Restriction map for
pFN21AE1456
(Linear) (Six-base) MAPSORT of: KIEE1456.seq Check: 7500 from: 1 to: 510
KIEE1456.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AatII G_ACGT'C
Cuts at: 0 182 510
Size: 182 328
Acc65I G'GTAC_C
Cuts at: 0 120 510
Size: 120 390
AflIII A'CryG_T
Cuts at: 0 212 510
Size: 212 298
AhdI GACnn_n'nnGTC
Cuts at: 0 183 510
Size: 183 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 346 378 510
Size: 199 32 115 32 132
Fragments arranged by size:
199 132 115 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 39 152 510
Size: 39 113 358
Fragments arranged by size:
358 113 39
BanI G'GyrC_C
Cuts at: 0 120 510
Size: 120 390
BglI GCCn_nnn'nGGC
Cuts at: 0 49 510
Size: 49 461
Bme1580I G_kGCm'C
Cuts at: 0 68 510
Size: 68 442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 510
Size: 184 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 420 459 510
Size: 420 39 51
Fragments arranged by size:
420 51 39
BsaAI yAC'GTr
Cuts at: 0 351 510
Size: 351 159
BsaBI GATnn'nnATC
Cuts at: 0 269 510
Size: 269 241
BsaHI Gr'CG_yC
Cuts at: 0 179 510
Size: 179 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 328 346 358 376 510
Size: 199 30 99 18 12 18 134
Fragments arranged by size:
199 134 99 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 462 465 510
Size: 438 24 3 45
Fragments arranged by size:
438 45 24 3
BseYI C'CCAG_C
Cuts at: 0 71 91 196 510
Size: 71 20 105 314
Fragments arranged by size:
314 105 71 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 259 510
Size: 231 28 251
Fragments arranged by size:
251 231 28
BsiHKAI G_wGCw'C
Cuts at: 0 212 510
Size: 212 298
BsmI GAATG_Cn'
Cuts at: 0 391 510
Size: 391 119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 286 510
Size: 286 224
Bsp1286I G_dGCh'C
Cuts at: 0 68 212 510
Size: 68 144 298
Fragments arranged by size:
298 144 68
BspHI T'CATG_A
Cuts at: 0 8 510
Size: 8 502
BsrFI r'CCGG_y
Cuts at: 0 3 250 285 338 389 510
Size: 3 247 35 53 51 121
Fragments arranged by size:
247 121 53 51 35 3
BstAPI GCAn_nnn'nTGC
Cuts at: 0 152 254 282 510
Size: 152 102 28 228
Fragments arranged by size:
228 152 102 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 510
Size: 204 306
BstYI r'GATC_y
Cuts at: 0 323 510
Size: 323 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 278 506 510
Size: 278 228 4
Fragments arranged by size:
278 228 4
BtsI GCAGTG_nn'
Cuts at: 0 82 510
Size: 82 428
DraIII CAC_nnn'GTG
Cuts at: 0 282 510
Size: 282 228
EaeI y'GGCC_r
Cuts at: 0 41 296 510
Size: 41 255 214
Fragments arranged by size:
255 214 41
EarI CTCTTCn'nnn_
Cuts at: 0 439 510
Size: 439 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 510
Size: 184 326
HaeII r_GCGC'y
Cuts at: 0 55 163 510
Size: 55 108 347
Fragments arranged by size:
347 108 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 328 351 360 383 510
Size: 169 32 127 23 9 23 127
Fragments arranged by size:
169 127 127 32 23 23 9
KpnI G_GTAC'C
Cuts at: 0 124 510
Size: 124 386
NaeI GCC'GGC
Cuts at: 0 391 510
Size: 391 119
NgoMIV G'CCGG_C
Cuts at: 0 389 510
Size: 389 121
NspI r_CATG'y
Cuts at: 0 216 510
Size: 216 294
PciI A'CATG_T
Cuts at: 0 212 510
Size: 212 298
PfoI T'CCnGG_A
Cuts at: 0 128 510
Size: 128 382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 346 378 510
Size: 199 32 115 32 132
Fragments arranged by size:
199 132 115 32 32
PstI C_TGCA'G
Cuts at: 0 35 510
Size: 35 475
SfcI C'TryA_G
Cuts at: 0 31 510
Size: 31 479
StyI C'CwwG_G
Cuts at: 0 431 510
Size: 431 79
ZraI GAC'GTC
Cuts at: 0 180 510
Size: 180 330
Enzymes that do cut:
AatII Acc65I AflIII AhdI AloI AlwNI BanI BglI
Bme1580I BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI
BstAPI BstXI BstYI BtgZI BtsI DraIII EaeI EarI
Eco57MI HaeII Hin4I KpnI NaeI NgoMIV NspI PciI
PfoI PpiI PstI SfcI StyI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AgeI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaWI BsiEI BsiWI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI