Restriction map for
pFN21AE1468
(Linear) (Six-base) MAPSORT of: KIEE1468.seq Check: 8533 from: 1 to: 2841
KIEE1468.orf
With 182 enzymes: SgfI *
July 3, 2012 16:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2624 2841
Size: 2624 217
AatII G_ACGT'C
Cuts at: 0 1547 2841
Size: 1547 1294
AccI GT'mk_AC
Cuts at: 0 116 869 920 2841
Size: 116 753 51 1921
Fragments arranged by size:
1921 753 116 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 2002 2679 2841
Size: 619 1383 677 162
Fragments arranged by size:
1383 677 619 162
AflIII A'CryG_T
Cuts at: 0 713 2841
Size: 713 2128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2779 2841
Size: 2779 62
AlwNI CAG_nnn'CTG
Cuts at: 0 1497 1715 1748 2254 2307 2316 2841
Size: 1497 218 33 506 53 9 525
Fragments arranged by size:
1497 525 506 218 53 33 9
ApaI G_GGCC'C
Cuts at: 0 1476 2091 2099 2841
Size: 1476 615 8 742
Fragments arranged by size:
1476 742 615 8
ApaLI G'TGCA_C
Cuts at: 0 1240 2769 2841
Size: 1240 1529 72
Fragments arranged by size:
1529 1240 72
ApoI r'AATT_y
Cuts at: 0 2378 2841
Size: 2378 463
AvaI C'yCGr_G
Cuts at: 0 1100 2540 2751 2841
Size: 1100 1440 211 90
Fragments arranged by size:
1440 1100 211 90
BanI G'GyrC_C
Cuts at: 0 28 556 976 2742 2841
Size: 28 528 420 1766 99
Fragments arranged by size:
1766 528 420 99 28
BanII G_rGCy'C
Cuts at: 0 112 145 451 959 1039 1093 1109 1476
Size: 112 33 306 508 80 54 16 367
Cuts at: 1476 1913 1990 2091 2099 2130 2162 2476 2841
Size: 437 77 101 8 31 32 314 365
Fragments arranged by size:
508 437 367 365 314 306 112 101
80 77 54 33 32 31 16 8
BbeI G_GCGC'C
Cuts at: 0 560 2841
Size: 560 2281
BbsI GAAGACnn'nnnn_
Cuts at: 0 88 1612 2841
Size: 88 1524 1229
Fragments arranged by size:
1524 1229 88
BbvCI CC'TCA_GC
Cuts at: 0 1900 2108 2392 2841
Size: 1900 208 284 449
Fragments arranged by size:
1900 449 284 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 285 319 2707 2741 2841
Size: 285 34 2388 34 100
Fragments arranged by size:
2388 285 100 34 34
BclI T'GATC_A
Cuts at: 0 342 686 2841
Size: 342 344 2155
Fragments arranged by size:
2155 344 342
BlpI GC'TnA_GC
Cuts at: 0 39 2841
Size: 39 2802
Bme1580I G_kGCm'C
Cuts at: 0 33 196 981 1244 1476 1962 2091 2099
Size: 33 163 785 263 232 486 129 8
Cuts at: 2099 2773 2841
Size: 674 68
Fragments arranged by size:
785 674 486 263 232 163 129 68
33 8
BmgBI CAC'GTC
Cuts at: 0 180 2841
Size: 180 2661
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 523 1203 2841
Size: 103 420 680 1638
Fragments arranged by size:
1638 680 420 103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1825 2841
Size: 1825 1016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 859 973 1077 1519 1758 2122 2263
Size: 799 60 114 104 442 239 364 141
Cuts at: 2263 2383 2502 2511 2820 2826 2841
Size: 120 119 9 309 6 15
Fragments arranged by size:
799 442 364 309 239 141 120 119
114 104 60 15 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 506 901 1038 1900 2108 2132 2392 2470
Size: 506 395 137 862 208 24 260 78
Cuts at: 2470 2475 2841
Size: 5 366
Fragments arranged by size:
862 506 395 366 260 208 137 78
24 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1141 1801 2841
Size: 145 996 660 1040
Fragments arranged by size:
1040 996 660 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 1672 2841
Size: 1672 1169
BsaAI yAC'GTr
Cuts at: 0 714 2841
Size: 714 2127
BsaHI Gr'CG_yC
Cuts at: 0 85 557 1544 1721 2841
Size: 85 472 987 177 1120
Fragments arranged by size:
1120 987 472 177 85
BsaWI w'CCGG_w
Cuts at: 0 1692 2841
Size: 1692 1149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 1934 1964 2392 2422 2841
Size: 529 30 1375 30 428 30 419
Fragments arranged by size:
1375 529 428 419 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1330 2125 2135 2171 2218 2380 2841
Size: 1330 795 10 36 47 162 461
Fragments arranged by size:
1330 795 461 162 47 36 10
BseYI C'CCAG_C
Cuts at: 0 307 958 1108 1704 1938 2063 2266 2348
Size: 307 651 150 596 234 125 203 82
Cuts at: 2348 2682 2841
Size: 334 159
Fragments arranged by size:
651 596 334 307 234 203 159 150
125 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 553 709 1352 1744 2602 2841
Size: 504 49 156 643 392 858 239
Fragments arranged by size:
858 643 504 392 239 156 49
BsiEI CG_ry'CG
Cuts at: 0 655 2841
Size: 655 2186
BsiHKAI G_wGCw'C
Cuts at: 0 112 451 1039 1244 1565 2476 2773 2841
Size: 112 339 588 205 321 911 297 68
Fragments arranged by size:
911 588 339 321 297 205 112 68
Bsp1286I G_dGCh'C
Cuts at: 0 33 112 145 196 451 959 981 1039
Size: 33 79 33 51 255 508 22 58
Cuts at: 1039 1093 1109 1244 1476 1565 1913 1962 1990
Size: 54 16 135 232 89 348 49 28
Cuts at: 1990 2091 2099 2130 2162 2476 2773 2841
Size: 101 8 31 32 314 297 68
Fragments arranged by size:
508 348 314 297 255 232 135 101
89 79 68 58 54 51 49 33
33 32 31 28 22 16 8
BspEI T'CCGG_A
Cuts at: 0 1692 2841
Size: 1692 1149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2624 2771 2841
Size: 2624 147 70
Fragments arranged by size:
2624 147 70
BsrBI CCG'CTC
Cuts at: 0 1809 2280 2841
Size: 1809 471 561
Fragments arranged by size:
1809 561 471
BsrDI GCAATG_nn'
Cuts at: 0 706 2841
Size: 706 2135
BsrFI r'CCGG_y
Cuts at: 0 654 2841
Size: 654 2187
BssSI C'ACGA_G
Cuts at: 0 971 2385 2841
Size: 971 1414 456
Fragments arranged by size:
1414 971 456
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1020 1715 2307 2841
Size: 1020 695 592 534
Fragments arranged by size:
1020 695 592 534
BstEII G'GTnAC_C
Cuts at: 0 2703 2841
Size: 2703 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 2841
Size: 360 2481
BstYI r'GATC_y
Cuts at: 0 167 347 1096 1850 2678 2841
Size: 167 180 749 754 828 163
Fragments arranged by size:
828 754 749 180 167 163
BtgI C'CryG_G
Cuts at: 0 131 2841
Size: 131 2710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 288 1589 2640 2841
Size: 225 63 1301 1051 201
Fragments arranged by size:
1301 1051 225 201 63
BtsI GCAGTG_nn'
Cuts at: 0 11 50 2773 2841
Size: 11 39 2723 68
Fragments arranged by size:
2723 68 39 11
DraIII CAC_nnn'GTG
Cuts at: 0 1923 2841
Size: 1923 918
EaeI y'GGCC_r
Cuts at: 0 482 652 1067 1605 2101 2820 2841
Size: 482 170 415 538 496 719 21
Fragments arranged by size:
719 538 496 482 415 170 21
EagI C'GGCC_G
Cuts at: 0 652 2841
Size: 652 2189
EarI CTCTTCn'nnn_
Cuts at: 0 133 439 1978 2841
Size: 133 306 1539 863
Fragments arranged by size:
1539 863 306 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1201 1826 2841
Size: 1201 625 1015
Fragments arranged by size:
1201 1015 625
EcoICRI GAG'CTC
Cuts at: 0 110 449 1037 2474 2841
Size: 110 339 588 1437 367
Fragments arranged by size:
1437 588 367 339 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 799 859 973 1077 1519 1758 2002
Size: 619 180 60 114 104 442 239 244
Cuts at: 2002 2122 2263 2383 2502 2511 2679 2820 2826
Size: 120 141 120 119 9 168 141 6
Cuts at: 2826 2841
Size: 15
Fragments arranged by size:
619 442 244 239 180 168 141 141
120 120 119 114 104 60 15 9
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1489 2841
Size: 1489 1352
EcoO109I rG'GnC_Cy
Cuts at: 0 500 645 745 1384 1473 1494 2087 2088
Size: 500 145 100 639 89 21 593 1
Cuts at: 2088 2095 2096 2841
Size: 7 1 745
Fragments arranged by size:
745 639 593 500 145 100 89 21
7 1 1
HaeII r_GCGC'y
Cuts at: 0 560 2841
Size: 560 2281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 327 359 529 561 659 691 1172 1204
Size: 327 32 170 32 98 32 481 32
Cuts at: 1204 2392 2424 2841
Size: 1188 32 417
Fragments arranged by size:
1188 481 417 327 170 98 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1395 2841
Size: 1395 1446
HindIII A'AGCT_T
Cuts at: 0 121 2841
Size: 121 2720
KasI G'GCGC_C
Cuts at: 0 556 2841
Size: 556 2285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 2396 2841
Size: 379 2017 445
Fragments arranged by size:
2017 445 379
MscI TGG'CCA
Cuts at: 0 484 1069 1607 2822 2841
Size: 484 585 538 1215 19
Fragments arranged by size:
1215 585 538 484 19
MslI CAynn'nnrTG
Cuts at: 0 529 1402 2740 2841
Size: 529 873 1338 101
Fragments arranged by size:
1338 873 529 101
MspA1I CmG'CkG
Cuts at: 0 226 962 1437 1745 1933 2107 2316 2522
Size: 226 736 475 308 188 174 209 206
Cuts at: 2522 2686 2826 2841
Size: 164 140 15
Fragments arranged by size:
736 475 308 226 209 206 188 174
164 140 15
NaeI GCC'GGC
Cuts at: 0 656 2841
Size: 656 2185
NarI GG'CG_CC
Cuts at: 0 557 2841
Size: 557 2284
NdeI CA'TA_TG
Cuts at: 0 564 2841
Size: 564 2277
NgoMIV G'CCGG_C
Cuts at: 0 654 2841
Size: 654 2187
NspI r_CATG'y
Cuts at: 0 1018 1331 1767 2841
Size: 1018 313 436 1074
Fragments arranged by size:
1074 1018 436 313
PasI CC'CwG_GG
Cuts at: 0 1428 2092 2841
Size: 1428 664 749
Fragments arranged by size:
1428 749 664
PflMI CCAn_nnn'nTGG
Cuts at: 0 613 1076 1497 2841
Size: 613 463 421 1344
Fragments arranged by size:
1344 613 463 421
PfoI T'CCnGG_A
Cuts at: 0 2294 2488 2624 2841
Size: 2294 194 136 217
Fragments arranged by size:
2294 217 194 136
PmlI CAC'GTG
Cuts at: 0 714 2841
Size: 714 2127
PpuMI rG'GwC_Cy
Cuts at: 0 500 2841
Size: 500 2341
PspOMI G'GGCC_C
Cuts at: 0 1472 2087 2095 2841
Size: 1472 615 8 746
Fragments arranged by size:
1472 746 615 8
PstI C_TGCA'G
Cuts at: 0 1932 2312 2691 2768 2841
Size: 1932 380 379 77 73
Fragments arranged by size:
1932 380 379 77 73
PvuII CAG'CTG
Cuts at: 0 226 962 1437 1745 2107 2316 2522 2686
Size: 226 736 475 308 362 209 206 164
Cuts at: 2686 2826 2841
Size: 140 15
Fragments arranged by size:
736 475 362 308 226 209 206 164
140 15
SacI G_AGCT'C
Cuts at: 0 112 451 1039 2476 2841
Size: 112 339 588 1437 365
Fragments arranged by size:
1437 588 365 339 112
SapI GCTCTTCn'nnn_
Cuts at: 0 133 439 1978 2841
Size: 133 306 1539 863
Fragments arranged by size:
1539 863 306 133
SfcI C'TryA_G
Cuts at: 0 371 777 1928 1963 2308 2687 2764 2841
Size: 371 406 1151 35 345 379 77 77
Fragments arranged by size:
1151 406 379 371 345 77 77 35
SfoI GGC'GCC
Cuts at: 0 558 2841
Size: 558 2283
SmaI CCC'GGG
Cuts at: 0 1102 2542 2841
Size: 1102 1440 299
Fragments arranged by size:
1440 1102 299
SmlI C'TyrA_G
Cuts at: 0 124 1156 1816 2841
Size: 124 1032 660 1025
Fragments arranged by size:
1032 1025 660 124
SphI G_CATG'C
Cuts at: 0 1018 2841
Size: 1018 1823
StuI AGG'CCT
Cuts at: 0 611 2446 2841
Size: 611 1835 395
Fragments arranged by size:
1835 611 395
StyI C'CwwG_G
Cuts at: 0 74 188 200 479 612 1387 1724 1990
Size: 74 114 12 279 133 775 337 266
Cuts at: 1990 2083 2841
Size: 93 758
Fragments arranged by size:
775 758 337 279 266 133 114 93
74 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1362 2841
Size: 1362 1479
TatI w'GTAC_w
Cuts at: 0 320 1478 2841
Size: 320 1158 1363
Fragments arranged by size:
1363 1158 320
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1934 1966 2841
Size: 1934 32 875
Fragments arranged by size:
1934 875 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2526 2841
Size: 2526 315
XmaI C'CCGG_G
Cuts at: 0 1100 2540 2841
Size: 1100 1440 301
Fragments arranged by size:
1440 1100 301
XmnI GAAnn'nnTTC
Cuts at: 0 1304 2841
Size: 1304 1537
ZraI GAC'GTC
Cuts at: 0 1545 2841
Size: 1545 1296
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BclI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SapI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BfrBI
BglI BglII BmtI BsaBI BsiWI BsmI BsmBI BspHI
BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KpnI MfeI MluI NcoI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI