(Linear) (Six-base) MAPSORT of: KIEE1439.seq  Check: 1840  from: 1  to: 387



KIEE1439.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     156     387 
   Size:        156     231
 
AlwNI CAG_nnn'CTG

Cuts at:      0     122     387 
   Size:        122     265
 
AvaI C'yCGr_G

Cuts at:      0       5     387 
   Size:          5     382
 
BanI G'GyrC_C

Cuts at:      0      94     387 
   Size:         94     293
 
BanII G_rGCy'C

Cuts at:      0      13      50     387 
   Size:         13      37     337

  Fragments arranged by size: 

                 337      37      13
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     321     387 
   Size:        144     177      66

  Fragments arranged by size: 

                 177     144      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     340     387 
   Size:        340      47
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     358     387 
   Size:         25     333      29

  Fragments arranged by size: 

                 333      29      25
 
BseYI C'CCAG_C

Cuts at:      0      42     273     366     387 
   Size:         42     231      93      21

  Fragments arranged by size: 

                 231      93      42      21
 
BsiEI CG_ry'CG

Cuts at:      0     175     387 
   Size:        175     212
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     297     387 
   Size:        297      90
 
Bsp1286I G_dGCh'C

Cuts at:      0      13      50     387 
   Size:         13      37     337

  Fragments arranged by size: 

                 337      37      13
 
BsrFI r'CCGG_y

Cuts at:      0      20     349     387 
   Size:         20     329      38

  Fragments arranged by size: 

                 329      38      20
 
BssSI C'ACGA_G

Cuts at:      0      37     387 
   Size:         37     350
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     152     214     387 
   Size:        152      62     173

  Fragments arranged by size: 

                 173     152      62
 
BtgI C'CryG_G

Cuts at:      0      71     111     387 
   Size:         71      40     276

  Fragments arranged by size: 

                 276      71      40
 
EaeI y'GGCC_r

Cuts at:      0      74     387 
   Size:         74     313
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     368     387 
   Size:        368      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     321     387 
   Size:        144     177      66

  Fragments arranged by size: 

                 177     144      66
 
HaeII r_GCGC'y

Cuts at:      0      91     254     379     387 
   Size:         91     163     125       8

  Fragments arranged by size: 

                 163     125      91       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     387 
   Size:        142      32     213

  Fragments arranged by size: 

                 213     142      32
 
MspA1I CmG'CkG

Cuts at:      0     113     370     387 
   Size:        113     257      17

  Fragments arranged by size: 

                 257     113      17
 
PfoI T'CCnGG_A

Cuts at:      0     121     387 
   Size:        121     266
 
PstI C_TGCA'G

Cuts at:      0     207     387 
   Size:        207     180
 
PvuII CAG'CTG

Cuts at:      0     370     387 
   Size:        370      17
 
SacII CC_GC'GG

Cuts at:      0     114     387 
   Size:        114     273
 
SfcI C'TryA_G

Cuts at:      0     203     387 
   Size:        203     184
 
StyI C'CwwG_G

Cuts at:      0     243     387 
   Size:        243     144
 

 Enzymes that do cut:

     AhdI    AlwNI     AvaI     BanI    BanII     BpmI     BsaI    BseRI
    BseYI    BsiEI    BsmBI Bsp1286I    BsrFI    BssSI   BstAPI     BtgI
     EaeI     EciI  Eco57MI    HaeII    Hin4I   MspA1I     PfoI     PstI
    PvuII    SacII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI