Restriction map for
pFN21AE1486
(Linear) (Six-base) MAPSORT of: KIEE1486.seq Check: 4380 from: 1 to: 2709
KIEE1486.orf
With 182 enzymes: SgfI *
July 3, 2012 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 108 1474 2709
Size: 108 1366 1235
Fragments arranged by size:
1366 1235 108
AatII G_ACGT'C
Cuts at: 0 188 2709
Size: 188 2521
AccI GT'mk_AC
Cuts at: 0 1814 1991 2411 2687 2709
Size: 1814 177 420 276 22
Fragments arranged by size:
1814 420 276 177 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1336 1523 1611 1663 1918 2709
Size: 495 841 187 88 52 255 791
Fragments arranged by size:
841 791 495 255 187 88 52
AfeI AGC'GCT
Cuts at: 0 2000 2709
Size: 2000 709
AgeI A'CCGG_T
Cuts at: 0 2651 2709
Size: 2651 58
AleI CACnn'nnGTG
Cuts at: 0 2272 2709
Size: 2272 437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1654 2709
Size: 1654 1055
AlwNI CAG_nnn'CTG
Cuts at: 0 1089 1204 2709
Size: 1089 115 1505
Fragments arranged by size:
1505 1089 115
ApaI G_GGCC'C
Cuts at: 0 700 1198 2709
Size: 700 498 1511
Fragments arranged by size:
1511 700 498
ApaLI G'TGCA_C
Cuts at: 0 2380 2709
Size: 2380 329
AvaI C'yCGr_G
Cuts at: 0 321 662 1572 2709
Size: 321 341 910 1137
Fragments arranged by size:
1137 910 341 321
BanI G'GyrC_C
Cuts at: 0 519 852 1839 1912 2162 2709
Size: 519 333 987 73 250 547
Fragments arranged by size:
987 547 519 333 250 73
BanII G_rGCy'C
Cuts at: 0 275 700 1198 1285 1579 2331 2709
Size: 275 425 498 87 294 752 378
Fragments arranged by size:
752 498 425 378 294 275 87
BbeI G_GCGC'C
Cuts at: 0 856 2709
Size: 856 1853
BbsI GAAGACnn'nnnn_
Cuts at: 0 188 1556 1771 2590 2709
Size: 188 1368 215 819 119
Fragments arranged by size:
1368 819 215 188 119
BbvCI CC'TCA_GC
Cuts at: 0 142 485 1670 2709
Size: 142 343 1185 1039
Fragments arranged by size:
1185 1039 343 142
BglII A'GATC_T
Cuts at: 0 1919 1958 2709
Size: 1919 39 751
Fragments arranged by size:
1919 751 39
Bme1580I G_kGCm'C
Cuts at: 0 442 700 1198 1625 2165 2384 2709
Size: 442 258 498 427 540 219 325
Fragments arranged by size:
540 498 442 427 325 258 219
BmgBI CAC'GTC
Cuts at: 0 2415 2709
Size: 2415 294
BmrI ACTGGGnnnn_n'
Cuts at: 0 209 859 919 1165 1403 2266 2673 2709
Size: 209 650 60 246 238 863 407 36
Fragments arranged by size:
863 650 407 246 238 209 60 36
BmtI G_CTAG'C
Cuts at: 0 400 2709
Size: 400 2309
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1587 2709
Size: 1587 1122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 271 412 456 1493 2709
Size: 215 56 141 44 1037 1216
Fragments arranged by size:
1216 1037 215 141 56 44
Bpu10I CC'TnA_GC
Cuts at: 0 142 485 1670 2570 2709
Size: 142 343 1185 900 139
Fragments arranged by size:
1185 900 343 142 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1744 2709
Size: 1744 965
BsaI GGTCTCn'nnnn_
Cuts at: 0 1558 2347 2709
Size: 1558 789 362
Fragments arranged by size:
1558 789 362
BsaAI yAC'GTr
Cuts at: 0 1305 2379 2709
Size: 1305 1074 330
Fragments arranged by size:
1305 1074 330
BsaHI Gr'CG_yC
Cuts at: 0 185 853 875 2709
Size: 185 668 22 1834
Fragments arranged by size:
1834 668 185 22
BsaWI w'CCGG_w
Cuts at: 0 2517 2651 2709
Size: 2517 134 58
Fragments arranged by size:
2517 134 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 766 796 1360 1390 2453 2483
Size: 213 30 523 30 564 30 1063 30
Cuts at: 2483 2621 2651 2709
Size: 138 30 58
Fragments arranged by size:
1063 564 523 213 138 58 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 934 955 976 997 1018 1039 2365
Size: 46 888 21 21 21 21 21 1326
Cuts at: 2365 2709
Size: 344
Fragments arranged by size:
1326 888 344 46 21 21 21 21
21
BseYI C'CCAG_C
Cuts at: 0 276 700 2709
Size: 276 424 2009
Fragments arranged by size:
2009 424 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 790 1349 2194 2709
Size: 157 633 559 845 515
Fragments arranged by size:
845 633 559 515 157
BsiEI CG_ry'CG
Cuts at: 0 1860 2709
Size: 1860 849
BsiHKAI G_wGCw'C
Cuts at: 0 2384 2420 2709
Size: 2384 36 289
Fragments arranged by size:
2384 289 36
BsmI GAATG_Cn'
Cuts at: 0 326 2709
Size: 326 2383
Bsp1286I G_dGCh'C
Cuts at: 0 275 442 700 1198 1285 1579 1625 2165
Size: 275 167 258 498 87 294 46 540
Cuts at: 2165 2331 2384 2420 2709
Size: 166 53 36 289
Fragments arranged by size:
540 498 294 289 275 258 167 166
87 53 46 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 108 1105 1474 2709
Size: 108 997 369 1235
Fragments arranged by size:
1235 997 369 108
BsrDI GCAATG_nn'
Cuts at: 0 34 280 921 1638 2059 2709
Size: 34 246 641 717 421 650
Fragments arranged by size:
717 650 641 421 246 34
BsrFI r'CCGG_y
Cuts at: 0 2651 2709
Size: 2651 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1234 2566 2709
Size: 1234 1332 143
Fragments arranged by size:
1332 1234 143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2274 2364 2709
Size: 2274 90 345
Fragments arranged by size:
2274 345 90
BstYI r'GATC_y
Cuts at: 0 1919 1958 2709
Size: 1919 39 751
Fragments arranged by size:
1919 751 39
BtgI C'CryG_G
Cuts at: 0 176 1097 1685 1739 2126 2709
Size: 176 921 588 54 387 583
Fragments arranged by size:
921 588 583 387 176 54
BtsI GCAGTG_nn'
Cuts at: 0 1202 1515 1603 2074 2560 2709
Size: 1202 313 88 471 486 149
Fragments arranged by size:
1202 486 471 313 149 88
ClaI AT'CG_AT
Cuts at: 0 424 2709
Size: 424 2285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1083 1118 2709
Size: 1083 35 1591
Fragments arranged by size:
1591 1083 35
EaeI y'GGCC_r
Cuts at: 0 1091 2709
Size: 1091 1618
EarI CTCTTCn'nnn_
Cuts at: 0 28 733 773 1151 1312 1409 2709
Size: 28 705 40 378 161 97 1300
Fragments arranged by size:
1300 705 378 161 97 40 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2405 2709
Size: 2405 304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 271 412 456 495 1336 1493 1523
Size: 215 56 141 44 39 841 157 30
Cuts at: 1523 1611 1663 1918 2709
Size: 88 52 255 791
Fragments arranged by size:
841 791 255 215 157 141 88 56
52 44 39 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 513 2709
Size: 513 2196
EcoO109I rG'GnC_Cy
Cuts at: 0 696 697 2315 2709
Size: 696 1 1618 394
Fragments arranged by size:
1618 696 394 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1511 2709
Size: 1511 1198
FspI TGC'GCA
Cuts at: 0 2209 2709
Size: 2209 500
HaeII r_GCGC'y
Cuts at: 0 55 856 1884 2002 2709
Size: 55 801 1028 118 707
Fragments arranged by size:
1028 801 707 118 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2453 2485 2709
Size: 2453 32 224
Fragments arranged by size:
2453 224 32
HincII GTy'rAC
Cuts at: 0 1815 2145 2709
Size: 1815 330 564
Fragments arranged by size:
1815 564 330
KasI G'GCGC_C
Cuts at: 0 852 2709
Size: 852 1857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1823 2607 2614 2709
Size: 1823 784 7 95
Fragments arranged by size:
1823 784 95 7
MscI TGG'CCA
Cuts at: 0 1093 2709
Size: 1093 1616
MslI CAynn'nnrTG
Cuts at: 0 346 1441 1468 2125 2272 2709
Size: 346 1095 27 657 147 437
Fragments arranged by size:
1095 657 437 346 147 27
MspA1I CmG'CkG
Cuts at: 0 141 484 1231 2613 2709
Size: 141 343 747 1382 96
Fragments arranged by size:
1382 747 343 141 96
NarI GG'CG_CC
Cuts at: 0 853 2709
Size: 853 1856
NcoI C'CATG_G
Cuts at: 0 1685 2709
Size: 1685 1024
NheI G'CTAG_C
Cuts at: 0 396 2709
Size: 396 2313
NspI r_CATG'y
Cuts at: 0 673 1473 2709
Size: 673 800 1236
Fragments arranged by size:
1236 800 673
PasI CC'CwG_GG
Cuts at: 0 1580 2709
Size: 1580 1129
PflMI CCAn_nnn'nTGG
Cuts at: 0 759 2709
Size: 759 1950
PfoI T'CCnGG_A
Cuts at: 0 1509 2709
Size: 1509 1200
PshAI GACnn'nnGTC
Cuts at: 0 188 2709
Size: 188 2521
PspOMI G'GGCC_C
Cuts at: 0 696 1194 2709
Size: 696 498 1515
Fragments arranged by size:
1515 696 498
PspXI vC'TCGA_Gb
Cuts at: 0 1572 2709
Size: 1572 1137
PstI C_TGCA'G
Cuts at: 0 270 2709
Size: 270 2439
PvuII CAG'CTG
Cuts at: 0 141 484 1231 2613 2709
Size: 141 343 747 1382 96
Fragments arranged by size:
1382 747 343 141 96
RsrII CG'GwC_CG
Cuts at: 0 1425 2709
Size: 1425 1284
SalI G'TCGA_C
Cuts at: 0 1813 2709
Size: 1813 896
SapI GCTCTTCn'nnn_
Cuts at: 0 28 733 1312 2709
Size: 28 705 579 1397
Fragments arranged by size:
1397 705 579 28
ScaI AGT'ACT
Cuts at: 0 1617 2709
Size: 1617 1092
SexAI A'CCwGG_T
Cuts at: 0 2147 2709
Size: 2147 562
SfcI C'TryA_G
Cuts at: 0 266 2709
Size: 266 2443
SfoI GGC'GCC
Cuts at: 0 854 2709
Size: 854 1855
SmlI C'TyrA_G
Cuts at: 0 1572 1759 2709
Size: 1572 187 950
Fragments arranged by size:
1572 950 187
StuI AGG'CCT
Cuts at: 0 1268 2672 2709
Size: 1268 1404 37
Fragments arranged by size:
1404 1268 37
StyI C'CwwG_G
Cuts at: 0 29 523 1143 1340 1685 2709
Size: 29 494 620 197 345 1024
Fragments arranged by size:
1024 620 494 345 197 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 1846 1960 2709
Size: 311 1535 114 749
Fragments arranged by size:
1535 749 311 114
TatI w'GTAC_w
Cuts at: 0 382 462 1615 2709
Size: 382 80 1153 1094
Fragments arranged by size:
1153 1094 382 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 213 245 1360 1392 2621 2653 2709
Size: 213 32 1115 32 1229 32 56
Fragments arranged by size:
1229 1115 213 56 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2440 2709
Size: 2440 269
XbaI T'CTAG_A
Cuts at: 0 432 2709
Size: 432 2277
XhoI C'TCGA_G
Cuts at: 0 1572 2709
Size: 1572 1137
XmnI GAAnn'nnTTC
Cuts at: 0 2020 2471 2709
Size: 2020 451 238
Fragments arranged by size:
2020 451 238
ZraI GAC'GTC
Cuts at: 0 186 2709
Size: 186 2523
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtsI ClaI CspCI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NarI NcoI NheI NspI PasI PflMI PfoI PshAI
PspOMI PspXI PstI PvuII RsrII SalI SapI ScaI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BlpI BsaBI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PsiI PsrI PvuI SacI SacII
SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XcmI XmaI