Restriction map for
pFN21AE1454
(Linear) (Six-base) MAPSORT of: KIEE1454.seq Check: 499 from: 1 to: 1950
KIEE1454.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 563 1950
Size: 563 1387
AatII G_ACGT'C
Cuts at: 0 1461 1950
Size: 1461 489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1838 1950
Size: 781 1057 112
Fragments arranged by size:
1057 781 112
AleI CACnn'nnGTG
Cuts at: 0 1232 1950
Size: 1232 718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 776 808 1950
Size: 776 32 1142
Fragments arranged by size:
1142 776 32
AlwNI CAG_nnn'CTG
Cuts at: 0 109 1519 1950
Size: 109 1410 431
Fragments arranged by size:
1410 431 109
ApaI G_GGCC'C
Cuts at: 0 49 556 856 1000 1276 1907 1950
Size: 49 507 300 144 276 631 43
Fragments arranged by size:
631 507 300 276 144 49 43
ApaLI G'TGCA_C
Cuts at: 0 896 1950
Size: 896 1054
ApoI r'AATT_y
Cuts at: 0 1141 1750 1950
Size: 1141 609 200
Fragments arranged by size:
1141 609 200
AvaI C'yCGr_G
Cuts at: 0 31 564 1950
Size: 31 533 1386
Fragments arranged by size:
1386 533 31
AvrII C'CTAG_G
Cuts at: 0 657 1708 1950
Size: 657 1051 242
Fragments arranged by size:
1051 657 242
BamHI G'GATC_C
Cuts at: 0 215 1347 1950
Size: 215 1132 603
Fragments arranged by size:
1132 603 215
BanI G'GyrC_C
Cuts at: 0 71 1370 1950
Size: 71 1299 580
Fragments arranged by size:
1299 580 71
BanII G_rGCy'C
Cuts at: 0 49 556 565 650 830 856 1000 1207
Size: 49 507 9 85 180 26 144 207
Cuts at: 1207 1250 1276 1535 1654 1907 1950
Size: 43 26 259 119 253 43
Fragments arranged by size:
507 259 253 207 180 144 119 85
49 43 43 26 26 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1670 1950
Size: 1670 280
BbvCI CC'TCA_GC
Cuts at: 0 1164 1950
Size: 1164 786
BclI T'GATC_A
Cuts at: 0 113 1950
Size: 113 1837
BglI GCCn_nnn'nGGC
Cuts at: 0 1939 1950
Size: 1939 11
Bme1580I G_kGCm'C
Cuts at: 0 49 74 556 856 900 1000 1276 1907
Size: 49 25 482 300 44 100 276 631
Cuts at: 1907 1922 1950
Size: 15 28
Fragments arranged by size:
631 482 300 276 100 49 44 28
25 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 1226 1950
Size: 626 600 724
Fragments arranged by size:
724 626 600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 997 1950
Size: 997 953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 412 664 1264 1686 1895 1950
Size: 361 51 252 600 422 209 55
Fragments arranged by size:
600 422 361 252 209 55 51
Bpu10I CC'TnA_GC
Cuts at: 0 1164 1950
Size: 1164 786
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 629 712 1260 1950
Size: 154 475 83 548 690
Fragments arranged by size:
690 548 475 154 83
BsaAI yAC'GTr
Cuts at: 0 60 1950
Size: 60 1890
BsaBI GATnn'nnATC
Cuts at: 0 1390 1950
Size: 1390 560
BsaHI Gr'CG_yC
Cuts at: 0 1101 1458 1950
Size: 1101 357 492
Fragments arranged by size:
1101 492 357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 892 895 922 925 961 991
Size: 404 30 458 3 27 3 36 30
Cuts at: 991 1007 1037 1403 1433 1869 1899 1950
Size: 16 30 366 30 436 30 51
Fragments arranged by size:
458 436 404 366 51 36 30 30
30 30 30 27 16 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 730 900 1408 1411 1414 1417 1420 1423
Size: 730 170 508 3 3 3 3 3
Cuts at: 1423 1718 1877 1950
Size: 295 159 73
Fragments arranged by size:
730 508 295 170 159 73 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 386 391 509 823 928 1276 1566 1844
Size: 386 5 118 314 105 348 290 278
Cuts at: 1844 1921 1950
Size: 77 29
Fragments arranged by size:
386 348 314 290 278 118 105 77
29 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 709 1009 1834 1950
Size: 82 627 300 825 116
Fragments arranged by size:
825 627 300 116 82
BsiHKAI G_wGCw'C
Cuts at: 0 650 900 1207 1250 1950
Size: 650 250 307 43 700
Fragments arranged by size:
700 650 307 250 43
Bsp1286I G_dGCh'C
Cuts at: 0 49 74 556 565 650 830 856 900
Size: 49 25 482 9 85 180 26 44
Cuts at: 900 1000 1207 1250 1276 1535 1654 1907 1922
Size: 100 207 43 26 259 119 253 15
Cuts at: 1922 1950
Size: 28
Fragments arranged by size:
482 259 253 207 180 119 100 85
49 44 43 28 26 26 25 15
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 563 1226 1950
Size: 563 663 724
Fragments arranged by size:
724 663 563
BsrBI CCG'CTC
Cuts at: 0 320 866 1950
Size: 320 546 1084
Fragments arranged by size:
1084 546 320
BssSI C'ACGA_G
Cuts at: 0 1249 1950
Size: 1249 701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 399 523 1950
Size: 399 124 1427
Fragments arranged by size:
1427 399 124
BstYI r'GATC_y
Cuts at: 0 215 1347 1950
Size: 215 1132 603
Fragments arranged by size:
1132 603 215
Bsu36I CC'TnA_GG
Cuts at: 0 139 713 1011 1316 1622 1950
Size: 139 574 298 305 306 328
Fragments arranged by size:
574 328 306 305 298 139
BtgI C'CryG_G
Cuts at: 0 398 522 548 1001 1096 1148 1950
Size: 398 124 26 453 95 52 802
Fragments arranged by size:
802 453 398 124 95 52 26
BtsI GCAGTG_nn'
Cuts at: 0 308 336 739 1558 1682 1950
Size: 308 28 403 819 124 268
Fragments arranged by size:
819 403 308 268 124 28
DrdI GACnn_nn'nnGTC
Cuts at: 0 1457 1950
Size: 1457 493
EaeI y'GGCC_r
Cuts at: 0 1400 1940 1950
Size: 1400 540 10
Fragments arranged by size:
1400 540 10
EarI CTCTTCn'nnn_
Cuts at: 0 1441 1775 1950
Size: 1441 334 175
Fragments arranged by size:
1441 334 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1792 1950
Size: 1792 158
EcoICRI GAG'CTC
Cuts at: 0 648 1205 1248 1950
Size: 648 557 43 702
Fragments arranged by size:
702 648 557 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 412 664 781 1264 1686 1838 1895
Size: 361 51 252 117 483 422 152 57
Cuts at: 1895 1950
Size: 55
Fragments arranged by size:
483 422 361 252 152 117 57 55
51
EcoO109I rG'GnC_Cy
Cuts at: 0 46 553 697 853 997 1153 1178 1272
Size: 46 507 144 156 144 156 25 94
Cuts at: 1272 1273 1294 1903 1904 1950
Size: 1 21 609 1 46
Fragments arranged by size:
609 507 156 156 144 144 94 46
46 25 21 1 1
EcoRI G'AATT_C
Cuts at: 0 1141 1750 1950
Size: 1141 609 200
Fragments arranged by size:
1141 609 200
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 350 382 552 584 592 624 711 743
Size: 350 32 170 32 8 32 87 32
Cuts at: 743 961 993 1629 1661 1950
Size: 218 32 636 32 289
Fragments arranged by size:
636 350 289 218 170 87 32 32
32 32 32 32 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 1473 1527 1950
Size: 1325 148 54 423
Fragments arranged by size:
1325 423 148 54
MscI TGG'CCA
Cuts at: 0 1942 1950
Size: 1942 8
MslI CAynn'nnrTG
Cuts at: 0 521 1047 1232 1396 1950
Size: 521 526 185 164 554
Fragments arranged by size:
554 526 521 185 164
MspA1I CmG'CkG
Cuts at: 0 1087 1570 1848 1915 1950
Size: 1087 483 278 67 35
Fragments arranged by size:
1087 483 278 67 35
NcoI C'CATG_G
Cuts at: 0 1096 1148 1950
Size: 1096 52 802
Fragments arranged by size:
1096 802 52
NspI r_CATG'y
Cuts at: 0 277 1950
Size: 277 1673
PasI CC'CwG_GG
Cuts at: 0 120 849 1950
Size: 120 729 1101
Fragments arranged by size:
1101 729 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 92 266 1744 1950
Size: 92 174 1478 206
Fragments arranged by size:
1478 206 174 92
PfoI T'CCnGG_A
Cuts at: 0 641 1754 1950
Size: 641 1113 196
Fragments arranged by size:
1113 641 196
PmlI CAC'GTG
Cuts at: 0 60 1950
Size: 60 1890
PpuMI rG'GwC_Cy
Cuts at: 0 697 1153 1178 1950
Size: 697 456 25 772
Fragments arranged by size:
772 697 456 25
PspOMI G'GGCC_C
Cuts at: 0 45 552 852 996 1272 1903 1950
Size: 45 507 300 144 276 631 47
Fragments arranged by size:
631 507 300 276 144 47 45
PstI C_TGCA'G
Cuts at: 0 315 734 1515 1553 1950
Size: 315 419 781 38 397
Fragments arranged by size:
781 419 397 315 38
PvuII CAG'CTG
Cuts at: 0 1087 1570 1848 1915 1950
Size: 1087 483 278 67 35
Fragments arranged by size:
1087 483 278 67 35
SacI G_AGCT'C
Cuts at: 0 650 1207 1250 1950
Size: 650 557 43 700
Fragments arranged by size:
700 650 557 43
SbfI CC_TGCA'GG
Cuts at: 0 1515 1950
Size: 1515 435
SfcI C'TryA_G
Cuts at: 0 311 730 1182 1511 1549 1950
Size: 311 419 452 329 38 401
Fragments arranged by size:
452 419 401 329 311 38
StuI AGG'CCT
Cuts at: 0 1547 1950
Size: 1547 403
StyI C'CwwG_G
Cuts at: 0 657 1073 1096 1148 1708 1950
Size: 657 416 23 52 560 242
Fragments arranged by size:
657 560 416 242 52 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 506 1950
Size: 133 373 1444
Fragments arranged by size:
1444 373 133
TatI w'GTAC_w
Cuts at: 0 881 1950
Size: 881 1069
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 892 924 1007 1039 1950
Size: 892 32 83 32 911
Fragments arranged by size:
911 892 83 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 973 1950
Size: 973 977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 263 546 639 846 936 1239 1950
Size: 263 283 93 207 90 303 711
Fragments arranged by size:
711 303 283 263 207 93 90
ZraI GAC'GTC
Cuts at: 0 1459 1950
Size: 1459 491
Enzymes that do cut:
AarI AatII AcuI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BssSI BstXI BstYI Bsu36I BtgI
BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I MmeI MscI MslI MspA1I NcoI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SbfI SfcI StuI StyI TaqII TatI TstI
Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AscI AseI AsiSI BaeI BbeI BcgI BciVI
BfrBI BglII BlpI BmgBI BmtI BpuEI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
XmnI