(Linear) (Six-base) MAPSORT of: KIEE1464.seq  Check: 5558  from: 1  to: 558



KIEE1464.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AccI GT'mk_AC

Cuts at:      0     458     558 
   Size:        458     100
 
AleI CACnn'nnGTG

Cuts at:      0     321     558 
   Size:        321     237
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     437     558 
   Size:        437     121
 
BanI G'GyrC_C

Cuts at:      0      17     558 
   Size:         17     541
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     179     558 
   Size:        179     379
 
BclI T'GATC_A

Cuts at:      0     339     558 
   Size:        339     219
 
Bme1580I G_kGCm'C

Cuts at:      0      22     558 
   Size:         22     536
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     558 
   Size:        120     438
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     118     148     451     481     558 
   Size:        118      30     303      30      77

  Fragments arranged by size: 

                 303     118      77      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     558 
   Size:        281     277
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     558 
   Size:         22     536
 
BspHI T'CATG_A

Cuts at:      0     501     558 
   Size:        501      57
 
BsrGI T'GTAC_A

Cuts at:      0     125     558 
   Size:        125     433
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     430     558 
   Size:        430     128
 
DraIII CAC_nnn'GTG

Cuts at:      0      99     558 
   Size:         99     459
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     558 
   Size:        120     438
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     468     558 
   Size:        468      90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     451     483     558 
   Size:        451      32      75

  Fragments arranged by size: 

                 451      75      32
 
MslI CAynn'nnrTG

Cuts at:      0     321     558 
   Size:        321     237
 
NspI r_CATG'y

Cuts at:      0     424     558 
   Size:        424     134
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      99     187     558 
   Size:         99      88     371

  Fragments arranged by size: 

                 371      99      88
 
SspI AAT'ATT

Cuts at:      0      87     558 
   Size:         87     471
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       5     558 
   Size:          5     553
 
TatI w'GTAC_w

Cuts at:      0     125     558 
   Size:        125     433
 
XmnI GAAnn'nnTTC

Cuts at:      0     497     558 
   Size:        497      61
 

 Enzymes that do cut:

     AccI     AleI     AlfI     BanI     BbsI     BclI Bme1580I     BpmI
    BsaXI     BsgI Bsp1286I    BspHI    BsrGI   BstAPI   DraIII  Eco57MI
     FalI    Hin4I     MslI     NspI    PflMI     SspI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI