: 1856 600 82
Fragments arranged by size:
1856 600 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 2538
Size: 766 1772
BsaI GGTCTCn'nnnn_
Cuts at: 0 419 1622 2538
Size: 419 1203 916
Fragments arranged by size:
1203 916 419
BsaAI yAC'GTr
Cuts at: 0 1669 2538
Size: 1669 869
BsaBI GATnn'nnATC
Cuts at: 0 790 914 2538
Size: 790 124 1624
Fragments arranged by size:
1624 790 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2140 2170 2538
Size: 2140 30 368
Fragments arranged by size:
2140 368 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1984 2538
Size: 1984 554
BseYI C'CCAG_C
Cuts at: 0 937 2538
Size: 937 1601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1286 1505 2538
Size: 627 659 219 1033
Fragments arranged by size:
1033 659 627 219
BsiHKAI G_wGCw'C
Cuts at: 0 2086 2381 2538
Size: 2086 295 157
Fragments arranged by size:
2086 295 157
Bsp1286I G_dGCh'C
Cuts at: 0 1870 2086 2381 2538
Size: 1870 216 295 157
Fragments arranged by size:
1870 295 216 157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 63 1502 2286 2538
Size: 63 1439 784 252
Fragments arranged by size:
1439 784 252 63
BsrDI GCAATG_nn'
Cuts at: 0 1885 2538
Size: 1885 653
BsrFI r'CCGG_y
Cuts at: 0 2307 2538
Size: 2307 231
BssSI C'ACGA_G
Cuts at: 0 433 2538
Size: 433 2105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2035 2538
Size: 2035 503
BstYI r'GATC_y
Cuts at: 0 268 516 2538
Size: 268 248 2022
Fragments arranged by size:
2022 268 248
BtgI C'CryG_G
Cuts at: 0 383 2538
Size: 383 2155
BtsI GCAGTG_nn'
Cuts at: 0 685 1031 2047 2538
Size: 685 346 1016 491
Fragments arranged by size:
1016 685 491 346
ClaI AT'CG_AT
Cuts at: 0 1013 2538
Size: 1013 1525
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1169 1204 1784 1819 2538
Size: 1169 35 580 35 719
Fragments arranged by size:
1169 719 580 35 35
DraI TTT'AAA
Cuts at: 0 551 2400 2538
Size: 551 1849 138
Fragments arranged by size:
1849 551 138
DraIII CAC_nnn'GTG
Cuts at: 0 691 2538
Size: 691 1847
EaeI y'GGCC_r
Cuts at: 0 2511 2538
Size: 2511 27
EarI CTCTTCn'nnn_
Cuts at: 0 270 629 971 1742 2317 2477 2538
Size: 270 359 342 771 575 160 61
Fragments arranged by size:
771 575 359 342 270 160 61
EcoICRI GAG'CTC
Cuts at: 0 2379 2538
Size: 2379 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 1361 1388 1856 2456 2538
Size: 23 1338 27 468 600 82
Fragments arranged by size:
1338 600 468 82 27 23
EcoO109I rG'GnC_Cy
Cuts at: 0 2275 2538
Size: 2275 263
EcoRI G'AATT_C
Cuts at: 0 2041 2538
Size: 2041 497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 313 2538
Size: 313 2225
FspI TGC'GCA
Cuts at: 0 458 2538
Size: 458 2080
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 764 796 1531 1563 1735 1767
Size: 664 32 68 32 735 32 172 32
Cuts at: 1767 1972 2004 2140 2172 2253 2285 2316 2348
Size: 205 32 136 32 81 32 31 32
Cuts at: 2348 2444 2476 2538
Size: 96 32 62
Fragments arranged by size:
735 664 205 172 136 96 81 68
62 32 32 32 32 32 32 32
32 32 31
HincII GTy'rAC
Cuts at: 0 1653 2538
Size: 1653 885
HindIII A'AGCT_T
Cuts at: 0 35 2071 2416 2538
Size: 35 2036 345 122
Fragments arranged by size:
2036 345 122 35
KpnI G_GTAC'C
Cuts at: 0 802 2538
Size: 802 1736
MfeI C'AATT_G
Cuts at: 0 1165 2538
Size: 1165 1373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2487 2538
Size: 2487 51
MslI CAynn'nnrTG
Cuts at: 0 52 1036 2336 2538
Size: 52 984 1300 202
Fragments arranged by size:
1300 984 202 52
NaeI GCC'GGC
Cuts at: 0 2309 2538
Size: 2309 229
NcoI C'CATG_G
Cuts at: 0 383 2538
Size: 383 2155
NgoMIV G'CCGG_C
Cuts at: 0 2307 2538
Size: 2307 231
NsiI A_TGCA'T
Cuts at: 0 2067 2538
Size: 2067 471
NspI r_CATG'y
Cuts at: 0 382 654 716 1082 2015 2538
Size: 382 272 62 366 933 523
Fragments arranged by size:
933 523 382 366 272 62
PciI A'CATG_T
Cuts at: 0 650 1078 2538
Size: 650 428 1460
Fragments arranged by size:
1460 650 428
PflMI CCAn_nnn'nTGG
Cuts at: 0 1572 2538
Size: 1572 966
PpuMI rG'GwC_Cy
Cuts at: 0 2275 2538
Size: 2275 263
PshAI GACnn'nnGTC
Cuts at: 0 1137 2538
Size: 1137 1401
PstI C_TGCA'G
Cuts at: 0 2054 2241 2538
Size: 2054 187 297
Fragments arranged by size:
2054 297 187
SacI G_AGCT'C
Cuts at: 0 2381 2538
Size: 2381 157
SapI GCTCTTCn'nnn_
Cuts at: 0 971 2538
Size: 971 1567
ScaI AGT'ACT
Cuts at: 0 1185 1212 2538
Size: 1185 27 1326
Fragments arranged by size:
1326 1185 27
SexAI A'CCwGG_T
Cuts at: 0 1385 2538
Size: 1385 1153
SfcI C'TryA_G
Cuts at: 0 2050 2237 2538
Size: 2050 187 301
Fragments arranged by size:
2050 301 187
SmlI C'TyrA_G
Cuts at: 0 301 344 781 2538
Size: 301 43 437 1757
Fragments arranged by size:
1757 437 301 43
SphI G_CATG'C
Cuts at: 0 716 2538
Size: 716 1822
SspI AAT'ATT
Cuts at: 0 2124 2531 2538
Size: 2124 407 7
Fragments arranged by size:
2124 407 7
StyI C'CwwG_G
Cuts at: 0 383 1231 1429 2538
Size: 383 848 198 1109
Fragments arranged by size:
1109 848 383 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1812 2538
Size: 1812 726
TatI w'GTAC_w
Cuts at: 0 238 1183 1210 2538
Size: 238 945 27 1328
Fragments arranged by size:
1328 945 238 27
XbaI T'CTAG_A
Cuts at: 0 271 2538
Size: 271 2267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 2538
Size: 568 1970
XhoI C'TCGA_G
Cuts at: 0 344 2538
Size: 344 2194
XmnI GAAnn'nnTTC
Cuts at: 0 2417 2538
Size: 2417 121
Enzymes that do cut:
Acc65I AcuI AflII AflIII AhdI AlfI AlwNI ApoI
AvaI AvrII BanI BanII BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BssSI BstXI BstYI BtgI BtsI
ClaI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI Hin4I HincII HindIII KpnI
MfeI MmeI MslI NaeI NcoI NgoMIV NsiI NspI
PciI PflMI PpuMI PshAI PstI SacI SapI ScaI
SexAI SfcI SmlI SphI SspI StyI TaqII TatI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbvCI BglI Bme1580I BmgBI BmtI Bpu10I BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI DrdI
EagI EciI EcoNI EcoRV FseI FspAI HaeII HpaI
KasI MluI MscI MspA1I NarI NdeI NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XmaI ZraI