Restriction map for
pFN21AE1490
(Linear) (Six-base) MAPSORT of: KIEE1490.seq Check: 6798 from: 1 to: 2010
KIEE1490.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 20 2010
Size: 20 1990
AccI GT'mk_AC
Cuts at: 0 1964 2010
Size: 1964 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1182 1505 1708 2010
Size: 151 1031 323 203 302
Fragments arranged by size:
1031 323 302 203 151
AfeI AGC'GCT
Cuts at: 0 947 1089 1628 1928 2010
Size: 947 142 539 300 82
Fragments arranged by size:
947 539 300 142 82
AflIII A'CryG_T
Cuts at: 0 665 1798 2010
Size: 665 1133 212
Fragments arranged by size:
1133 665 212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1253 1285 2010
Size: 1253 32 725
Fragments arranged by size:
1253 725 32
ApaI G_GGCC'C
Cuts at: 0 1388 2010
Size: 1388 622
ApoI r'AATT_y
Cuts at: 0 937 2010
Size: 937 1073
AvaI C'yCGr_G
Cuts at: 0 583 603 970 1006 1380 1822 2010
Size: 583 20 367 36 374 442 188
Fragments arranged by size:
583 442 374 367 188 36 20
BamHI G'GATC_C
Cuts at: 0 1644 1826 2010
Size: 1644 182 184
Fragments arranged by size:
1644 184 182
BanI G'GyrC_C
Cuts at: 0 1208 2010
Size: 1208 802
BanII G_rGCy'C
Cuts at: 0 86 899 1007 1085 1174 1388 1463 2010
Size: 86 813 108 78 89 214 75 547
Fragments arranged by size:
813 547 214 108 89 86 78 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 1493 2010
Size: 1493 517
BbvCI CC'TCA_GC
Cuts at: 0 1696 2010
Size: 1696 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1387 1421 1803 1837 2010
Size: 1387 34 382 34 173
Fragments arranged by size:
1387 382 173 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 595 1383 1568 2010
Size: 595 788 185 442
Fragments arranged by size:
788 595 442 185
BglII A'GATC_T
Cuts at: 0 1431 1511 2010
Size: 1431 80 499
Fragments arranged by size:
1431 499 80
BlpI GC'TnA_GC
Cuts at: 0 154 1216 2010
Size: 154 1062 794
Fragments arranged by size:
1062 794 154
Bme1580I G_kGCm'C
Cuts at: 0 1388 1919 2010
Size: 1388 531 91
Fragments arranged by size:
1388 531 91
BmgBI CAC'GTC
Cuts at: 0 1801 2010
Size: 1801 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1032 2010
Size: 1032 978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 780 1272 1343 1536 1867 1960 2010
Size: 385 395 492 71 193 331 93 50
Fragments arranged by size:
492 395 385 331 193 93 71 50
Bpu10I CC'TnA_GC
Cuts at: 0 149 863 1079 1630 1696 2010
Size: 149 714 216 551 66 314
Fragments arranged by size:
714 551 314 216 149 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 2010
Size: 1081 929
BsaI GGTCTCn'nnnn_
Cuts at: 0 1404 2010
Size: 1404 606
BsaAI yAC'GTr
Cuts at: 0 1914 2010
Size: 1914 96
BsaBI GATnn'nnATC
Cuts at: 0 1777 2010
Size: 1777 233
BsaWI w'CCGG_w
Cuts at: 0 44 1727 2010
Size: 44 1683 283
Fragments arranged by size:
1683 283 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1253 1283 1867 1897 2010
Size: 1253 30 584 30 113
Fragments arranged by size:
1253 584 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 879 987 1888 2010
Size: 879 108 901 122
Fragments arranged by size:
901 879 122 108
BseYI C'CCAG_C
Cuts at: 0 85 143 399 821 975 1084 1153 1237
Size: 85 58 256 422 154 109 69 84
Cuts at: 1237 1303 1456 1558 1623 1649 2010
Size: 66 153 102 65 26 361
Fragments arranged by size:
422 361 256 154 153 109 102 85
84 69 66 65 58 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 2010
Size: 215 1795
BsiEI CG_ry'CG
Cuts at: 0 931 935 1187 1454 2010
Size: 931 4 252 267 556
Fragments arranged by size:
931 556 267 252 4
BsiHKAI G_wGCw'C
Cuts at: 0 164 1174 2010
Size: 164 1010 836
Fragments arranged by size:
1010 836 164
BsmI GAATG_Cn'
Cuts at: 0 559 2010
Size: 559 1451
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1936 2010
Size: 1936 74
Bsp1286I G_dGCh'C
Cuts at: 0 86 164 899 1007 1085 1174 1388 1463
Size: 86 78 735 108 78 89 214 75
Cuts at: 1463 1919 2010
Size: 456 91
Fragments arranged by size:
735 456 214 108 91 89 86 78
78 75
BspEI T'CCGG_A
Cuts at: 0 44 1727 2010
Size: 44 1683 283
Fragments arranged by size:
1683 283 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 101 1488 2010
Size: 20 81 1387 522
Fragments arranged by size:
1387 522 81 20
BsrBI CCG'CTC
Cuts at: 0 748 1215 1448 2010
Size: 748 467 233 562
Fragments arranged by size:
748 562 467 233
BsrDI GCAATG_nn'
Cuts at: 0 1875 2010
Size: 1875 135
BsrFI r'CCGG_y
Cuts at: 0 486 589 930 1192 2010
Size: 486 103 341 262 818
Fragments arranged by size:
818 486 341 262 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1347 2010
Size: 1347 663
BstYI r'GATC_y
Cuts at: 0 1431 1511 1644 1826 1841 2010
Size: 1431 80 133 182 15 169
Fragments arranged by size:
1431 182 169 133 80 15
Bsu36I CC'TnA_GG
Cuts at: 0 221 257 293 329 437 692 2010
Size: 221 36 36 36 108 255 1318
Fragments arranged by size:
1318 255 221 108 36 36 36
BtgI C'CryG_G
Cuts at: 0 23 925 1187 1388 1670 2010
Size: 23 902 262 201 282 340
Fragments arranged by size:
902 340 282 262 201 23
BtsI GCAGTG_nn'
Cuts at: 0 203 1174 2010
Size: 203 971 836
Fragments arranged by size:
971 836 203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1643 1678 2010
Size: 1643 35 332
Fragments arranged by size:
1643 332 35
EaeI y'GGCC_r
Cuts at: 0 928 932 1184 1190 1451 2010
Size: 928 4 252 6 261 559
Fragments arranged by size:
928 559 261 252 6 4
EagI C'GGCC_G
Cuts at: 0 928 932 1184 1451 2010
Size: 928 4 252 267 559
Fragments arranged by size:
928 559 267 252 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1335 2010
Size: 1335 675
EcoICRI GAG'CTC
Cuts at: 0 1172 2010
Size: 1172 838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 385 780 1182 1272 1343 1505 1536
Size: 151 234 395 402 90 71 162 31
Cuts at: 1536 1708 1867 1960 2010
Size: 172 159 93 50
Fragments arranged by size:
402 395 234 172 162 159 151 93
90 71 50 31
EcoO109I rG'GnC_Cy
Cuts at: 0 174 450 608 873 884 992 1384 1442
Size: 174 276 158 265 11 108 392 58
Cuts at: 1442 1500 1890 2010
Size: 58 390 120
Fragments arranged by size:
392 390 276 265 174 158 120 108
58 58 11
EcoRI G'AATT_C
Cuts at: 0 937 2010
Size: 937 1073
FseI GG_CCGG'CC
Cuts at: 0 934 2010
Size: 934 1076
FspI TGC'GCA
Cuts at: 0 650 2010
Size: 650 1360
HaeII r_GCGC'y
Cuts at: 0 803 949 1050 1091 1585 1630 1930 2010
Size: 803 146 101 41 494 45 300 80
Fragments arranged by size:
803 494 300 146 101 80 45 41
HincII GTy'rAC
Cuts at: 0 1965 2010
Size: 1965 45
MslI CAynn'nnrTG
Cuts at: 0 1771 2010
Size: 1771 239
MspA1I CmG'CkG
Cuts at: 0 180 399 831 927 1237 1250 1374 1456
Size: 180 219 432 96 310 13 124 82
Cuts at: 1456 1695 2010
Size: 239 315
Fragments arranged by size:
432 315 310 239 219 180 124 96
82 13
NaeI GCC'GGC
Cuts at: 0 488 932 1194 2010
Size: 488 444 262 816
Fragments arranged by size:
816 488 444 262
NcoI C'CATG_G
Cuts at: 0 23 1670 2010
Size: 23 1647 340
Fragments arranged by size:
1647 340 23
NgoMIV G'CCGG_C
Cuts at: 0 486 930 1192 2010
Size: 486 444 262 818
Fragments arranged by size:
818 486 444 262
PasI CC'CwG_GG
Cuts at: 0 1222 2001 2010
Size: 1222 779 9
Fragments arranged by size:
1222 779 9
PfoI T'CCnGG_A
Cuts at: 0 1469 1844 2010
Size: 1469 375 166
Fragments arranged by size:
1469 375 166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1253 1285 1708 1740 2010
Size: 994 32 227 32 423 32 270
Fragments arranged by size:
994 423 270 227 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 608 873 884 992 1442 1500 1890 2010
Size: 608 265 11 108 450 58 390 120
Fragments arranged by size:
608 450 390 265 120 108 58 11
PspOMI G'GGCC_C
Cuts at: 0 1384 2010
Size: 1384 626
PstI C_TGCA'G
Cuts at: 0 21 434 506 1040 1422 2010
Size: 21 413 72 534 382 588
Fragments arranged by size:
588 534 413 382 72 21
PvuII CAG'CTG
Cuts at: 0 399 1695 2010
Size: 399 1296 315
Fragments arranged by size:
1296 399 315
SacI G_AGCT'C
Cuts at: 0 1174 2010
Size: 1174 836
SacII CC_GC'GG
Cuts at: 0 928 2010
Size: 928 1082
SalI G'TCGA_C
Cuts at: 0 1963 2010
Size: 1963 47
SanDI GG'GwC_CC
Cuts at: 0 1890 2010
Size: 1890 120
SfcI C'TryA_G
Cuts at: 0 17 430 502 1036 1418 2010
Size: 17 413 72 534 382 592
Fragments arranged by size:
592 534 413 382 72 17
SmaI CCC'GGG
Cuts at: 0 1382 2010
Size: 1382 628
SmlI C'TyrA_G
Cuts at: 0 1096 2010
Size: 1096 914
StuI AGG'CCT
Cuts at: 0 70 463 817 1265 1295 1326 2010
Size: 70 393 354 448 30 31 684
Fragments arranged by size:
684 448 393 354 70 31 30
StyI C'CwwG_G
Cuts at: 0 23 418 1565 1670 2010
Size: 23 395 1147 105 340
Fragments arranged by size:
1147 395 340 105 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1490 1683 2010
Size: 1490 193 327
Fragments arranged by size:
1490 327 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 431 1867 1899 2010
Size: 399 32 1436 32 111
Fragments arranged by size:
1436 399 111 32 32
XmaI C'CCGG_G
Cuts at: 0 1380 2010
Size: 1380 630
XmnI GAAnn'nnTTC
Cuts at: 0 136 1735 2010
Size: 136 1599 275
Fragments arranged by size:
1599 275 136
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AloI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI
BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI CspCI
EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII HincII MslI MspA1I NaeI NcoI NgoMIV
PasI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SalI SanDI SfcI SmaI SmlI StuI StyI
TaqII TstI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BmrI BmtI BsaHI BsiWI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI DraI DraIII DrdI EarI EcoNI EcoRV FalI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XcmI XhoI ZraI