Restriction map for
pFN21AE1473
(Linear) (Six-base) MAPSORT of: KIEE1473.seq Check: 982 from: 1 to: 1143
KIEE1473.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1040 1143
Size: 421 619 103
Fragments arranged by size:
619 421 103
AflII C'TTAA_G
Cuts at: 0 244 1143
Size: 244 899
AflIII A'CryG_T
Cuts at: 0 1085 1087 1143
Size: 1085 2 56
Fragments arranged by size:
1085 56 2
ApoI r'AATT_y
Cuts at: 0 212 312 441 466 665 1143
Size: 212 100 129 25 199 478
Fragments arranged by size:
478 212 199 129 100 25
AvaI C'yCGr_G
Cuts at: 0 43 166 1143
Size: 43 123 977
Fragments arranged by size:
977 123 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 356 389 1143
Size: 356 33 754
Fragments arranged by size:
754 356 33
BanI G'GyrC_C
Cuts at: 0 297 1143
Size: 297 846
BbsI GAAGACnn'nnnn_
Cuts at: 0 1063 1143
Size: 1063 80
BciVI GTATCCnnnnn_n'
Cuts at: 0 165 1143
Size: 165 978
BfrBI ATG'CAT
Cuts at: 0 754 1143
Size: 754 389
BglI GCCn_nnn'nGGC
Cuts at: 0 248 306 1143
Size: 248 58 837
Fragments arranged by size:
837 248 58
Bme1580I G_kGCm'C
Cuts at: 0 26 1085 1143
Size: 26 1059 58
Fragments arranged by size:
1059 58 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 246 1143
Size: 246 897
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1143
Size: 959 184
BsaAI yAC'GTr
Cuts at: 0 1088 1143
Size: 1088 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 1143
Size: 31 1112
BseYI C'CCAG_C
Cuts at: 0 134 1143
Size: 134 1009
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1143
Size: 678 465
BsiEI CG_ry'CG
Cuts at: 0 16 92 1143
Size: 16 76 1051
Fragments arranged by size:
1051 76 16
BsiHKAI G_wGCw'C
Cuts at: 0 788 1143
Size: 788 355
BsmI GAATG_Cn'
Cuts at: 0 850 1143
Size: 850 293
Bsp1286I G_dGCh'C
Cuts at: 0 26 788 1085 1143
Size: 26 762 297 58
Fragments arranged by size:
762 297 58 26
BspHI T'CATG_A
Cuts at: 0 1020 1143
Size: 1020 123
BsrFI r'CCGG_y
Cuts at: 0 87 1143
Size: 87 1056
BtgI C'CryG_G
Cuts at: 0 26 1127 1143
Size: 26 1101 16
Fragments arranged by size:
1101 26 16
DraI TTT'AAA
Cuts at: 0 446 1143
Size: 446 697
EaeI y'GGCC_r
Cuts at: 0 13 89 1143
Size: 13 76 1054
Fragments arranged by size:
1054 76 13
EagI C'GGCC_G
Cuts at: 0 13 89 1143
Size: 13 76 1054
Fragments arranged by size:
1054 76 13
EarI CTCTTCn'nnn_
Cuts at: 0 266 396 1143
Size: 266 130 747
Fragments arranged by size:
747 266 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1040 1143
Size: 421 619 103
Fragments arranged by size:
619 421 103
EcoO109I rG'GnC_Cy
Cuts at: 0 873 1143
Size: 873 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 425 1143
Size: 425 718
HaeII r_GCGC'y
Cuts at: 0 34 1143
Size: 34 1109
HincII GTy'rAC
Cuts at: 0 1100 1143
Size: 1100 43
HindIII A'AGCT_T
Cuts at: 0 201 1143
Size: 201 942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1143
Size: 937 206
MslI CAynn'nnrTG
Cuts at: 0 843 1019 1143
Size: 843 176 124
Fragments arranged by size:
843 176 124
MspA1I CmG'CkG
Cuts at: 0 28 1143
Size: 28 1115
NaeI GCC'GGC
Cuts at: 0 89 1143
Size: 89 1054
NcoI C'CATG_G
Cuts at: 0 1127 1143
Size: 1127 16
NgoMIV G'CCGG_C
Cuts at: 0 87 1143
Size: 87 1056
NsiI A_TGCA'T
Cuts at: 0 756 1143
Size: 756 387
NspI r_CATG'y
Cuts at: 0 758 1143
Size: 758 385
PfoI T'CCnGG_A
Cuts at: 0 495 1000 1143
Size: 495 505 143
Fragments arranged by size:
505 495 143
PmlI CAC'GTG
Cuts at: 0 1088 1143
Size: 1088 55
PpuMI rG'GwC_Cy
Cuts at: 0 873 1143
Size: 873 270
SacII CC_GC'GG
Cuts at: 0 29 1143
Size: 29 1114
SexAI A'CCwGG_T
Cuts at: 0 528 1143
Size: 528 615
SfcI C'TryA_G
Cuts at: 0 917 1143
Size: 917 226
SmlI C'TyrA_G
Cuts at: 0 244 974 1143
Size: 244 730 169
Fragments arranged by size:
730 244 169
StyI C'CwwG_G
Cuts at: 0 869 1127 1143
Size: 869 258 16
Fragments arranged by size:
869 258 16
TatI w'GTAC_w
Cuts at: 0 799 988 1143
Size: 799 189 155
Fragments arranged by size:
799 189 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 1143
Size: 367 32 744
Fragments arranged by size:
744 367 32
XmnI GAAnn'nnTTC
Cuts at: 0 1053 1143
Size: 1053 90
Enzymes that do cut:
AcuI AflII AflIII ApoI AvaI BaeI BanI BbsI
BciVI BfrBI BglI Bme1580I BplI BpuEI BsaAI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrFI
BtgI DraI EaeI EagI EarI Eco57MI EcoO109I FalI
HaeII HincII HindIII MmeI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PfoI PmlI PpuMI SacII SexAI
SfcI SmlI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BamHI BanII BbeI BbvCI BcgI BclI
BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI Hin4I HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI