(Linear) (Six-base) MAPSORT of: KIEE1455.seq  Check: 8207  from: 1  to: 300



KIEE1455.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     300 
   Size:        119     181
 
AlwNI CAG_nnn'CTG

Cuts at:      0     265     300 
   Size:        265      35
 
BanI G'GyrC_C

Cuts at:      0      31     300 
   Size:         31     269
 
BanII G_rGCy'C

Cuts at:      0      50     300 
   Size:         50     250
 
BbeI G_GCGC'C

Cuts at:      0      35     300 
   Size:         35     265
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     221     250     300 
   Size:        221      29      50

  Fragments arranged by size: 

                 221      50      29
 
BglI GCCn_nnn'nGGC

Cuts at:      0     193     300 
   Size:        193     107
 
BsaHI Gr'CG_yC

Cuts at:      0      32     300 
   Size:         32     268
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69     265     300 
   Size:         69     196      35

  Fragments arranged by size: 

                 196      69      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     300 
   Size:         50     250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     231     300 
   Size:        231      69
 
EarI CTCTTCn'nnn_

Cuts at:      0      38     300 
   Size:         38     262
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      12     300 
   Size:         12     288
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     300 
   Size:        119     181
 
HaeII r_GCGC'y

Cuts at:      0      35     300 
   Size:         35     265
 
KasI G'GCGC_C

Cuts at:      0      31     300 
   Size:         31     269
 
NarI GG'CG_CC

Cuts at:      0      32     300 
   Size:         32     268
 
SapI GCTCTTCn'nnn_

Cuts at:      0      38     300 
   Size:         38     262
 
SfoI GGC'GCC

Cuts at:      0      33     300 
   Size:         33     267
 
StyI C'CwwG_G

Cuts at:      0      38     300 
   Size:         38     262
 

 Enzymes that do cut:

     AcuI    AlwNI     BanI    BanII     BbeI     BbsI     BglI    BsaHI
    BseRI Bsp1286I    BspMI     EarI     EciI  Eco57MI    HaeII     KasI
     NarI     SapI     SfoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI