Restriction map for
pFN21AE1467
(Linear) (Six-base) MAPSORT of: KIEE1467.seq Check: 9689 from: 1 to: 1710
KIEE1467.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 1321 1637 1710
Size: 509 812 316 73
Fragments arranged by size:
812 509 316 73
AleI CACnn'nnGTG
Cuts at: 0 895 1710
Size: 895 815
ApaI G_GGCC'C
Cuts at: 0 165 1169 1710
Size: 165 1004 541
Fragments arranged by size:
1004 541 165
ApaLI G'TGCA_C
Cuts at: 0 1477 1710
Size: 1477 233
ApoI r'AATT_y
Cuts at: 0 180 1555 1710
Size: 180 1375 155
Fragments arranged by size:
1375 180 155
AseI AT'TA_AT
Cuts at: 0 562 1710
Size: 562 1148
AvaI C'yCGr_G
Cuts at: 0 386 1673 1710
Size: 386 1287 37
Fragments arranged by size:
1287 386 37
BanI G'GyrC_C
Cuts at: 0 1010 1710
Size: 1010 700
BanII G_rGCy'C
Cuts at: 0 29 165 1169 1710
Size: 29 136 1004 541
Fragments arranged by size:
1004 541 136 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 60 279 1049 1710
Size: 60 219 770 661
Fragments arranged by size:
770 661 219 60
BbvCI CC'TCA_GC
Cuts at: 0 1420 1710
Size: 1420 290
BciVI GTATCCnnnnn_n'
Cuts at: 0 1066 1710
Size: 1066 644
BclI T'GATC_A
Cuts at: 0 1502 1710
Size: 1502 208
BglII A'GATC_T
Cuts at: 0 1313 1365 1710
Size: 1313 52 345
Fragments arranged by size:
1313 345 52
Bme1580I G_kGCm'C
Cuts at: 0 165 988 1169 1481 1710
Size: 165 823 181 312 229
Fragments arranged by size:
823 312 229 181 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 1554 1710
Size: 18 1536 156
Fragments arranged by size:
1536 156 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 45 1621 1710
Size: 45 1576 89
Fragments arranged by size:
1576 89 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1024 1402 1710
Size: 554 470 378 308
Fragments arranged by size:
554 470 378 308
Bpu10I CC'TnA_GC
Cuts at: 0 1420 1710
Size: 1420 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1710
Size: 1243 467
BsaAI yAC'GTr
Cuts at: 0 685 1710
Size: 685 1025
BsaWI w'CCGG_w
Cuts at: 0 590 1710
Size: 590 1120
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1504 1710
Size: 1504 206
BseYI C'CCAG_C
Cuts at: 0 54 1445 1710
Size: 54 1391 265
Fragments arranged by size:
1391 265 54
BsiHKAI G_wGCw'C
Cuts at: 0 1481 1710
Size: 1481 229
BsmI GAATG_Cn'
Cuts at: 0 387 772 1710
Size: 387 385 938
Fragments arranged by size:
938 387 385
Bsp1286I G_dGCh'C
Cuts at: 0 29 165 988 1169 1481 1710
Size: 29 136 823 181 312 229
Fragments arranged by size:
823 312 229 181 136 29
BspEI T'CCGG_A
Cuts at: 0 590 1710
Size: 590 1120
BsrBI CCG'CTC
Cuts at: 0 1225 1710
Size: 1225 485
BsrDI GCAATG_nn'
Cuts at: 0 347 1710
Size: 347 1363
BsrFI r'CCGG_y
Cuts at: 0 1411 1710
Size: 1411 299
BstBI TT'CG_AA
Cuts at: 0 270 1710
Size: 270 1440
BstYI r'GATC_y
Cuts at: 0 1313 1365 1710
Size: 1313 52 345
Fragments arranged by size:
1313 345 52
Bsu36I CC'TnA_GG
Cuts at: 0 235 1037 1710
Size: 235 802 673
Fragments arranged by size:
802 673 235
BtgI C'CryG_G
Cuts at: 0 663 1710
Size: 663 1047
BtsI GCAGTG_nn'
Cuts at: 0 1175 1710
Size: 1175 535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1086 1117 1121 1152 1710
Size: 1086 31 4 31 558
Fragments arranged by size:
1086 558 31 31 4
DraIII CAC_nnn'GTG
Cuts at: 0 551 1710
Size: 551 1159
EaeI y'GGCC_r
Cuts at: 0 155 1710
Size: 155 1555
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1020 1710
Size: 1020 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 554 1024 1321 1402 1637 1710
Size: 509 45 470 297 81 235 73
Fragments arranged by size:
509 470 297 235 81 73 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 234 491 1710
Size: 234 257 1219
Fragments arranged by size:
1219 257 234
EcoO109I rG'GnC_Cy
Cuts at: 0 161 162 823 1166 1710
Size: 161 1 661 343 544
Fragments arranged by size:
661 544 343 161 1
EcoRI G'AATT_C
Cuts at: 0 1555 1710
Size: 1555 155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1335 1710
Size: 1335 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 527 559 807 839 1710
Size: 527 32 248 32 871
Fragments arranged by size:
871 527 248 32 32
HincII GTy'rAC
Cuts at: 0 1576 1710
Size: 1576 134
MfeI C'AATT_G
Cuts at: 0 1565 1710
Size: 1565 145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1679 1710
Size: 1679 31
MscI TGG'CCA
Cuts at: 0 157 1710
Size: 157 1553
MslI CAynn'nnrTG
Cuts at: 0 895 1484 1710
Size: 895 589 226
Fragments arranged by size:
895 589 226
NaeI GCC'GGC
Cuts at: 0 1413 1710
Size: 1413 297
NcoI C'CATG_G
Cuts at: 0 663 1710
Size: 663 1047
NgoMIV G'CCGG_C
Cuts at: 0 1411 1710
Size: 1411 299
NspI r_CATG'y
Cuts at: 0 195 1348 1710
Size: 195 1153 362
Fragments arranged by size:
1153 362 195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1710
Size: 1078 32 600
Fragments arranged by size:
1078 600 32
PpuMI rG'GwC_Cy
Cuts at: 0 823 1710
Size: 823 887
PspOMI G'GGCC_C
Cuts at: 0 161 1165 1710
Size: 161 1004 545
Fragments arranged by size:
1004 545 161
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 665 697 1710
Size: 665 32 1013
Fragments arranged by size:
1013 665 32
PstI C_TGCA'G
Cuts at: 0 171 1710
Size: 171 1539
SanDI GG'GwC_CC
Cuts at: 0 823 1710
Size: 823 887
SfcI C'TryA_G
Cuts at: 0 167 928 1710
Size: 167 761 782
Fragments arranged by size:
782 761 167
SmlI C'TyrA_G
Cuts at: 0 1258 1710
Size: 1258 452
SphI G_CATG'C
Cuts at: 0 195 1710
Size: 195 1515
SspI AAT'ATT
Cuts at: 0 1279 1710
Size: 1279 431
StuI AGG'CCT
Cuts at: 0 497 1511 1710
Size: 497 1014 199
Fragments arranged by size:
1014 497 199
StyI C'CwwG_G
Cuts at: 0 663 893 1622 1710
Size: 663 230 729 88
Fragments arranged by size:
729 663 230 88
XmnI GAAnn'nnTTC
Cuts at: 0 67 946 1710
Size: 67 879 764
Fragments arranged by size:
879 764 67
Enzymes that do cut:
AcuI AleI ApaI ApaLI ApoI AseI AvaI BanI
BanII BbsI BbvCI BciVI BclI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaWI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BstBI
BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MfeI
MmeI MscI MslI NaeI NcoI NgoMIV NspI PpiI
PpuMI PspOMI PsrI PstI SanDI SfcI SmlI SphI
SspI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI AscI AsiSI AvrII
BaeI BamHI BbeI BcgI BfrBI BglI BlpI BmgBI
BmtI BsaI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI
BsmBI BspHI BspMI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I BtgZI ClaI DraI DrdI EagI EarI
EcoICRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MspA1I NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspXI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI