(Linear) (Six-base) MAPSORT of: KIEE1469.seq  Check: 1350  from: 1  to: 279



KIEE1469.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     279 
   Size:        192      87
 
AlwNI CAG_nnn'CTG

Cuts at:      0     104     279 
   Size:        104     175
 
AvaI C'yCGr_G

Cuts at:      0     180     279 
   Size:        180      99
 
BanI G'GyrC_C

Cuts at:      0      82     279 
   Size:         82     197
 
BbeI G_GCGC'C

Cuts at:      0      86     279 
   Size:         86     193
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     101     279 
   Size:        101     178
 
BglI GCCn_nnn'nGGC

Cuts at:      0      80     279 
   Size:         80     199
 
BglII A'GATC_T

Cuts at:      0     221     279 
   Size:        221      58
 
BlpI GC'TnA_GC

Cuts at:      0     271     279 
   Size:        271       8
 
Bme1580I G_kGCm'C

Cuts at:      0     245     279 
   Size:        245      34
 
BsaHI Gr'CG_yC

Cuts at:      0      83     279 
   Size:         83     196
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     279 
   Size:          2     277
 
Bsp1286I G_dGCh'C

Cuts at:      0     245     279 
   Size:        245      34
 
BstYI r'GATC_y

Cuts at:      0     221     279 
   Size:        221      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     279 
   Size:        192      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     180     279 
   Size:        180      99
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73     279 
   Size:         73     206
 
HaeII r_GCGC'y

Cuts at:      0      55      86     279 
   Size:         55      31     193

  Fragments arranged by size: 

                 193      55      31
 
KasI G'GCGC_C

Cuts at:      0      82     279 
   Size:         82     197
 
NarI GG'CG_CC

Cuts at:      0      83     279 
   Size:         83     196
 
PasI CC'CwG_GG

Cuts at:      0     245     279 
   Size:        245      34
 
SfcI C'TryA_G

Cuts at:      0      10     279 
   Size:         10     269
 
SfoI GGC'GCC

Cuts at:      0      84     279 
   Size:         84     195
 
StuI AGG'CCT

Cuts at:      0     186     279 
   Size:        186      93
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     237     279 
   Size:        237      42
 
Tth111I GACn'n_nGTC

Cuts at:      0     100     279 
   Size:        100     179
 
XmnI GAAnn'nnTTC

Cuts at:      0     154     279 
   Size:        154     125
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BanI     BbeI     BbsI     BglI    BglII
     BlpI Bme1580I    BsaHI     BsgI Bsp1286I    BstYI  Eco57MI    EcoNI
 EcoO109I    HaeII     KasI     NarI     PasI     SfcI     SfoI     StuI
    TaqII  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI     TatI     TstI     XbaI     XcmI     XhoI     XmaI
     ZraI