Restriction map for
pFN21AE1440
(Linear) (Six-base) MAPSORT of: KIEE1440.seq Check: 1561 from: 1 to: 1047
KIEE1440.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AccI GT'mk_AC
Cuts at: 0 56 1047
Size: 56 991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1047
Size: 775 272
AflIII A'CryG_T
Cuts at: 0 1010 1037 1047
Size: 1010 27 10
Fragments arranged by size:
1010 27 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 1047
Size: 298 32 717
Fragments arranged by size:
717 298 32
AlwNI CAG_nnn'CTG
Cuts at: 0 908 1047
Size: 908 139
ApaLI G'TGCA_C
Cuts at: 0 624 1047
Size: 624 423
AvaI C'yCGr_G
Cuts at: 0 506 1047
Size: 506 541
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 760 793 1047
Size: 760 33 254
Fragments arranged by size:
760 254 33
BanI G'GyrC_C
Cuts at: 0 862 1047
Size: 862 185
BanII G_rGCy'C
Cuts at: 0 290 1047
Size: 290 757
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 1047
Size: 267 780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 258 292 465 499 1047
Size: 258 34 173 34 548
Fragments arranged by size:
548 258 173 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 343 1047
Size: 343 704
Bme1580I G_kGCm'C
Cuts at: 0 628 865 1047
Size: 628 237 182
Fragments arranged by size:
628 237 182
BmgBI CAC'GTC
Cuts at: 0 638 1047
Size: 638 409
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 1047
Size: 268 779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1047
Size: 595 452
Bpu10I CC'TnA_GC
Cuts at: 0 382 1047
Size: 382 665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1047
Size: 667 380
BsaAI yAC'GTr
Cuts at: 0 678 711 1013 1040 1047
Size: 678 33 302 27 7
Fragments arranged by size:
678 302 33 27 7
BsaHI Gr'CG_yC
Cuts at: 0 170 1047
Size: 170 877
BsaWI w'CCGG_w
Cuts at: 0 5 62 1047
Size: 5 57 985
Fragments arranged by size:
985 57 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 1047
Size: 298 30 719
Fragments arranged by size:
719 298 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 592 850 1047
Size: 69 523 258 197
Fragments arranged by size:
523 258 197 69
BseYI C'CCAG_C
Cuts at: 0 669 1047
Size: 669 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1035 1047
Size: 239 796 12
Fragments arranged by size:
796 239 12
BsiHKAI G_wGCw'C
Cuts at: 0 290 628 683 1047
Size: 290 338 55 364
Fragments arranged by size:
364 338 290 55
BsmI GAATG_Cn'
Cuts at: 0 528 1047
Size: 528 519
Bsp1286I G_dGCh'C
Cuts at: 0 290 628 683 865 1047
Size: 290 338 55 182 182
Fragments arranged by size:
338 290 182 182 55
BspEI T'CCGG_A
Cuts at: 0 62 1047
Size: 62 985
BspHI T'CATG_A
Cuts at: 0 536 1047
Size: 536 511
BsrDI GCAATG_nn'
Cuts at: 0 240 329 1047
Size: 240 89 718
Fragments arranged by size:
718 240 89
BsrFI r'CCGG_y
Cuts at: 0 161 337 850 1047
Size: 161 176 513 197
Fragments arranged by size:
513 197 176 161
BsrGI T'GTAC_A
Cuts at: 0 806 1034 1047
Size: 806 228 13
Fragments arranged by size:
806 228 13
BssSI C'ACGA_G
Cuts at: 0 621 1047
Size: 621 426
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1013 1047
Size: 1013 34
BstEII G'GTnAC_C
Cuts at: 0 846 1047
Size: 846 201
BstYI r'GATC_y
Cuts at: 0 119 569 1047
Size: 119 450 478
Fragments arranged by size:
478 450 119
BtgI C'CryG_G
Cuts at: 0 606 788 1047
Size: 606 182 259
Fragments arranged by size:
606 259 182
BtsI GCAGTG_nn'
Cuts at: 0 649 861 973 1047
Size: 649 212 112 74
Fragments arranged by size:
649 212 112 74
DraIII CAC_nnn'GTG
Cuts at: 0 612 1047
Size: 612 435
EaeI y'GGCC_r
Cuts at: 0 237 1047
Size: 237 810
EcoICRI GAG'CTC
Cuts at: 0 288 1047
Size: 288 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 775 1047
Size: 595 180 272
Fragments arranged by size:
595 272 180
EcoO109I rG'GnC_Cy
Cuts at: 0 840 1047
Size: 840 207
HaeII r_GCGC'y
Cuts at: 0 21 1047
Size: 21 1026
MslI CAynn'nnrTG
Cuts at: 0 1030 1047
Size: 1030 17
MspA1I CmG'CkG
Cuts at: 0 144 242 361 905 1047
Size: 144 98 119 544 142
Fragments arranged by size:
544 144 142 119 98
NaeI GCC'GGC
Cuts at: 0 163 339 1047
Size: 163 176 708
Fragments arranged by size:
708 176 163
NcoI C'CATG_G
Cuts at: 0 788 1047
Size: 788 259
NgoMIV G'CCGG_C
Cuts at: 0 161 337 1047
Size: 161 176 710
Fragments arranged by size:
710 176 161
PasI CC'CwG_GG
Cuts at: 0 372 867 1047
Size: 372 495 180
Fragments arranged by size:
495 372 180
PfoI T'CCnGG_A
Cuts at: 0 572 1047
Size: 572 475
PmlI CAC'GTG
Cuts at: 0 711 1013 1040 1047
Size: 711 302 27 7
Fragments arranged by size:
711 302 27 7
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 1047
Size: 298 32 717
Fragments arranged by size:
717 298 32
PpuMI rG'GwC_Cy
Cuts at: 0 840 1047
Size: 840 207
PshAI GACnn'nnGTC
Cuts at: 0 215 1047
Size: 215 832
PvuII CAG'CTG
Cuts at: 0 361 1047
Size: 361 686
SacI G_AGCT'C
Cuts at: 0 290 1047
Size: 290 757
SexAI A'CCwGG_T
Cuts at: 0 800 1047
Size: 800 247
SgrAI Cr'CCGG_yG
Cuts at: 0 337 1047
Size: 337 710
SmaI CCC'GGG
Cuts at: 0 508 1047
Size: 508 539
SmlI C'TyrA_G
Cuts at: 0 682 1047
Size: 682 365
StuI AGG'CCT
Cuts at: 0 128 1047
Size: 128 919
StyI C'CwwG_G
Cuts at: 0 488 788 1047
Size: 488 300 259
Fragments arranged by size:
488 300 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 880 958 1047
Size: 880 78 89
Fragments arranged by size:
880 89 78
TatI w'GTAC_w
Cuts at: 0 692 806 1034 1047
Size: 692 114 228 13
Fragments arranged by size:
692 228 114 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 432 464 586 618 1047
Size: 432 32 122 32 429
Fragments arranged by size:
432 429 122 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 1047
Size: 495 552
XmaI C'CCGG_G
Cuts at: 0 506 1047
Size: 506 541
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApaLI AvaI BaeI
BanI BanII BbsI BcgI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI BtsI
DraIII EaeI EcoICRI Eco57MI EcoO109I HaeII MslI MspA1I
NaeI NcoI NgoMIV PasI PfoI PmlI PpiI PpuMI
PshAI PvuII SacI SexAI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BciVI BclI BfrBI BglII BlpI
BmtI BplI BsaI BsaBI BsiEI BsiWI BsmBI BspMI
BsrBI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI