Restriction map for
pFN21AE1484
(Linear) (Six-base) MAPSORT of: KIEE1484.seq Check: 1056 from: 1 to: 1965
KIEE1484.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AatII G_ACGT'C
Cuts at: 0 1960 1965
Size: 1960 5
AccI GT'mk_AC
Cuts at: 0 754 1965
Size: 754 1211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 266 820 1965
Size: 110 156 554 1145
Fragments arranged by size:
1145 554 156 110
AflIII A'CryG_T
Cuts at: 0 1086 1965
Size: 1086 879
AleI CACnn'nnGTG
Cuts at: 0 1087 1965
Size: 1087 878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 1965
Size: 640 1325
AlwNI CAG_nnn'CTG
Cuts at: 0 469 1506 1965
Size: 469 1037 459
Fragments arranged by size:
1037 469 459
ApaLI G'TGCA_C
Cuts at: 0 967 1965
Size: 967 998
ApoI r'AATT_y
Cuts at: 0 220 572 828 904 1301 1568 1965
Size: 220 352 256 76 397 267 397
Fragments arranged by size:
397 397 352 267 256 220 76
BanI G'GyrC_C
Cuts at: 0 531 1846 1965
Size: 531 1315 119
Fragments arranged by size:
1315 531 119
BanII G_rGCy'C
Cuts at: 0 140 1487 1965
Size: 140 1347 478
Fragments arranged by size:
1347 478 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 1676 1965
Size: 773 903 289
Fragments arranged by size:
903 773 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1913 1947 1965
Size: 1913 34 18
Fragments arranged by size:
1913 34 18
BglI GCCn_nnn'nGGC
Cuts at: 0 1836 1965
Size: 1836 129
BglII A'GATC_T
Cuts at: 0 1242 1965
Size: 1242 723
BlpI GC'TnA_GC
Cuts at: 0 1036 1965
Size: 1036 929
Bme1580I G_kGCm'C
Cuts at: 0 971 1965
Size: 971 994
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 1965
Size: 29 1936
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1629 1632 1965
Size: 1629 3 333
Fragments arranged by size:
1629 333 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1765 1965
Size: 299 1466 200
Fragments arranged by size:
1466 299 200
Bpu10I CC'TnA_GC
Cuts at: 0 1451 1965
Size: 1451 514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1897 1965
Size: 1897 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 809 1171 1965
Size: 809 362 794
Fragments arranged by size:
809 794 362
BsaAI yAC'GTr
Cuts at: 0 657 1965
Size: 657 1308
BsaHI Gr'CG_yC
Cuts at: 0 1871 1957 1965
Size: 1871 86 8
Fragments arranged by size:
1871 86 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1819 1849 1965
Size: 1819 30 116
Fragments arranged by size:
1819 116 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 1213 1495 1605 1621 1660 1663 1750
Size: 763 450 282 110 16 39 3 87
Cuts at: 1750 1840 1965
Size: 90 125
Fragments arranged by size:
763 450 282 125 110 90 87 39
16 3
BseYI C'CCAG_C
Cuts at: 0 82 343 1936 1965
Size: 82 261 1593 29
Fragments arranged by size:
1593 261 82 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1069 1786 1965
Size: 919 150 717 179
Fragments arranged by size:
919 717 179 150
BsiEI CG_ry'CG
Cuts at: 0 1593 1965
Size: 1593 372
BsiHKAI G_wGCw'C
Cuts at: 0 971 1037 1487 1965
Size: 971 66 450 478
Fragments arranged by size:
971 478 450 66
BsmI GAATG_Cn'
Cuts at: 0 617 1965
Size: 617 1348
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1859 1948 1965
Size: 1859 89 17
Fragments arranged by size:
1859 89 17
Bsp1286I G_dGCh'C
Cuts at: 0 140 971 1037 1487 1965
Size: 140 831 66 450 478
Fragments arranged by size:
831 478 450 140 66
BspHI T'CATG_A
Cuts at: 0 1731 1965
Size: 1731 234
BsrDI GCAATG_nn'
Cuts at: 0 983 1965
Size: 983 982
BsrFI r'CCGG_y
Cuts at: 0 1511 1920 1965
Size: 1511 409 45
Fragments arranged by size:
1511 409 45
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 1506 1965
Size: 810 696 459
Fragments arranged by size:
810 696 459
BstYI r'GATC_y
Cuts at: 0 1242 1463 1965
Size: 1242 221 502
Fragments arranged by size:
1242 502 221
BtgI C'CryG_G
Cuts at: 0 881 1493 1965
Size: 881 612 472
Fragments arranged by size:
881 612 472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1155 1916 1965
Size: 1155 761 49
Fragments arranged by size:
1155 761 49
DraI TTT'AAA
Cuts at: 0 375 1965
Size: 375 1590
EaeI y'GGCC_r
Cuts at: 0 1154 1490 1590 1965
Size: 1154 336 100 375
Fragments arranged by size:
1154 375 336 100
EagI C'GGCC_G
Cuts at: 0 1590 1965
Size: 1590 375
EarI CTCTTCn'nnn_
Cuts at: 0 86 304 857 1403 1529 1685 1965
Size: 86 218 553 546 126 156 280
Fragments arranged by size:
553 546 280 218 156 126 86
EcoICRI GAG'CTC
Cuts at: 0 1485 1965
Size: 1485 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 266 299 820 1765 1965
Size: 110 156 33 521 945 200
Fragments arranged by size:
945 521 200 156 110 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1450 1965
Size: 1450 515
EcoRI G'AATT_C
Cuts at: 0 572 904 1965
Size: 572 332 1061
Fragments arranged by size:
1061 572 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1965
Size: 363 1602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 433 465 1044 1076 1119 1151
Size: 166 32 235 32 579 32 43 32
Cuts at: 1151 1365 1397 1856 1888 1965
Size: 214 32 459 32 77
Fragments arranged by size:
579 459 235 214 166 77 43 32
32 32 32 32 32
MscI TGG'CCA
Cuts at: 0 1156 1492 1965
Size: 1156 336 473
Fragments arranged by size:
1156 473 336
MslI CAynn'nnrTG
Cuts at: 0 1087 1965
Size: 1087 878
MspA1I CmG'CkG
Cuts at: 0 65 469 1770 1965
Size: 65 404 1301 195
Fragments arranged by size:
1301 404 195 65
NaeI GCC'GGC
Cuts at: 0 1513 1922 1965
Size: 1513 409 43
Fragments arranged by size:
1513 409 43
NcoI C'CATG_G
Cuts at: 0 1493 1965
Size: 1493 472
NgoMIV G'CCGG_C
Cuts at: 0 1511 1920 1965
Size: 1511 409 45
Fragments arranged by size:
1511 409 45
NspI r_CATG'y
Cuts at: 0 1090 1965
Size: 1090 875
PciI A'CATG_T
Cuts at: 0 1086 1965
Size: 1086 879
PfoI T'CCnGG_A
Cuts at: 0 114 1965
Size: 114 1851
PsiI TTA'TAA
Cuts at: 0 1435 1965
Size: 1435 530
PstI C_TGCA'G
Cuts at: 0 1250 1658 1965
Size: 1250 408 307
Fragments arranged by size:
1250 408 307
PvuII CAG'CTG
Cuts at: 0 469 1770 1965
Size: 469 1301 195
Fragments arranged by size:
1301 469 195
SacI G_AGCT'C
Cuts at: 0 1487 1965
Size: 1487 478
SapI GCTCTTCn'nnn_
Cuts at: 0 1685 1965
Size: 1685 280
ScaI AGT'ACT
Cuts at: 0 1612 1965
Size: 1612 353
SfcI C'TryA_G
Cuts at: 0 690 785 1028 1246 1654 1965
Size: 690 95 243 218 408 311
Fragments arranged by size:
690 408 311 243 218 95
SmlI C'TyrA_G
Cuts at: 0 1876 1965
Size: 1876 89
SnaBI TAC'GTA
Cuts at: 0 657 1965
Size: 657 1308
SspI AAT'ATT
Cuts at: 0 1440 1965
Size: 1440 525
StyI C'CwwG_G
Cuts at: 0 317 1493 1796 1965
Size: 317 1176 303 169
Fragments arranged by size:
1176 317 303 169
TatI w'GTAC_w
Cuts at: 0 1293 1610 1965
Size: 1293 317 355
Fragments arranged by size:
1293 355 317
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1819 1851 1965
Size: 1819 32 114
Fragments arranged by size:
1819 114 32
Tth111I GACn'n_nGTC
Cuts at: 0 979 1870 1965
Size: 979 891 95
Fragments arranged by size:
979 891 95
ZraI GAC'GTC
Cuts at: 0 1958 1965
Size: 1958 7
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI BanI BanII BbsI BcgI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrDI BsrFI BstAPI BstYI BtgI
BtgZI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoRI FalI Hin4I MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PciI PfoI PsiI PstI PvuII SacI
SapI ScaI SfcI SmlI SnaBI SspI StyI TatI
TstI Tth111I ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI BsaBI
BsaWI BsiWI BspEI BspMI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraIII DrdI EciI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI StuI SwaI TaqII XbaI XcmI XhoI XmaI
XmnI