Restriction map for
pFN21AE1449
(Linear) (Six-base) MAPSORT of: KIEE1449.seq Check: 8016 from: 1 to: 1200
KIEE1449.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AccI GT'mk_AC
Cuts at: 0 469 1200
Size: 469 731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 252 1200
Size: 252 948
AlwNI CAG_nnn'CTG
Cuts at: 0 904 1200
Size: 904 296
ApaLI G'TGCA_C
Cuts at: 0 761 1200
Size: 761 439
ApoI r'AATT_y
Cuts at: 0 68 1200
Size: 68 1132
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 937 970 1200
Size: 937 33 230
Fragments arranged by size:
937 230 33
BanI G'GyrC_C
Cuts at: 0 894 1200
Size: 894 306
BanII G_rGCy'C
Cuts at: 0 696 1200
Size: 696 504
BbvCI CC'TCA_GC
Cuts at: 0 642 785 854 1200
Size: 642 143 69 346
Fragments arranged by size:
642 346 143 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 610 644 1200
Size: 610 34 556
Fragments arranged by size:
610 556 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 239 1200
Size: 239 961
BclI T'GATC_A
Cuts at: 0 567 1200
Size: 567 633
BglII A'GATC_T
Cuts at: 0 189 1200
Size: 189 1011
BlpI GC'TnA_GC
Cuts at: 0 938 1200
Size: 938 262
Bme1580I G_kGCm'C
Cuts at: 0 765 1200
Size: 765 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 1200
Size: 581 619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1200
Size: 398 802
Bpu10I CC'TnA_GC
Cuts at: 0 642 785 854 1200
Size: 642 143 69 346
Fragments arranged by size:
642 346 143 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1200
Size: 686 514
BsaBI GATnn'nnATC
Cuts at: 0 188 1200
Size: 188 1012
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1008 1038 1104 1134 1200
Size: 1008 30 66 30 66
Fragments arranged by size:
1008 66 66 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 627 630 1109 1200
Size: 627 3 479 91
Fragments arranged by size:
627 479 91 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 959 1200
Size: 258 701 241
Fragments arranged by size:
701 258 241
BsiHKAI G_wGCw'C
Cuts at: 0 594 696 765 1200
Size: 594 102 69 435
Fragments arranged by size:
594 435 102 69
Bsp1286I G_dGCh'C
Cuts at: 0 594 696 765 1200
Size: 594 102 69 435
Fragments arranged by size:
594 435 102 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 1200
Size: 697 503
BsrDI GCAATG_nn'
Cuts at: 0 626 1200
Size: 626 574
BsrFI r'CCGG_y
Cuts at: 0 751 1200
Size: 751 449
BstBI TT'CG_AA
Cuts at: 0 336 1200
Size: 336 864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 871 1200
Size: 871 329
BstYI r'GATC_y
Cuts at: 0 15 189 1200
Size: 15 174 1011
Fragments arranged by size:
1011 174 15
BtsI GCAGTG_nn'
Cuts at: 0 986 1200
Size: 986 214
DraI TTT'AAA
Cuts at: 0 512 1200
Size: 512 688
DraIII CAC_nnn'GTG
Cuts at: 0 589 1200
Size: 589 611
EaeI y'GGCC_r
Cuts at: 0 273 873 1200
Size: 273 600 327
Fragments arranged by size:
600 327 273
EcoICRI GAG'CTC
Cuts at: 0 694 1200
Size: 694 506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1200
Size: 398 802
EcoO109I rG'GnC_Cy
Cuts at: 0 388 1064 1200
Size: 388 676 136
Fragments arranged by size:
676 388 136
EcoRI G'AATT_C
Cuts at: 0 68 1200
Size: 68 1132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 64 1200
Size: 64 1136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 705 737 828 860 954 986 1104 1136
Size: 705 32 91 32 94 32 118 32
Cuts at: 1136 1200
Size: 64
Fragments arranged by size:
705 118 94 91 64 32 32 32
32
MscI TGG'CCA
Cuts at: 0 275 875 1200
Size: 275 600 325
Fragments arranged by size:
600 325 275
NaeI GCC'GGC
Cuts at: 0 753 1200
Size: 753 447
NgoMIV G'CCGG_C
Cuts at: 0 751 1200
Size: 751 449
PfoI T'CCnGG_A
Cuts at: 0 1098 1200
Size: 1098 102
PpuMI rG'GwC_Cy
Cuts at: 0 388 1200
Size: 388 812
PsiI TTA'TAA
Cuts at: 0 196 1200
Size: 196 1004
PstI C_TGCA'G
Cuts at: 0 600 1200
Size: 600 600
SacI G_AGCT'C
Cuts at: 0 696 1200
Size: 696 504
SexAI A'CCwGG_T
Cuts at: 0 1080 1188 1200
Size: 1080 108 12
Fragments arranged by size:
1080 108 12
SfcI C'TryA_G
Cuts at: 0 596 825 1200
Size: 596 229 375
Fragments arranged by size:
596 375 229
SmlI C'TyrA_G
Cuts at: 0 701 1200
Size: 701 499
SspI AAT'ATT
Cuts at: 0 402 1200
Size: 402 798
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 668 1200
Size: 600 68 532
Fragments arranged by size:
600 532 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 387 419 1200
Size: 387 32 781
Fragments arranged by size:
781 387 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1186 1200
Size: 1186 14
Enzymes that do cut:
AccI AlfI AlwNI ApaLI ApoI BaeI BanI BanII
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstBI BstXI BstYI BtsI
DraI DraIII EaeI EcoICRI Eco57MI EcoO109I EcoRI FalI
Hin4I MscI NaeI NgoMIV PfoI PpuMI PsiI PstI
SacI SexAI SfcI SmlI SspI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI AscI AseI AsiSI
AvaI AvrII BamHI BbeI BbsI BfrBI BglI BmgBI
BmtI BplI BsaI BsaAI BsaHI BsaWI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DrdI EagI EarI EciI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI