Restriction map for
pFN21AE1457
(Linear) (Six-base) MAPSORT of: KIEE1457.seq Check: 9208 from: 1 to: 3807
KIEE1457.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 533 620 1001 1320 1764 2475 3807
Size: 533 87 381 319 444 711 1332
Fragments arranged by size:
1332 711 533 444 381 319 87
AccI GT'mk_AC
Cuts at: 0 3611 3807
Size: 3611 196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 292 901 1357 2174 2988 3682 3807
Size: 133 159 609 456 817 814 694 125
Fragments arranged by size:
817 814 694 609 456 159 133 125
AflIII A'CryG_T
Cuts at: 0 2348 3490 3614 3807
Size: 2348 1142 124 193
Fragments arranged by size:
2348 1142 193 124
AhdI GACnn_n'nnGTC
Cuts at: 0 2340 3807
Size: 2340 1467
AleI CACnn'nnGTG
Cuts at: 0 13 3807
Size: 13 3794
AlwNI CAG_nnn'CTG
Cuts at: 0 171 606 1335 1437 2304 2418 2819 3331
Size: 171 435 729 102 867 114 401 512
Cuts at: 3331 3535 3807
Size: 204 272
Fragments arranged by size:
867 729 512 435 401 272 204 171
114 102
ApaI G_GGCC'C
Cuts at: 0 1259 1404 1986 3807
Size: 1259 145 582 1821
Fragments arranged by size:
1821 1259 582 145
ApaLI G'TGCA_C
Cuts at: 0 520 805 3807
Size: 520 285 3002
Fragments arranged by size:
3002 520 285
ApoI r'AATT_y
Cuts at: 0 276 897 3807
Size: 276 621 2910
Fragments arranged by size:
2910 621 276
AvaI C'yCGr_G
Cuts at: 0 145 257 715 1397 2467 2568 3807
Size: 145 112 458 682 1070 101 1239
Fragments arranged by size:
1239 1070 682 458 145 112 101
BanI G'GyrC_C
Cuts at: 0 711 1013 1210 2473 2823 3148 3807
Size: 711 302 197 1263 350 325 659
Fragments arranged by size:
1263 711 659 350 325 302 197
BanII G_rGCy'C
Cuts at: 0 1259 1404 1986 2096 2129 2569 3807
Size: 1259 145 582 110 33 440 1238
Fragments arranged by size:
1259 1238 582 440 145 110 33
BbeI G_GCGC'C
Cuts at: 0 3152 3807
Size: 3152 655
BbsI GAAGACnn'nnnn_
Cuts at: 0 1844 2162 2711 2796 2864 3359 3807
Size: 1844 318 549 85 68 495 448
Fragments arranged by size:
1844 549 495 448 318 85 68
BbvCI CC'TCA_GC
Cuts at: 0 43 400 472 757 855 1024 2906 3807
Size: 43 357 72 285 98 169 1882 901
Fragments arranged by size:
1882 901 357 285 169 98 72 43
BglI GCCn_nnn'nGGC
Cuts at: 0 253 2471 3346 3720 3807
Size: 253 2218 875 374 87
Fragments arranged by size:
2218 875 374 253 87
BglII A'GATC_T
Cuts at: 0 1631 3807
Size: 1631 2176
BlpI GC'TnA_GC
Cuts at: 0 871 3655 3807
Size: 871 2784 152
Fragments arranged by size:
2784 871 152
Bme1580I G_kGCm'C
Cuts at: 0 358 524 716 809 1018 1259 1404 1907
Size: 358 166 192 93 209 241 145 503
Cuts at: 1907 1986 2476 3450 3807
Size: 79 490 974 357
Fragments arranged by size:
974 503 490 358 357 241 209 192
166 145 93 79
BmgBI CAC'GTC
Cuts at: 0 681 1482 2349 3493 3807
Size: 681 801 867 1144 314
Fragments arranged by size:
1144 867 801 681 314
BmrI ACTGGGnnnn_n'
Cuts at: 0 1635 2125 2829 3807
Size: 1635 490 704 978
Fragments arranged by size:
1635 978 704 490
BmtI G_CTAG'C
Cuts at: 0 1207 3807
Size: 1207 2600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3561 3807
Size: 3561 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 508 676 1006 1459 2233 2755 3067
Size: 388 120 168 330 453 774 522 312
Cuts at: 3067 3213 3801 3807
Size: 146 588 6
Fragments arranged by size:
774 588 522 453 388 330 312 168
146 120 6
Bpu10I CC'TnA_GC
Cuts at: 0 43 400 472 757 855 1024 2906 3807
Size: 43 357 72 285 98 169 1882 901
Fragments arranged by size:
1882 901 357 285 169 98 72 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1576 1894 2386 3247 3807
Size: 351 1225 318 492 861 560
Fragments arranged by size:
1225 861 560 492 351 318
BsaI GGTCTCn'nnnn_
Cuts at: 0 2578 3807
Size: 2578 1229
BsaAI yAC'GTr
Cuts at: 0 810 1971 2148 3807
Size: 810 1161 177 1659
Fragments arranged by size:
1659 1161 810 177
BsaHI Gr'CG_yC
Cuts at: 0 2597 3149 3807
Size: 2597 552 658
Fragments arranged by size:
2597 658 552
BsaWI w'CCGG_w
Cuts at: 0 280 3500 3807
Size: 280 3220 307
Fragments arranged by size:
3220 307 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1600 1630 1777 1807 2224 2254 3211 3241
Size: 1600 30 147 30 417 30 957 30
Cuts at: 3241 3807
Size: 566
Fragments arranged by size:
1600 957 566 417 147 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 1396 1756 1777 2707 2782 2854 2857
Size: 163 1233 360 21 930 75 72 3
Cuts at: 2857 2881 2929 2944 3226 3427 3807
Size: 24 48 15 282 201 380
Fragments arranged by size:
1233 930 380 360 282 201 163 75
72 48 24 21 15 3
BseYI C'CCAG_C
Cuts at: 0 582 623 736 1405 2406 3160 3807
Size: 582 41 113 669 1001 754 647
Fragments arranged by size:
1001 754 669 647 582 113 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 2101 2416 3183 3807
Size: 592 1509 315 767 624
Fragments arranged by size:
1509 767 624 592 315
BsiEI CG_ry'CG
Cuts at: 0 2367 3715 3807
Size: 2367 1348 92
Fragments arranged by size:
2367 1348 92
BsiHKAI G_wGCw'C
Cuts at: 0 524 542 622 809 1592 2096 3508 3807
Size: 524 18 80 187 783 504 1412 299
Fragments arranged by size:
1412 783 524 504 299 187 80 18
BsmI GAATG_Cn'
Cuts at: 0 2291 3807
Size: 2291 1516
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3499 3807
Size: 3499 308
Bsp1286I G_dGCh'C
Cuts at: 0 358 524 542 622 716 809 1018 1259
Size: 358 166 18 80 94 93 209 241
Cuts at: 1259 1404 1592 1907 1986 2096 2129 2476 2569
Size: 145 188 315 79 110 33 347 93
Cuts at: 2569 3450 3508 3807
Size: 881 58 299
Fragments arranged by size:
881 358 347 315 299 241 209 188
166 145 110 94 93 93 80 79
58 33 18
BspEI T'CCGG_A
Cuts at: 0 280 3807
Size: 280 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 533 620 650 1001 1320 1764 2475
Size: 149 384 87 30 351 319 444 711
Cuts at: 2475 2804 3384 3807
Size: 329 580 423
Fragments arranged by size:
711 580 444 423 384 351 329 319
149 87 30
BsrBI CCG'CTC
Cuts at: 0 2049 3711 3807
Size: 2049 1662 96
Fragments arranged by size:
2049 1662 96
BsrDI GCAATG_nn'
Cuts at: 0 702 2550 3750 3807
Size: 702 1848 1200 57
Fragments arranged by size:
1848 1200 702 57
BsrFI r'CCGG_y
Cuts at: 0 2435 3719 3807
Size: 2435 1284 88
Fragments arranged by size:
2435 1284 88
BsrGI T'GTAC_A
Cuts at: 0 725 2183 2324 3807
Size: 725 1458 141 1483
Fragments arranged by size:
1483 1458 725 141
BssHII G'CGCG_C
Cuts at: 0 243 2388 3807
Size: 243 2145 1419
Fragments arranged by size:
2145 1419 243
BssSI C'ACGA_G
Cuts at: 0 535 3807
Size: 535 3272
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 1153 2304 3206 3807
Size: 606 547 1151 902 601
Fragments arranged by size:
1151 902 606 601 547
BstEII G'GTnAC_C
Cuts at: 0 1056 3807
Size: 1056 2751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 654 909 2105 2667 3807
Size: 654 255 1196 562 1140
Fragments arranged by size:
1196 1140 654 562 255
BstYI r'GATC_y
Cuts at: 0 684 1631 3173 3807
Size: 684 947 1542 634
Fragments arranged by size:
1542 947 684 634
Bsu36I CC'TnA_GG
Cuts at: 0 930 3807
Size: 930 2877
BtgI C'CryG_G
Cuts at: 0 358 1226 1554 2243 2450 2647 3807
Size: 358 868 328 689 207 197 1160
Fragments arranged by size:
1160 868 689 358 328 207 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 221 601 1766 3807
Size: 221 380 1165 2041
Fragments arranged by size:
2041 1165 380 221
BtsI GCAGTG_nn'
Cuts at: 0 847 2071 2630 3536 3807
Size: 847 1224 559 906 271
Fragments arranged by size:
1224 906 847 559 271
DraIII CAC_nnn'GTG
Cuts at: 0 1056 2119 3206 3807
Size: 1056 1063 1087 601
Fragments arranged by size:
1087 1063 1056 601
DrdI GACnn_nn'nnGTC
Cuts at: 0 2800 3807
Size: 2800 1007
EaeI y'GGCC_r
Cuts at: 0 155 2164 2364 2437 2669 2775 3600 3712
Size: 155 2009 200 73 232 106 825 112
Cuts at: 3712 3807
Size: 95
Fragments arranged by size:
2009 825 232 200 155 112 106 95
73
EagI C'GGCC_G
Cuts at: 0 2364 3712 3807
Size: 2364 1348 95
Fragments arranged by size:
2364 1348 95
EarI CTCTTCn'nnn_
Cuts at: 0 442 953 2759 3037 3502 3807
Size: 442 511 1806 278 465 305
Fragments arranged by size:
1806 511 465 442 305 278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2692 3807
Size: 2692 1115
EcoICRI GAG'CTC
Cuts at: 0 2094 3807
Size: 2094 1713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 292 388 508 676 901 1006 1357
Size: 133 159 96 120 168 225 105 351
Cuts at: 1357 1459 2174 2233 2755 2988 3067 3213 3682
Size: 102 715 59 522 233 79 146 469
Cuts at: 3682 3801 3807
Size: 119 6
Fragments arranged by size:
715 522 469 351 233 225 168 159
146 133 120 119 105 102 96 79
59 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 3807
Size: 145 3662
EcoO109I rG'GnC_Cy
Cuts at: 0 1098 1263 1401 1434 1916 1982 3626 3798
Size: 1098 165 138 33 482 66 1644 172
Cuts at: 3798 3807
Size: 9
Fragments arranged by size:
1644 1098 482 172 165 138 66 33
9
EcoRI G'AATT_C
Cuts at: 0 276 897 3807
Size: 276 621 2910
Fragments arranged by size:
2910 621 276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 3288 3540 3807
Size: 930 2358 252 267
Fragments arranged by size:
2358 930 267 252
FspI TGC'GCA
Cuts at: 0 1705 3807
Size: 1705 2102
HaeII r_GCGC'y
Cuts at: 0 3152 3194 3788 3807
Size: 3152 42 594 19
Fragments arranged by size:
3152 594 42 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 1490 1522 1600 1632 2326 2358
Size: 301 32 1157 32 78 32 694 32
Cuts at: 2358 2556 2588 3807
Size: 198 32 1219
Fragments arranged by size:
1219 1157 694 301 198 78 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1698 2525 3807
Size: 1698 827 1282
Fragments arranged by size:
1698 1282 827
HindIII A'AGCT_T
Cuts at: 0 1673 3807
Size: 1673 2134
KasI G'GCGC_C
Cuts at: 0 3148 3807
Size: 3148 659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1874 2369 3533 3572 3807
Size: 926 948 495 1164 39 235
Fragments arranged by size:
1164 948 926 495 235 39
MscI TGG'CCA
Cuts at: 0 2777 3602 3807
Size: 2777 825 205
Fragments arranged by size:
2777 825 205
MslI CAynn'nnrTG
Cuts at: 0 13 388 1858 3619 3807
Size: 13 375 1470 1761 188
Fragments arranged by size:
1761 1470 375 188 13
MspA1I CmG'CkG
Cuts at: 0 47 345 360 1159 1197 1237 1243 1409
Size: 47 298 15 799 38 40 6 166
Cuts at: 1409 1491 1613 2649 2685 2905 3767 3807
Size: 82 122 1036 36 220 862 40
Fragments arranged by size:
1036 862 799 298 220 166 122 82
47 40 40 38 36 15 6
NaeI GCC'GGC
Cuts at: 0 3721 3807
Size: 3721 86
NarI GG'CG_CC
Cuts at: 0 3149 3807
Size: 3149 658
NgoMIV G'CCGG_C
Cuts at: 0 3719 3807
Size: 3719 88
NheI G'CTAG_C
Cuts at: 0 1203 3807
Size: 1203 2604
NotI GC'GGCC_GC
Cuts at: 0 3712 3807
Size: 3712 95
NspI r_CATG'y
Cuts at: 0 387 565 1347 3618 3693 3807
Size: 387 178 782 2271 75 114
Fragments arranged by size:
2271 782 387 178 114 75
PasI CC'CwG_GG
Cuts at: 0 3037 3807
Size: 3037 770
PciI A'CATG_T
Cuts at: 0 3614 3807
Size: 3614 193
PflMI CCAn_nnn'nTGG
Cuts at: 0 1437 3807
Size: 1437 2370
PfoI T'CCnGG_A
Cuts at: 0 323 1100 1736 2578 3807
Size: 323 777 636 842 1229
Fragments arranged by size:
1229 842 777 636 323
PmlI CAC'GTG
Cuts at: 0 810 2148 3807
Size: 810 1338 1659
Fragments arranged by size:
1659 1338 810
PpuMI rG'GwC_Cy
Cuts at: 0 1098 1263 1916 3626 3807
Size: 1098 165 653 1710 181
Fragments arranged by size:
1710 1098 653 181 165
PspOMI G'GGCC_C
Cuts at: 0 1255 1400 1982 3807
Size: 1255 145 582 1825
Fragments arranged by size:
1825 1255 582 145
PspXI vC'TCGA_Gb
Cuts at: 0 2467 3807
Size: 2467 1340
PstI C_TGCA'G
Cuts at: 0 167 530 602 1012 1190 1236 1242 1248
Size: 167 363 72 410 178 46 6 6
Cuts at: 1248 1775 2300 2564 2815 3807
Size: 527 525 264 251 992
Fragments arranged by size:
992 527 525 410 363 264 251 178
167 72 46 6 6
PvuII CAG'CTG
Cuts at: 0 345 1197 1237 1243 1491 2685 3807
Size: 345 852 40 6 248 1194 1122
Fragments arranged by size:
1194 1122 852 345 248 40 6
SacI G_AGCT'C
Cuts at: 0 2096 3807
Size: 2096 1711
SacII CC_GC'GG
Cuts at: 0 361 2650 3807
Size: 361 2289 1157
Fragments arranged by size:
2289 1157 361
SanDI GG'GwC_CC
Cuts at: 0 3626 3807
Size: 3626 181
SapI GCTCTTCn'nnn_
Cuts at: 0 442 3807
Size: 442 3365
SbfI CC_TGCA'GG
Cuts at: 0 1775 3807
Size: 1775 2032
SexAI A'CCwGG_T
Cuts at: 0 1052 1124 3807
Size: 1052 72 2683
Fragments arranged by size:
2683 1052 72
SfcI C'TryA_G
Cuts at: 0 163 526 598 1008 1186 1232 1238 1244
Size: 163 363 72 410 178 46 6 6
Cuts at: 1244 1771 2296 2560 2811 3384 3807
Size: 527 525 264 251 573 423
Fragments arranged by size:
573 527 525 423 410 363 264 251
178 163 72 46 6 6
SfoI GGC'GCC
Cuts at: 0 3150 3807
Size: 3150 657
SmaI CCC'GGG
Cuts at: 0 1399 2570 3807
Size: 1399 1171 1237
Fragments arranged by size:
1399 1237 1171
SmlI C'TyrA_G
Cuts at: 0 330 1591 1909 2401 2467 3262 3807
Size: 330 1261 318 492 66 795 545
Fragments arranged by size:
1261 795 545 492 330 318 66
SnaBI TAC'GTA
Cuts at: 0 1971 3807
Size: 1971 1836
SphI G_CATG'C
Cuts at: 0 565 3807
Size: 565 3242
SrfI GCCC'GGGC
Cuts at: 0 1399 3807
Size: 1399 2408
StuI AGG'CCT
Cuts at: 0 3667 3807
Size: 3667 140
StyI C'CwwG_G
Cuts at: 0 1259 1930 2009 2264 3698 3807
Size: 1259 671 79 255 1434 109
Fragments arranged by size:
1434 1259 671 255 109 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 637 646 792 1705 1967 2107 2811 3300
Size: 637 9 146 913 262 140 704 489
Cuts at: 3300 3423 3609 3807
Size: 123 186 198
Fragments arranged by size:
913 704 637 489 262 198 186 146
140 123 9
TatI w'GTAC_w
Cuts at: 0 725 2183 2324 2951 3476 3807
Size: 725 1458 141 627 525 331
Fragments arranged by size:
1458 725 627 525 331 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1851 1883 2926 2958 3482 3514 3679 3711
Size: 1851 32 1043 32 524 32 165 32
Cuts at: 3711 3807
Size: 96
Fragments arranged by size:
1851 1043 524 165 96 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 981 1266 3807
Size: 384 597 285 2541
Fragments arranged by size:
2541 597 384 285
XhoI C'TCGA_G
Cuts at: 0 2467 3807
Size: 2467 1340
XmaI C'CCGG_G
Cuts at: 0 1397 2568 3807
Size: 1397 1171 1239
Fragments arranged by size:
1397 1239 1171
XmnI GAAnn'nnTTC
Cuts at: 0 3427 3807
Size: 3427 380
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI
SnaBI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BsaBI BsiWI BspHI BstBI BstZ17I ClaI
CspCI DraI EcoRV FseI FspAI HpaI KpnI MfeI
MluI NcoI NdeI NruI NsiI PacI PmeI PpiI
PshAI PsiI PsrI PvuI RsrII SalI ScaI SfiI
SgfI SgrAI SpeI SspI SwaI Tth111I XbaI ZraI