Restriction map for
pFN21AE1448
(Linear) (Six-base) MAPSORT of: KIEE1448.seq Check: 544 from: 1 to: 1089
KIEE1448.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 1089
Size: 15 1074
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1031 1089
Size: 929 102 58
Fragments arranged by size:
929 102 58
AlwNI CAG_nnn'CTG
Cuts at: 0 717 1089
Size: 717 372
ApaI G_GGCC'C
Cuts at: 0 635 1089
Size: 635 454
ApoI r'AATT_y
Cuts at: 0 184 1089
Size: 184 905
AvaI C'yCGr_G
Cuts at: 0 18 1089
Size: 18 1071
BanI G'GyrC_C
Cuts at: 0 3 1089
Size: 3 1086
BanII G_rGCy'C
Cuts at: 0 128 635 662 884 1014 1089
Size: 128 507 27 222 130 75
Fragments arranged by size:
507 222 130 128 75 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 655 1089
Size: 655 434
BfrBI ATG'CAT
Cuts at: 0 552 1089
Size: 552 537
BglI GCCn_nnn'nGGC
Cuts at: 0 832 1089
Size: 832 257
Bme1580I G_kGCm'C
Cuts at: 0 635 1089
Size: 635 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 118 119 805 1089
Size: 70 48 1 686 284
Fragments arranged by size:
686 284 70 48 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 697 1089
Size: 210 487 392
Fragments arranged by size:
487 392 210
BsaHI Gr'CG_yC
Cuts at: 0 15 1089
Size: 15 1074
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 922 1089
Size: 922 167
BseYI C'CCAG_C
Cuts at: 0 121 962 1089
Size: 121 841 127
Fragments arranged by size:
841 127 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1089
Size: 730 359
BsiHKAI G_wGCw'C
Cuts at: 0 461 662 1089
Size: 461 201 427
Fragments arranged by size:
461 427 201
Bsp1286I G_dGCh'C
Cuts at: 0 128 461 635 662 884 1014 1089
Size: 128 333 174 27 222 130 75
Fragments arranged by size:
333 222 174 130 128 75 27
BspHI T'CATG_A
Cuts at: 0 662 1089
Size: 662 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 1089
Size: 15 1074
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 1089
Size: 300 789
BstYI r'GATC_y
Cuts at: 0 335 759 1089
Size: 335 424 330
Fragments arranged by size:
424 335 330
BtgI C'CryG_G
Cuts at: 0 512 1089
Size: 512 577
BtsI GCAGTG_nn'
Cuts at: 0 149 1089
Size: 149 940
DrdI GACnn_nn'nnGTC
Cuts at: 0 1061 1089
Size: 1061 28
EarI CTCTTCn'nnn_
Cuts at: 0 97 1089
Size: 97 992
EcoICRI GAG'CTC
Cuts at: 0 660 1089
Size: 660 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 118 119 805 929 1031 1089
Size: 70 48 1 686 124 102 58
Fragments arranged by size:
686 124 102 70 58 48 1
EcoRI G'AATT_C
Cuts at: 0 184 1089
Size: 184 905
HaeII r_GCGC'y
Cuts at: 0 48 1089
Size: 48 1041
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 191 223 1089
Size: 191 32 866
Fragments arranged by size:
866 191 32
HincII GTy'rAC
Cuts at: 0 172 1089
Size: 172 917
MfeI C'AATT_G
Cuts at: 0 899 1089
Size: 899 190
MspA1I CmG'CkG
Cuts at: 0 962 1089
Size: 962 127
NsiI A_TGCA'T
Cuts at: 0 554 1089
Size: 554 535
NspI r_CATG'y
Cuts at: 0 552 984 1089
Size: 552 432 105
Fragments arranged by size:
552 432 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 806 995 1089
Size: 806 189 94
Fragments arranged by size:
806 189 94
PshAI GACnn'nnGTC
Cuts at: 0 786 1089
Size: 786 303
PspOMI G'GGCC_C
Cuts at: 0 631 1089
Size: 631 458
PvuII CAG'CTG
Cuts at: 0 962 1089
Size: 962 127
SacI G_AGCT'C
Cuts at: 0 662 1089
Size: 662 427
SmaI CCC'GGG
Cuts at: 0 20 1089
Size: 20 1069
SmlI C'TyrA_G
Cuts at: 0 189 676 1089
Size: 189 487 413
Fragments arranged by size:
487 413 189
StuI AGG'CCT
Cuts at: 0 835 1089
Size: 835 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 170 1089
Size: 170 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 1089
Size: 241 32 816
Fragments arranged by size:
816 241 32
XmaI C'CCGG_G
Cuts at: 0 18 1089
Size: 18 1071
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BfrBI BglI Bme1580I BpmI BpuEI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BstXI BstYI
BtgI BtsI DrdI EarI EcoICRI Eco57MI EcoRI HaeII
Hin4I HincII MfeI MspA1I NsiI NspI PflMI PshAI
PspOMI PvuII SacI SmaI SmlI StuI TaqII TstI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EaeI EagI EciI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StyI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI ZraI