Restriction map for
pFN21AE1461
(Linear) (Six-base) MAPSORT of: KIEE1461.seq Check: 1735 from: 1 to: 1476
KIEE1461.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 152 1476
Size: 152 1324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1476
Size: 286 1190
AlwNI CAG_nnn'CTG
Cuts at: 0 309 633 1014 1476
Size: 309 324 381 462
Fragments arranged by size:
462 381 324 309
ApaI G_GGCC'C
Cuts at: 0 466 736 1226 1298 1476
Size: 466 270 490 72 178
Fragments arranged by size:
490 466 270 178 72
ApaLI G'TGCA_C
Cuts at: 0 852 1476
Size: 852 624
AvaI C'yCGr_G
Cuts at: 0 275 392 668 1044 1127 1225 1308 1421
Size: 275 117 276 376 83 98 83 113
Cuts at: 1421 1476
Size: 55
Fragments arranged by size:
376 276 275 117 113 98 83 83
55
BanI G'GyrC_C
Cuts at: 0 509 648 1432 1476
Size: 509 139 784 44
Fragments arranged by size:
784 509 139 44
BanII G_rGCy'C
Cuts at: 0 103 399 466 736 910 1058 1226 1232
Size: 103 296 67 270 174 148 168 6
Cuts at: 1232 1298 1315 1416 1476
Size: 66 17 101 60
Fragments arranged by size:
296 270 174 168 148 103 101 67
66 60 17 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1188 1476
Size: 1188 288
BbvCI CC'TCA_GC
Cuts at: 0 281 1476
Size: 281 1195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 164 1476
Size: 130 34 1312
Fragments arranged by size:
1312 130 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1181 1236 1476
Size: 1181 55 240
Fragments arranged by size:
1181 240 55
BlpI GC'TnA_GC
Cuts at: 0 377 1476
Size: 377 1099
Bme1580I G_kGCm'C
Cuts at: 0 466 736 856 1226 1298 1476
Size: 466 270 120 370 72 178
Fragments arranged by size:
466 370 270 178 120 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 1476
Size: 117 1359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 476 918 1476
Size: 417 59 442 558
Fragments arranged by size:
558 442 417 59
Bpu10I CC'TnA_GC
Cuts at: 0 281 1476
Size: 281 1195
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 1274 1476
Size: 1093 181 202
Fragments arranged by size:
1093 202 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 415 445 641 671 1476
Size: 199 30 186 30 196 30 805
Fragments arranged by size:
805 199 196 186 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 928 1105 1476
Size: 928 177 371
Fragments arranged by size:
928 371 177
BseYI C'CCAG_C
Cuts at: 0 96 401 781 842 1007 1014 1027 1271
Size: 96 305 380 61 165 7 13 244
Cuts at: 1271 1476
Size: 205
Fragments arranged by size:
380 305 244 205 165 96 61 13
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 943 1476
Size: 124 819 533
Fragments arranged by size:
819 533 124
BsiEI CG_ry'CG
Cuts at: 0 1219 1476
Size: 1219 257
BsiHKAI G_wGCw'C
Cuts at: 0 856 1416 1476
Size: 856 560 60
Fragments arranged by size:
856 560 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1418 1476
Size: 1418 58
Bsp1286I G_dGCh'C
Cuts at: 0 103 399 466 736 856 910 1058 1226
Size: 103 296 67 270 120 54 148 168
Cuts at: 1226 1232 1298 1315 1416 1476
Size: 6 66 17 101 60
Fragments arranged by size:
296 270 168 148 120 103 101 67
66 60 54 17 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 980 1412 1475 1476
Size: 152 828 432 63 1
Fragments arranged by size:
828 432 152 63 1
BsrDI GCAATG_nn'
Cuts at: 0 545 1476
Size: 545 931
BsrFI r'CCGG_y
Cuts at: 0 585 1214 1476
Size: 585 629 262
Fragments arranged by size:
629 585 262
BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 1476
Size: 633 843
BstYI r'GATC_y
Cuts at: 0 306 1476
Size: 306 1170
Bsu36I CC'TnA_GG
Cuts at: 0 638 1289 1476
Size: 638 651 187
Fragments arranged by size:
651 638 187
BtgI C'CryG_G
Cuts at: 0 1146 1179 1388 1476
Size: 1146 33 209 88
Fragments arranged by size:
1146 209 88 33
BtsI GCAGTG_nn'
Cuts at: 0 322 618 979 1105 1476
Size: 322 296 361 126 371
Fragments arranged by size:
371 361 322 296 126
DraIII CAC_nnn'GTG
Cuts at: 0 759 1476
Size: 759 717
EaeI y'GGCC_r
Cuts at: 0 228 587 1216 1391 1476
Size: 228 359 629 175 85
Fragments arranged by size:
629 359 228 175 85
EagI C'GGCC_G
Cuts at: 0 1216 1476
Size: 1216 260
EarI CTCTTCn'nnn_
Cuts at: 0 217 335 521 1071 1476
Size: 217 118 186 550 405
Fragments arranged by size:
550 405 217 186 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1201 1476
Size: 1201 275
EcoICRI GAG'CTC
Cuts at: 0 1414 1476
Size: 1414 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 417 476 918 1476
Size: 286 131 59 442 558
Fragments arranged by size:
558 442 286 131 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 637 661 1476
Size: 301 336 24 815
Fragments arranged by size:
815 336 301 24
EcoO109I rG'GnC_Cy
Cuts at: 0 462 673 687 771 822 1222 1294 1295
Size: 462 211 14 84 51 400 72 1
Cuts at: 1295 1476
Size: 181
Fragments arranged by size:
462 400 211 181 84 72 51 14
1
HaeII r_GCGC'y
Cuts at: 0 192 1476
Size: 192 1284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 729 761 1027 1059 1476
Size: 547 32 150 32 266 32 417
Fragments arranged by size:
547 417 266 150 32 32 32
HincII GTy'rAC
Cuts at: 0 579 1476
Size: 579 897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1424 1476
Size: 230 1194 52
Fragments arranged by size:
1194 230 52
MscI TGG'CCA
Cuts at: 0 1393 1476
Size: 1393 83
MslI CAynn'nnrTG
Cuts at: 0 152 267 331 572 1255 1476
Size: 152 115 64 241 683 221
Fragments arranged by size:
683 241 221 152 115 64
MspA1I CmG'CkG
Cuts at: 0 17 167 376 1014 1181 1476
Size: 17 150 209 638 167 295
Fragments arranged by size:
638 295 209 167 150 17
NaeI GCC'GGC
Cuts at: 0 1216 1476
Size: 1216 260
NgoMIV G'CCGG_C
Cuts at: 0 1214 1476
Size: 1214 262
PflMI CCAn_nnn'nTGG
Cuts at: 0 1014 1476
Size: 1014 462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 1476
Size: 20 32 1424
Fragments arranged by size:
1424 32 20
PpuMI rG'GwC_Cy
Cuts at: 0 673 687 1476
Size: 673 14 789
Fragments arranged by size:
789 673 14
PspOMI G'GGCC_C
Cuts at: 0 462 732 1222 1294 1476
Size: 462 270 490 72 182
Fragments arranged by size:
490 462 270 182 72
PspXI vC'TCGA_Gb
Cuts at: 0 392 1044 1308 1476
Size: 392 652 264 168
Fragments arranged by size:
652 392 264 168
PstI C_TGCA'G
Cuts at: 0 16 629 1112 1409 1476
Size: 16 613 483 297 67
Fragments arranged by size:
613 483 297 67 16
PvuII CAG'CTG
Cuts at: 0 17 1014 1476
Size: 17 997 462
Fragments arranged by size:
997 462 17
SacI G_AGCT'C
Cuts at: 0 1416 1476
Size: 1416 60
SacII CC_GC'GG
Cuts at: 0 1182 1476
Size: 1182 294
SanDI GG'GwC_CC
Cuts at: 0 687 1476
Size: 687 789
SapI GCTCTTCn'nnn_
Cuts at: 0 335 1476
Size: 335 1141
SfcI C'TryA_G
Cuts at: 0 12 625 1108 1405 1476
Size: 12 613 483 297 71
Fragments arranged by size:
613 483 297 71 12
SmaI CCC'GGG
Cuts at: 0 277 670 1129 1476
Size: 277 393 459 347
Fragments arranged by size:
459 393 347 277
SmlI C'TyrA_G
Cuts at: 0 392 1044 1308 1476
Size: 392 652 264 168
Fragments arranged by size:
652 392 264 168
SspI AAT'ATT
Cuts at: 0 257 1476
Size: 257 1219
StyI C'CwwG_G
Cuts at: 0 1189 1234 1476
Size: 1189 45 242
Fragments arranged by size:
1189 242 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 423 550 1351 1476
Size: 423 127 801 125
Fragments arranged by size:
801 423 127 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 1476
Size: 641 32 803
Fragments arranged by size:
803 641 32
XhoI C'TCGA_G
Cuts at: 0 392 1044 1308 1476
Size: 392 652 264 168
Fragments arranged by size:
652 392 264 168
XmaI C'CCGG_G
Cuts at: 0 275 668 1127 1476
Size: 275 393 459 349
Fragments arranged by size:
459 393 349 275
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BbvCI BcgI BglI BlpI Bme1580I BplI BpmI
Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NgoMIV PflMI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SanDI SapI SfcI
SmaI SmlI SspI StyI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI
BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I
XbaI XcmI XmnI ZraI