(Linear) (Six-base) MAPSORT of: KIEE1462.seq  Check: 5669  from: 1  to: 708



KIEE1462.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     708 
   Size:        172     536
 
AhdI GACnn_n'nnGTC

Cuts at:      0     627     708 
   Size:        627      81
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       1      33     708 
   Size:          1      32     675

  Fragments arranged by size: 

                 675      32       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0     150     708 
   Size:        150     558
 
ApoI r'AATT_y

Cuts at:      0      68     708 
   Size:         68     640
 
AvaI C'yCGr_G

Cuts at:      0     263     708 
   Size:        263     445
 
BanI G'GyrC_C

Cuts at:      0       3     108     301     708 
   Size:          3     105     193     407

  Fragments arranged by size: 

                 407     193     105       3
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     240     708 
   Size:        240     468
 
BclI T'GATC_A

Cuts at:      0     647     708 
   Size:        647      61
 
BglII A'GATC_T

Cuts at:      0     312     708 
   Size:        312     396
 
BlpI GC'TnA_GC

Cuts at:      0     347     708 
   Size:        347     361
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     385     708 
   Size:        385     323
 
BsaWI w'CCGG_w

Cuts at:      0       6     708 
   Size:          6     702
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     368     394     403     708 
   Size:        368      26       9     305

  Fragments arranged by size: 

                 368     305      26       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     360     708 
   Size:        360     348
 
Bsp1286I G_dGCh'C

Cuts at:      0     360     708 
   Size:        360     348
 
BsrDI GCAATG_nn'

Cuts at:      0     600     708 
   Size:        600     108
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     402     567     708 
   Size:        402     165     141

  Fragments arranged by size: 

                 402     165     141
 
BstYI r'GATC_y

Cuts at:      0     312     708 
   Size:        312     396
 
EaeI y'GGCC_r

Cuts at:      0     697     708 
   Size:        697      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     335     708 
   Size:        335     373
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     708 
   Size:        172     536
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     172     708 
   Size:        172     536
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     259     613     645     708 
   Size:        227      32     354      32      63

  Fragments arranged by size: 

                 354     227      63      32      32
 
HincII GTy'rAC

Cuts at:      0     200     708 
   Size:        200     508
 
HindIII A'AGCT_T

Cuts at:      0     115     708 
   Size:        115     593
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     368     708 
   Size:        368     340
 
SmlI C'TyrA_G

Cuts at:      0     263     708 
   Size:        263     445
 
StuI AGG'CCT

Cuts at:      0     461     708 
   Size:        461     247
 
StyI C'CwwG_G

Cuts at:      0     367     708 
   Size:        367     341
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     245     375     708 
   Size:        245     130     333

  Fragments arranged by size: 

                 333     245     130
 
XhoI C'TCGA_G

Cuts at:      0     263     708 
   Size:        263     445
 
XmnI GAAnn'nnTTC

Cuts at:      0     587     708 
   Size:        587     121
 

 Enzymes that do cut:

     AcuI     AhdI     AloI    AlwNI     ApoI     AvaI     BanI     BbsI
     BclI    BglII     BlpI     BsaI    BsaWI    BseRI  BsiHKAI Bsp1286I
    BsrDI    BstXI    BstYI     EaeI     EarI  Eco57MI    EcoNI    Hin4I
   HincII  HindIII    PflMI     SmlI     StuI     StyI    TaqII     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     ZraI