Restriction map for
pFN21AE1451
(Linear) (Six-base) MAPSORT of: KIEE1451.seq Check: 1393 from: 1 to: 1077
KIEE1451.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 427 978 1077
Size: 427 551 99
Fragments arranged by size:
551 427 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1077
Size: 754 323
AfeI AGC'GCT
Cuts at: 0 997 1077
Size: 997 80
AlwNI CAG_nnn'CTG
Cuts at: 0 57 1077
Size: 57 1020
ApaLI G'TGCA_C
Cuts at: 0 1044 1077
Size: 1044 33
AvaI C'yCGr_G
Cuts at: 0 109 154 458 533 666 1077
Size: 109 45 304 75 133 411
Fragments arranged by size:
411 304 133 109 75 45
BanI G'GyrC_C
Cuts at: 0 127 1077
Size: 127 950
BanII G_rGCy'C
Cuts at: 0 51 161 413 886 947 1077
Size: 51 110 252 473 61 130
Fragments arranged by size:
473 252 130 110 61 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1035 1069 1077
Size: 1035 34 8
Fragments arranged by size:
1035 34 8
BglI GCCn_nnn'nGGC
Cuts at: 0 404 1077
Size: 404 673
BlpI GC'TnA_GC
Cuts at: 0 50 1077
Size: 50 1027
Bme1580I G_kGCm'C
Cuts at: 0 132 895 1048 1077
Size: 132 763 153 29
Fragments arranged by size:
763 153 132 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1077
Size: 808 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1077
Size: 667 410
BsaI GGTCTCn'nnnn_
Cuts at: 0 99 331 1077
Size: 99 232 746
Fragments arranged by size:
746 232 99
BsaBI GATnn'nnATC
Cuts at: 0 118 1077
Size: 118 959
BsaHI Gr'CG_yC
Cuts at: 0 65 662 1077
Size: 65 597 415
Fragments arranged by size:
597 415 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 1077
Size: 55 30 992
Fragments arranged by size:
992 55 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 583 1077
Size: 583 494
BseYI C'CCAG_C
Cuts at: 0 4 44 131 812 847 1077
Size: 4 40 87 681 35 230
Fragments arranged by size:
681 230 87 40 35 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 162 1006 1077
Size: 121 41 844 71
Fragments arranged by size:
844 121 71 41
BsiEI CG_ry'CG
Cuts at: 0 615 758 1077
Size: 615 143 319
Fragments arranged by size:
615 319 143
BsiHKAI G_wGCw'C
Cuts at: 0 203 413 587 880 947 1048 1077
Size: 203 210 174 293 67 101 29
Fragments arranged by size:
293 210 203 174 101 67 29
BsmI GAATG_Cn'
Cuts at: 0 250 1077
Size: 250 827
BsmBI CGTCTCn'nnnn_
Cuts at: 0 669 1077
Size: 669 408
Bsp1286I G_dGCh'C
Cuts at: 0 51 132 161 203 413 587 880 886
Size: 51 81 29 42 210 174 293 6
Cuts at: 886 895 947 1048 1077
Size: 9 52 101 29
Fragments arranged by size:
293 210 174 101 81 52 51 42
29 29 9 6
BspHI T'CATG_A
Cuts at: 0 587 1077
Size: 587 490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 461 602 697 978 1077
Size: 427 34 141 95 281 99
Fragments arranged by size:
427 281 141 99 95 34
BsrFI r'CCGG_y
Cuts at: 0 610 728 782 1077
Size: 610 118 54 295
Fragments arranged by size:
610 295 118 54
BtgI C'CryG_G
Cuts at: 0 365 657 1077
Size: 365 292 420
Fragments arranged by size:
420 365 292
BtsI GCAGTG_nn'
Cuts at: 0 1002 1077
Size: 1002 75
DraIII CAC_nnn'GTG
Cuts at: 0 357 1077
Size: 357 720
EaeI y'GGCC_r
Cuts at: 0 168 704 755 1077
Size: 168 536 51 322
Fragments arranged by size:
536 322 168 51
EagI C'GGCC_G
Cuts at: 0 755 1077
Size: 755 322
EcoICRI GAG'CTC
Cuts at: 0 411 945 1077
Size: 411 534 132
Fragments arranged by size:
534 411 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 808 1077
Size: 754 54 269
Fragments arranged by size:
754 269 54
EcoO109I rG'GnC_Cy
Cuts at: 0 450 671 828 1077
Size: 450 221 157 249
Fragments arranged by size:
450 249 221 157
FspI TGC'GCA
Cuts at: 0 868 1077
Size: 868 209
HaeII r_GCGC'y
Cuts at: 0 365 999 1077
Size: 365 634 78
Fragments arranged by size:
634 365 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 283 315 1077
Size: 55 32 196 32 762
Fragments arranged by size:
762 196 55 32 32
HincII GTy'rAC
Cuts at: 0 378 798 1077
Size: 378 420 279
Fragments arranged by size:
420 378 279
MfeI C'AATT_G
Cuts at: 0 187 1077
Size: 187 890
MscI TGG'CCA
Cuts at: 0 170 1077
Size: 170 907
MspA1I CmG'CkG
Cuts at: 0 44 267 289 367 702 1077
Size: 44 223 22 78 335 375
Fragments arranged by size:
375 335 223 78 44 22
NaeI GCC'GGC
Cuts at: 0 784 1077
Size: 784 293
NgoMIV G'CCGG_C
Cuts at: 0 782 1077
Size: 782 295
NotI GC'GGCC_GC
Cuts at: 0 755 1077
Size: 755 322
PasI CC'CwG_GG
Cuts at: 0 888 1077
Size: 888 189
PflMI CCAn_nnn'nTGG
Cuts at: 0 74 1077
Size: 74 1003
PpuMI rG'GwC_Cy
Cuts at: 0 450 671 828 1077
Size: 450 221 157 249
Fragments arranged by size:
450 249 221 157
PstI C_TGCA'G
Cuts at: 0 430 679 701 1077
Size: 430 249 22 376
Fragments arranged by size:
430 376 249 22
PvuII CAG'CTG
Cuts at: 0 44 702 1077
Size: 44 658 375
Fragments arranged by size:
658 375 44
SacI G_AGCT'C
Cuts at: 0 413 947 1077
Size: 413 534 130
Fragments arranged by size:
534 413 130
SacII CC_GC'GG
Cuts at: 0 368 1077
Size: 368 709
SexAI A'CCwGG_T
Cuts at: 0 830 1077
Size: 830 247
SfcI C'TryA_G
Cuts at: 0 426 675 697 1077
Size: 426 249 22 380
Fragments arranged by size:
426 380 249 22
SmaI CCC'GGG
Cuts at: 0 156 460 535 1077
Size: 156 304 75 542
Fragments arranged by size:
542 304 156 75
SmlI C'TyrA_G
Cuts at: 0 682 1077
Size: 682 395
StyI C'CwwG_G
Cuts at: 0 446 515 1077
Size: 446 69 562
Fragments arranged by size:
562 446 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 826 1077
Size: 364 462 251
Fragments arranged by size:
462 364 251
TatI w'GTAC_w
Cuts at: 0 1064 1077
Size: 1064 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 1077
Size: 454 32 591
Fragments arranged by size:
591 454 32
Tth111I GACn'n_nGTC
Cuts at: 0 832 1077
Size: 832 245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 1077
Size: 522 555
XmaI C'CCGG_G
Cuts at: 0 154 458 533 1077
Size: 154 304 75 544
Fragments arranged by size:
544 304 154 75
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaLI AvaI BanI BanII
BcgI BglI BlpI Bme1580I BpmI BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrFI BtgI BtsI DraIII
EaeI EagI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I
HincII MfeI MscI MspA1I NaeI NgoMIV NotI PasI
PflMI PpuMI PstI PvuII SacI SacII SexAI SfcI
SmaI SmlI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI
BsaWI BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI
MluI MmeI MslI NarI NcoI NdeI NheI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XhoI XmnI ZraI