Restriction map for
pFN21AE1450
(Linear) (Six-base) MAPSORT of: KIEE1450.seq Check: 7549 from: 1 to: 1284
KIEE1450.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 1284
Size: 912 372
AlwNI CAG_nnn'CTG
Cuts at: 0 1063 1284
Size: 1063 221
ApoI r'AATT_y
Cuts at: 0 422 1137 1284
Size: 422 715 147
Fragments arranged by size:
715 422 147
AseI AT'TA_AT
Cuts at: 0 184 1284
Size: 184 1100
BlpI GC'TnA_GC
Cuts at: 0 1078 1284
Size: 1078 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 430 741 1040 1284
Size: 424 6 311 299 244
Fragments arranged by size:
424 311 299 244 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1284
Size: 904 380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 756 1258 1284
Size: 262 494 502 26
Fragments arranged by size:
502 494 262 26
BseYI C'CCAG_C
Cuts at: 0 901 1073 1284
Size: 901 172 211
Fragments arranged by size:
901 211 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1284
Size: 1088 196
BsmI GAATG_Cn'
Cuts at: 0 398 746 1284
Size: 398 348 538
Fragments arranged by size:
538 398 348
BsmBI CGTCTCn'nnnn_
Cuts at: 0 648 1284
Size: 648 636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 1284
Size: 26 1258
BsrDI GCAATG_nn'
Cuts at: 0 647 1284
Size: 647 637
BsrGI T'GTAC_A
Cuts at: 0 84 1284
Size: 84 1200
BssSI C'ACGA_G
Cuts at: 0 1168 1284
Size: 1168 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1091 1284
Size: 1091 193
BstYI r'GATC_y
Cuts at: 0 627 1198 1284
Size: 627 571 86
Fragments arranged by size:
627 571 86
EaeI y'GGCC_r
Cuts at: 0 477 1284
Size: 477 807
EarI CTCTTCn'nnn_
Cuts at: 0 91 985 1284
Size: 91 894 299
Fragments arranged by size:
894 299 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 430 741 912 1040 1284
Size: 424 6 311 171 128 244
Fragments arranged by size:
424 311 244 171 128 6
EcoRI G'AATT_C
Cuts at: 0 422 1284
Size: 422 862
EcoRV GAT'ATC
Cuts at: 0 546 1284
Size: 546 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 1284
Size: 120 32 1132
Fragments arranged by size:
1132 120 32
HincII GTy'rAC
Cuts at: 0 608 1284
Size: 608 676
HpaI GTT'AAC
Cuts at: 0 608 1284
Size: 608 676
MfeI C'AATT_G
Cuts at: 0 41 1284
Size: 41 1243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1284
Size: 284 1000
MscI TGG'CCA
Cuts at: 0 479 1284
Size: 479 805
MslI CAynn'nnrTG
Cuts at: 0 959 1284
Size: 959 325
MspA1I CmG'CkG
Cuts at: 0 13 1284
Size: 13 1271
NspI r_CATG'y
Cuts at: 0 497 1284
Size: 497 787
PflMI CCAn_nnn'nTGG
Cuts at: 0 1063 1284
Size: 1063 221
PshAI GACnn'nnGTC
Cuts at: 0 660 1284
Size: 660 624
PstI C_TGCA'G
Cuts at: 0 695 1284
Size: 695 589
SfcI C'TryA_G
Cuts at: 0 581 691 1284
Size: 581 110 593
Fragments arranged by size:
593 581 110
SmlI C'TyrA_G
Cuts at: 0 883 1284
Size: 883 401
SspI AAT'ATT
Cuts at: 0 1005 1284
Size: 1005 279
StuI AGG'CCT
Cuts at: 0 731 1099 1284
Size: 731 368 185
Fragments arranged by size:
731 368 185
TatI w'GTAC_w
Cuts at: 0 84 452 1284
Size: 84 368 832
Fragments arranged by size:
832 368 84
XmnI GAAnn'nnTTC
Cuts at: 0 422 1284
Size: 422 862
Enzymes that do cut:
AcuI AlwNI ApoI AseI BlpI BpmI BpuEI BseRI
BseYI BsgI BsmI BsmBI BspMI BsrDI BsrGI BssSI
BstAPI BstYI EaeI EarI Eco57MI EcoRI EcoRV Hin4I
HincII HpaI MfeI MmeI MscI MslI MspA1I NspI
PflMI PshAI PstI SfcI SmlI SspI StuI TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I FalI
FseI FspI FspAI HaeII HindIII KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StyI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI