Restriction map for
pFN21AE1478
(Linear) (Six-base) MAPSORT of: KIEE1478.seq Check: 4985 from: 1 to: 1521
KIEE1478.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 496 1082 1491 1521
Size: 496 586 409 30
Fragments arranged by size:
586 496 409 30
AccI GT'mk_AC
Cuts at: 0 368 1412 1521
Size: 368 1044 109
Fragments arranged by size:
1044 368 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1182 1521
Size: 1182 339
AflIII A'CryG_T
Cuts at: 0 1138 1521
Size: 1138 383
AleI CACnn'nnGTG
Cuts at: 0 220 550 1117 1141 1521
Size: 220 330 567 24 380
Fragments arranged by size:
567 380 330 220 24
AlwNI CAG_nnn'CTG
Cuts at: 0 915 1521
Size: 915 606
ApaI G_GGCC'C
Cuts at: 0 16 1521
Size: 16 1505
ApoI r'AATT_y
Cuts at: 0 780 1521
Size: 780 741
AvaI C'yCGr_G
Cuts at: 0 195 499 581 1061 1521
Size: 195 304 82 480 460
Fragments arranged by size:
480 460 304 195 82
BanI G'GyrC_C
Cuts at: 0 36 105 319 495 703 1521
Size: 36 69 214 176 208 818
Fragments arranged by size:
818 214 208 176 69 36
BanII G_rGCy'C
Cuts at: 0 16 317 1521
Size: 16 301 1204
Fragments arranged by size:
1204 301 16
BbeI G_GCGC'C
Cuts at: 0 40 109 1521
Size: 40 69 1412
Fragments arranged by size:
1412 69 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 273 444 978 1363 1521
Size: 273 171 534 385 158
Fragments arranged by size:
534 385 273 171 158
BbvCI CC'TCA_GC
Cuts at: 0 1396 1521
Size: 1396 125
BclI T'GATC_A
Cuts at: 0 629 800 1521
Size: 629 171 721
Fragments arranged by size:
721 629 171
BglI GCCn_nnn'nGGC
Cuts at: 0 1449 1521
Size: 1449 72
Bme1580I G_kGCm'C
Cuts at: 0 16 500 1521
Size: 16 484 1021
Fragments arranged by size:
1021 484 16
BmtI G_CTAG'C
Cuts at: 0 34 1521
Size: 34 1487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 1521
Size: 255 1266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1023 1441 1521
Size: 382 641 418 80
Fragments arranged by size:
641 418 382 80
Bpu10I CC'TnA_GC
Cuts at: 0 1268 1396 1521
Size: 1268 128 125
Fragments arranged by size:
1268 128 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 376 454 1294 1521
Size: 211 165 78 840 227
Fragments arranged by size:
840 227 211 165 78
BsaAI yAC'GTr
Cuts at: 0 867 1139 1521
Size: 867 272 382
Fragments arranged by size:
867 382 272
BsaHI Gr'CG_yC
Cuts at: 0 37 106 211 278 962 1521
Size: 37 69 105 67 684 559
Fragments arranged by size:
684 559 105 69 67 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1063 1093 1150 1165 1180 1195 1521
Size: 1063 30 57 15 15 15 326
Fragments arranged by size:
1063 326 57 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 517 1099 1444 1521
Size: 64 453 582 345 77
Fragments arranged by size:
582 453 345 77 64
BseYI C'CCAG_C
Cuts at: 0 639 1521
Size: 639 882
BsiEI CG_ry'CG
Cuts at: 0 47 551 1478 1521
Size: 47 504 927 43
Fragments arranged by size:
927 504 47 43
BsiHKAI G_wGCw'C
Cuts at: 0 227 317 1521
Size: 227 90 1204
Fragments arranged by size:
1204 227 90
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1350 1521
Size: 1350 171
Bsp1286I G_dGCh'C
Cuts at: 0 16 227 317 500 1521
Size: 16 211 90 183 1021
Fragments arranged by size:
1021 211 183 90 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 496 827 1082 1491 1521
Size: 473 23 331 255 409 30
Fragments arranged by size:
473 409 331 255 30 23
BsrBI CCG'CTC
Cuts at: 0 477 1278 1521
Size: 477 801 243
Fragments arranged by size:
801 477 243
BsrFI r'CCGG_y
Cuts at: 0 39 108 1521
Size: 39 69 1413
Fragments arranged by size:
1413 69 39
BssHII G'CGCG_C
Cuts at: 0 24 26 296 1521
Size: 24 2 270 1225
Fragments arranged by size:
1225 270 24 2
BssSI C'ACGA_G
Cuts at: 0 517 1129 1521
Size: 517 612 392
Fragments arranged by size:
612 517 392
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 519 615 1521
Size: 177 342 96 906
Fragments arranged by size:
906 342 177 96
BstYI r'GATC_y
Cuts at: 0 681 1521
Size: 681 840
BtgI C'CryG_G
Cuts at: 0 215 929 935 1364 1521
Size: 215 714 6 429 157
Fragments arranged by size:
714 429 215 157 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 413 1521
Size: 413 1108
EaeI y'GGCC_r
Cuts at: 0 44 80 167 548 827 932 1229 1521
Size: 44 36 87 381 279 105 297 292
Fragments arranged by size:
381 297 292 279 105 87 44 36
EagI C'GGCC_G
Cuts at: 0 44 548 1521
Size: 44 504 973
Fragments arranged by size:
973 504 44
EarI CTCTTCn'nnn_
Cuts at: 0 53 484 1051 1336 1521
Size: 53 431 567 285 185
Fragments arranged by size:
567 431 285 185 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 356 1521
Size: 356 1165
EcoICRI GAG'CTC
Cuts at: 0 315 1521
Size: 315 1206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1023 1182 1441 1521
Size: 382 641 159 259 80
Fragments arranged by size:
641 382 259 159 80
EcoO109I rG'GnC_Cy
Cuts at: 0 1022 1058 1521
Size: 1022 36 463
Fragments arranged by size:
1022 463 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 1194 1521
Size: 495 699 327
Fragments arranged by size:
699 495 327
HaeII r_GCGC'y
Cuts at: 0 40 109 256 1521
Size: 40 69 147 1265
Fragments arranged by size:
1265 147 69 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1521
Size: 1165 32 324
Fragments arranged by size:
1165 324 32
KasI G'GCGC_C
Cuts at: 0 36 105 1521
Size: 36 69 1416
Fragments arranged by size:
1416 69 36
MscI TGG'CCA
Cuts at: 0 169 829 1231 1521
Size: 169 660 402 290
Fragments arranged by size:
660 402 290 169
MslI CAynn'nnrTG
Cuts at: 0 175 205 220 550 1117 1141 1240 1288
Size: 175 30 15 330 567 24 99 48
Cuts at: 1288 1501 1521
Size: 213 20
Fragments arranged by size:
567 330 213 175 99 48 30 24
20 15
MspA1I CmG'CkG
Cuts at: 0 141 746 1400 1521
Size: 141 605 654 121
Fragments arranged by size:
654 605 141 121
NaeI GCC'GGC
Cuts at: 0 41 110 1521
Size: 41 69 1411
Fragments arranged by size:
1411 69 41
NarI GG'CG_CC
Cuts at: 0 37 106 1521
Size: 37 69 1415
Fragments arranged by size:
1415 69 37
NgoMIV G'CCGG_C
Cuts at: 0 39 108 1521
Size: 39 69 1413
Fragments arranged by size:
1413 69 39
NheI G'CTAG_C
Cuts at: 0 30 1521
Size: 30 1491
NspI r_CATG'y
Cuts at: 0 360 1521
Size: 360 1161
PfoI T'CCnGG_A
Cuts at: 0 587 970 1521
Size: 587 383 551
Fragments arranged by size:
587 551 383
PmlI CAC'GTG
Cuts at: 0 1139 1521
Size: 1139 382
PpuMI rG'GwC_Cy
Cuts at: 0 1022 1058 1521
Size: 1022 36 463
Fragments arranged by size:
1022 463 36
PspOMI G'GGCC_C
Cuts at: 0 12 1521
Size: 12 1509
PstI C_TGCA'G
Cuts at: 0 140 1521
Size: 140 1381
SacI G_AGCT'C
Cuts at: 0 317 1521
Size: 317 1204
SanDI GG'GwC_CC
Cuts at: 0 1022 1058 1521
Size: 1022 36 463
Fragments arranged by size:
1022 463 36
SapI GCTCTTCn'nnn_
Cuts at: 0 484 1051 1521
Size: 484 567 470
Fragments arranged by size:
567 484 470
SfcI C'TryA_G
Cuts at: 0 136 561 810 1521
Size: 136 425 249 711
Fragments arranged by size:
711 425 249 136
SfoI GGC'GCC
Cuts at: 0 38 107 1521
Size: 38 69 1414
Fragments arranged by size:
1414 69 38
SgrAI Cr'CCGG_yG
Cuts at: 0 39 1521
Size: 39 1482
SmaI CCC'GGG
Cuts at: 0 583 1063 1521
Size: 583 480 458
Fragments arranged by size:
583 480 458
SmlI C'TyrA_G
Cuts at: 0 226 391 469 1273 1521
Size: 226 165 78 804 248
Fragments arranged by size:
804 248 226 165 78
StyI C'CwwG_G
Cuts at: 0 1169 1521
Size: 1169 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1115 1521
Size: 1115 406
TatI w'GTAC_w
Cuts at: 0 434 1521
Size: 434 1087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 1521
Size: 1063 32 426
Fragments arranged by size:
1063 426 32
XbaI T'CTAG_A
Cuts at: 0 678 1521
Size: 678 843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 855 1521
Size: 714 141 666
Fragments arranged by size:
714 666 141
XmaI C'CCGG_G
Cuts at: 0 581 1061 1521
Size: 581 480 460
Fragments arranged by size:
581 480 460
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BclI BglI
Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI BtgZI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PfoI PmlI PpuMI PspOMI PstI
SacI SanDI SapI SfcI SfoI SgrAI SmaI SmlI
StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BsaI
BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XhoI XmnI ZraI