Restriction map for
pFN21AE1481
(Linear) (Six-base) MAPSORT of: KIEE1481.seq Check: 7455 from: 1 to: 1029
KIEE1481.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 350 1029
Size: 350 679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 625 655 985 1029
Size: 467 158 30 330 44
Fragments arranged by size:
467 330 158 44 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 388 1029
Size: 388 641
AlwNI CAG_nnn'CTG
Cuts at: 0 963 1029
Size: 963 66
ApoI r'AATT_y
Cuts at: 0 913 1029
Size: 913 116
AvaI C'yCGr_G
Cuts at: 0 523 1029
Size: 523 506
BanII G_rGCy'C
Cuts at: 0 995 1029
Size: 995 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 1029
Size: 29 1000
BbvCI CC'TCA_GC
Cuts at: 0 938 1029
Size: 938 91
BclI T'GATC_A
Cuts at: 0 533 1029
Size: 533 496
Bme1580I G_kGCm'C
Cuts at: 0 384 1029
Size: 384 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 705 1029
Size: 241 464 324
Fragments arranged by size:
464 324 241
Bpu10I CC'TnA_GC
Cuts at: 0 599 938 989 1029
Size: 599 339 51 40
Fragments arranged by size:
599 339 51 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1029
Size: 337 692
BsaI GGTCTCn'nnnn_
Cuts at: 0 794 1029
Size: 794 235
BsaBI GATnn'nnATC
Cuts at: 0 365 1029
Size: 365 664
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 937 940 1029
Size: 94 843 3 89
Fragments arranged by size:
843 94 89 3
BseYI C'CCAG_C
Cuts at: 0 995 1029
Size: 995 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 901 1029
Size: 526 375 128
Fragments arranged by size:
526 375 128
Bsp1286I G_dGCh'C
Cuts at: 0 384 995 1029
Size: 384 611 34
Fragments arranged by size:
611 384 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 413 812 1029
Size: 350 63 399 217
Fragments arranged by size:
399 350 217 63
BsrDI GCAATG_nn'
Cuts at: 0 198 736 1029
Size: 198 538 293
Fragments arranged by size:
538 293 198
BstYI r'GATC_y
Cuts at: 0 657 1029
Size: 657 372
Bsu36I CC'TnA_GG
Cuts at: 0 119 754 1029
Size: 119 635 275
Fragments arranged by size:
635 275 119
DraIII CAC_nnn'GTG
Cuts at: 0 507 549 1029
Size: 507 42 480
Fragments arranged by size:
507 480 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 315 1029
Size: 315 714
EaeI y'GGCC_r
Cuts at: 0 770 788 1029
Size: 770 18 241
Fragments arranged by size:
770 241 18
EarI CTCTTCn'nnn_
Cuts at: 0 53 104 1029
Size: 53 51 925
Fragments arranged by size:
925 53 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 467 625 655 705 985 1029
Size: 241 226 158 30 50 280 44
Fragments arranged by size:
280 241 226 158 50 44 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 907 1029
Size: 145 762 122
Fragments arranged by size:
762 145 122
EcoO109I rG'GnC_Cy
Cuts at: 0 237 873 1029
Size: 237 636 156
Fragments arranged by size:
636 237 156
EcoRI G'AATT_C
Cuts at: 0 913 1029
Size: 913 116
HindIII A'AGCT_T
Cuts at: 0 793 1029
Size: 793 236
MscI TGG'CCA
Cuts at: 0 772 790 1029
Size: 772 18 239
Fragments arranged by size:
772 239 18
MslI CAynn'nnrTG
Cuts at: 0 209 502 1029
Size: 209 293 527
Fragments arranged by size:
527 293 209
MspA1I CmG'CkG
Cuts at: 0 570 1029
Size: 570 459
NspI r_CATG'y
Cuts at: 0 633 1029
Size: 633 396
PasI CC'CwG_GG
Cuts at: 0 582 1029
Size: 582 447
PpuMI rG'GwC_Cy
Cuts at: 0 237 1029
Size: 237 792
PsiI TTA'TAA
Cuts at: 0 428 1029
Size: 428 601
PstI C_TGCA'G
Cuts at: 0 410 1029
Size: 410 619
PvuII CAG'CTG
Cuts at: 0 570 1029
Size: 570 459
SfcI C'TryA_G
Cuts at: 0 93 406 1029
Size: 93 313 623
Fragments arranged by size:
623 313 93
SmlI C'TyrA_G
Cuts at: 0 352 1029
Size: 352 677
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 1029
Size: 352 677
XbaI T'CTAG_A
Cuts at: 0 18 1029
Size: 18 1011
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI BanII BbsI
BbvCI BclI Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI
BseRI BseYI BsgI Bsp1286I BspMI BsrDI BstYI Bsu36I
DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
HindIII MscI MslI MspA1I NspI PasI PpuMI PsiI
PstI PvuII SfcI SmlI TaqII XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BsaAI
BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI