Restriction map for
pFN21AE1465
(Linear) (Six-base) MAPSORT of: KIEE1465.seq Check: 1470 from: 1 to: 1128
KIEE1465.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1026 1128
Size: 1026 102
AatII G_ACGT'C
Cuts at: 0 1016 1128
Size: 1016 112
AccI GT'mk_AC
Cuts at: 0 767 1128
Size: 767 361
AleI CACnn'nnGTG
Cuts at: 0 1006 1128
Size: 1006 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1093 1128
Size: 1093 35
AlwNI CAG_nnn'CTG
Cuts at: 0 998 1041 1128
Size: 998 43 87
Fragments arranged by size:
998 87 43
BanI G'GyrC_C
Cuts at: 0 464 711 798 1128
Size: 464 247 87 330
Fragments arranged by size:
464 330 247 87
BanII G_rGCy'C
Cuts at: 0 35 542 1128
Size: 35 507 586
Fragments arranged by size:
586 507 35
BbeI G_GCGC'C
Cuts at: 0 802 1128
Size: 802 326
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 1128
Size: 441 687
BbvCI CC'TCA_GC
Cuts at: 0 460 729 1128
Size: 460 269 399
Fragments arranged by size:
460 399 269
BglI GCCn_nnn'nGGC
Cuts at: 0 720 1128
Size: 720 408
BglII A'GATC_T
Cuts at: 0 560 1128
Size: 560 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 922 1128
Size: 48 874 206
Fragments arranged by size:
874 206 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 490 607 616 964 1128
Size: 194 296 117 9 348 164
Fragments arranged by size:
348 296 194 164 117 9
Bpu10I CC'TnA_GC
Cuts at: 0 460 729 1128
Size: 460 269 399
Fragments arranged by size:
460 399 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 757 907 1128
Size: 274 483 150 221
Fragments arranged by size:
483 274 221 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 1128
Size: 136 992
BsaHI Gr'CG_yC
Cuts at: 0 368 799 1013 1128
Size: 368 431 214 115
Fragments arranged by size:
431 368 214 115
BsaWI w'CCGG_w
Cuts at: 0 665 1128
Size: 665 463
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 1128
Size: 463 30 635
Fragments arranged by size:
635 463 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1128
Size: 984 144
BsiHKAI G_wGCw'C
Cuts at: 0 542 758 1128
Size: 542 216 370
Fragments arranged by size:
542 370 216
Bsp1286I G_dGCh'C
Cuts at: 0 35 542 758 1128
Size: 35 507 216 370
Fragments arranged by size:
507 370 216 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 1026 1128
Size: 305 721 102
Fragments arranged by size:
721 305 102
BsrGI T'GTAC_A
Cuts at: 0 746 1128
Size: 746 382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 998 1041 1128
Size: 998 43 87
Fragments arranged by size:
998 87 43
BstYI r'GATC_y
Cuts at: 0 213 560 632 920 1128
Size: 213 347 72 288 208
Fragments arranged by size:
347 288 213 208 72
BtgI C'CryG_G
Cuts at: 0 260 408 1004 1128
Size: 260 148 596 124
Fragments arranged by size:
596 260 148 124
BtsI GCAGTG_nn'
Cuts at: 0 10 1128
Size: 10 1118
EaeI y'GGCC_r
Cuts at: 0 263 1128
Size: 263 865
EarI CTCTTCn'nnn_
Cuts at: 0 40 1128
Size: 40 1088
EcoICRI GAG'CTC
Cuts at: 0 540 1128
Size: 540 588
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 490 607 616 964 1128
Size: 194 296 117 9 348 164
Fragments arranged by size:
348 296 194 164 117 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 706 1128
Size: 46 660 422
Fragments arranged by size:
660 422 46
EcoO109I rG'GnC_Cy
Cuts at: 0 570 660 1128
Size: 570 90 468
Fragments arranged by size:
570 468 90
HaeII r_GCGC'y
Cuts at: 0 802 1076 1128
Size: 802 274 52
Fragments arranged by size:
802 274 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 1128
Size: 498 32 598
Fragments arranged by size:
598 498 32
HincII GTy'rAC
Cuts at: 0 933 1128
Size: 933 195
KasI G'GCGC_C
Cuts at: 0 798 1128
Size: 798 330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 884 1128
Size: 645 239 244
Fragments arranged by size:
645 244 239
MslI CAynn'nnrTG
Cuts at: 0 265 761 1006 1128
Size: 265 496 245 122
Fragments arranged by size:
496 265 245 122
MspA1I CmG'CkG
Cuts at: 0 744 912 1128
Size: 744 168 216
Fragments arranged by size:
744 216 168
NarI GG'CG_CC
Cuts at: 0 799 1128
Size: 799 329
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1128
Size: 642 486
PfoI T'CCnGG_A
Cuts at: 0 359 635 1128
Size: 359 276 493
Fragments arranged by size:
493 359 276
PpuMI rG'GwC_Cy
Cuts at: 0 570 1128
Size: 570 558
PsiI TTA'TAA
Cuts at: 0 695 1128
Size: 695 433
PstI C_TGCA'G
Cuts at: 0 917 1037 1128
Size: 917 120 91
Fragments arranged by size:
917 120 91
PvuII CAG'CTG
Cuts at: 0 744 912 1128
Size: 744 168 216
Fragments arranged by size:
744 216 168
SacI G_AGCT'C
Cuts at: 0 542 1128
Size: 542 586
SanDI GG'GwC_CC
Cuts at: 0 570 1128
Size: 570 558
SapI GCTCTTCn'nnn_
Cuts at: 0 40 1128
Size: 40 1088
SfcI C'TryA_G
Cuts at: 0 913 1033 1128
Size: 913 120 95
Fragments arranged by size:
913 120 95
SfoI GGC'GCC
Cuts at: 0 800 1128
Size: 800 328
SmlI C'TyrA_G
Cuts at: 0 289 736 886 1128
Size: 289 447 150 242
Fragments arranged by size:
447 289 242 150
StyI C'CwwG_G
Cuts at: 0 960 1128
Size: 960 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 1128
Size: 264 864
TatI w'GTAC_w
Cuts at: 0 269 329 746 1128
Size: 269 60 417 382
Fragments arranged by size:
417 382 269 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 239 271 1128
Size: 239 32 857
Fragments arranged by size:
857 239 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 582 849 1128
Size: 432 150 267 279
Fragments arranged by size:
432 279 267 150
XmnI GAAnn'nnTTC
Cuts at: 0 448 1128
Size: 448 680
ZraI GAC'GTC
Cuts at: 0 1014 1128
Size: 1014 114
Enzymes that do cut:
AarI AatII AccI AleI AlfI AlwNI BanI BanII
BbeI BbsI BbvCI BglI BglII BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I
BspMI BsrGI BstAPI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MmeI MslI MspA1I NarI PflMI PfoI PpuMI PsiI
PstI PvuII SacI SanDI SapI SfcI SfoI SmlI
StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI
Bme1580I BmgBI BmtI BplI BsaAI BsaBI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI