(Linear) (Six-base) MAPSORT of: KIEE1460.seq  Check: 6186  from: 1  to: 999



KIEE1460.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     302     999 
   Size:         98     204     697

  Fragments arranged by size: 

                 697     204      98
 
AflII C'TTAA_G

Cuts at:      0     301     999 
   Size:        301     698
 
AlwNI CAG_nnn'CTG

Cuts at:      0     493     999 
   Size:        493     506
 
ApoI r'AATT_y

Cuts at:      0     601     999 
   Size:        601     398
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     452     999 
   Size:        452     547
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      81     999 
   Size:         81     918
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     997     999 
   Size:        997       2
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     831     999 
   Size:        831     168
 
BsaWI w'CCGG_w

Cuts at:      0     858     999 
   Size:        858     141
 
BseYI C'CCAG_C

Cuts at:      0     107     586     999 
   Size:        107     479     413

  Fragments arranged by size: 

                 479     413     107
 
BsiHKAI G_wGCw'C

Cuts at:      0     978     999 
   Size:        978      21
 
Bsp1286I G_dGCh'C

Cuts at:      0     978     999 
   Size:        978      21
 
BspHI T'CATG_A

Cuts at:      0     557     608     999 
   Size:        557      51     391

  Fragments arranged by size: 

                 557     391      51
 
BsrFI r'CCGG_y

Cuts at:      0     946     999 
   Size:        946      53
 
BstYI r'GATC_y

Cuts at:      0     297     999 
   Size:        297     702
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     110     145     999 
   Size:        110      35     854

  Fragments arranged by size: 

                 854     110      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     999 
   Size:        167     832
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     302     999 
   Size:         98     204     697

  Fragments arranged by size: 

                 697     204      98
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     874     999 
   Size:        874     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0     490     999 
   Size:        490     509
 
EcoRV GAT'ATC

Cuts at:      0     672     999 
   Size:        672     327
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       9      41     454     486     999 
   Size:          9      32     413      32     513

  Fragments arranged by size: 

                 513     413      32      32       9
 
HincII GTy'rAC

Cuts at:      0     660     999 
   Size:        660     339
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     810     999 
   Size:        810     189
 
NspI r_CATG'y

Cuts at:      0     851     999 
   Size:        851     148
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     726     758     999 
   Size:        726      32     241

  Fragments arranged by size: 

                 726     241      32
 
PstI C_TGCA'G

Cuts at:      0     875     999 
   Size:        875     124
 
SfcI C'TryA_G

Cuts at:      0     746     871     898     999 
   Size:        746     125      27     101

  Fragments arranged by size: 

                 746     125     101      27
 
SmlI C'TyrA_G

Cuts at:      0     301     810     999 
   Size:        301     509     189

  Fragments arranged by size: 

                 509     301     189
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     611     643     999 
   Size:        611      32     356

  Fragments arranged by size: 

                 611     356      32
 
XbaI T'CTAG_A

Cuts at:      0     207     435     919     999 
   Size:        207     228     484      80

  Fragments arranged by size: 

                 484     228     207      80
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     BbsI    BciVI     BplI    BpuEI
    BsaWI    BseYI  BsiHKAI Bsp1286I    BspHI    BsrFI    BstYI    CspCI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRV    Hin4I   HincII     MmeI
     NspI     PsrI     PstI     SfcI     SmlI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI