Restriction map for
pFN21AE1437
(Linear) (Six-base) MAPSORT of: KIEE1437.seq Check: 2962 from: 1 to: 3033
KIEE1437.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1096 3033
Size: 1096 1937
AccI GT'mk_AC
Cuts at: 0 1343 1492 3027 3033
Size: 1343 149 1535 6
Fragments arranged by size:
1535 1343 149 6
Acc65I G'GTAC_C
Cuts at: 0 1171 3033
Size: 1171 1862
AclI AA'CG_TT
Cuts at: 0 2615 3033
Size: 2615 418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 3033
Size: 581 2452
AflII C'TTAA_G
Cuts at: 0 1335 3033
Size: 1335 1698
AflIII A'CryG_T
Cuts at: 0 1787 3033
Size: 1787 1246
AhdI GACnn_n'nnGTC
Cuts at: 0 287 3033
Size: 287 2746
AleI CACnn'nnGTG
Cuts at: 0 2071 2640 3033
Size: 2071 569 393
Fragments arranged by size:
2071 569 393
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 418 902 3033
Size: 418 484 2131
Fragments arranged by size:
2131 484 418
AlwNI CAG_nnn'CTG
Cuts at: 0 1582 3033
Size: 1582 1451
ApaI G_GGCC'C
Cuts at: 0 970 3033
Size: 970 2063
ApoI r'AATT_y
Cuts at: 0 136 623 1520 2465 2536 2580 3033
Size: 136 487 897 945 71 44 453
Fragments arranged by size:
945 897 487 453 136 71 44
AseI AT'TA_AT
Cuts at: 0 2207 3033
Size: 2207 826
AvaI C'yCGr_G
Cuts at: 0 647 3033
Size: 647 2386
BamHI G'GATC_C
Cuts at: 0 1364 3033
Size: 1364 1669
BanI G'GyrC_C
Cuts at: 0 1171 1914 2055 3033
Size: 1171 743 141 978
Fragments arranged by size:
1171 978 743 141
BanII G_rGCy'C
Cuts at: 0 505 935 970 1974 2113 2317 3033
Size: 505 430 35 1004 139 204 716
Fragments arranged by size:
1004 716 505 430 204 139 35
BbvCI CC'TCA_GC
Cuts at: 0 1481 3033
Size: 1481 1552
BclI T'GATC_A
Cuts at: 0 2126 3033
Size: 2126 907
BglII A'GATC_T
Cuts at: 0 2489 3033
Size: 2489 544
BlpI GC'TnA_GC
Cuts at: 0 2509 2741 3033
Size: 2509 232 292
Fragments arranged by size:
2509 292 232
Bme1580I G_kGCm'C
Cuts at: 0 970 3033
Size: 970 2063
BmgBI CAC'GTC
Cuts at: 0 1377 1788 3033
Size: 1377 411 1245
Fragments arranged by size:
1377 1245 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 919 1450 1825 2401 3033
Size: 91 828 531 375 576 632
Fragments arranged by size:
828 632 576 531 375 91
Bpu10I CC'TnA_GC
Cuts at: 0 1481 3033
Size: 1481 1552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1573 1840 2906 3033
Size: 752 821 267 1066 127
Fragments arranged by size:
1066 821 752 267 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 1760 3033
Size: 274 1486 1273
Fragments arranged by size:
1486 1273 274
BsaAI yAC'GTr
Cuts at: 0 819 3033
Size: 819 2214
BsaHI Gr'CG_yC
Cuts at: 0 974 2214 3033
Size: 974 1240 819
Fragments arranged by size:
1240 974 819
BsaWI w'CCGG_w
Cuts at: 0 1361 2146 2948 3033
Size: 1361 785 802 85
Fragments arranged by size:
1361 802 785 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1130 1160 2144 2174 3033
Size: 1130 30 984 30 859
Fragments arranged by size:
1130 984 859 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 467 919 922 943 1060 1822 2165
Size: 21 446 452 3 21 117 762 343
Cuts at: 2165 2466 2744 3033
Size: 301 278 289
Fragments arranged by size:
762 452 446 343 301 289 278 117
21 21 3
BseYI C'CCAG_C
Cuts at: 0 1440 1631 2106 3033
Size: 1440 191 475 927
Fragments arranged by size:
1440 927 475 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1221 2111 3033
Size: 1221 890 922
Fragments arranged by size:
1221 922 890
BsiHKAI G_wGCw'C
Cuts at: 0 654 833 881 935 2168 2317 3033
Size: 654 179 48 54 1233 149 716
Fragments arranged by size:
1233 716 654 179 149 54 48
Bsp1286I G_dGCh'C
Cuts at: 0 505 654 833 881 935 970 1974 2113
Size: 505 149 179 48 54 35 1004 139
Cuts at: 2113 2168 2317 3033
Size: 55 149 716
Fragments arranged by size:
1004 716 505 179 149 149 139 55
54 48 35
BspEI T'CCGG_A
Cuts at: 0 2146 2948 3033
Size: 2146 802 85
Fragments arranged by size:
2146 802 85
BspHI T'CATG_A
Cuts at: 0 2802 3033
Size: 2802 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1096 3033
Size: 1096 1937
BssSI C'ACGA_G
Cuts at: 0 880 1292 3033
Size: 880 412 1741
Fragments arranged by size:
1741 880 412
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2601 3033
Size: 2601 432
BstYI r'GATC_y
Cuts at: 0 605 1364 2489 3033
Size: 605 759 1125 544
Fragments arranged by size:
1125 759 605 544
BstZ17I GTA'TAC
Cuts at: 0 1493 3033
Size: 1493 1540
Bsu36I CC'TnA_GG
Cuts at: 0 1369 2449 3033
Size: 1369 1080 584
Fragments arranged by size:
1369 1080 584
BtgI C'CryG_G
Cuts at: 0 1992 3033
Size: 1992 1041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1976 3033
Size: 1976 1057
BtsI GCAGTG_nn'
Cuts at: 0 402 3033
Size: 402 2631
ClaI AT'CG_AT
Cuts at: 0 861 3033
Size: 861 2172
DraI TTT'AAA
Cuts at: 0 1565 1589 2418 3033
Size: 1565 24 829 615
Fragments arranged by size:
1565 829 615 24
DraIII CAC_nnn'GTG
Cuts at: 0 751 3033
Size: 751 2282
DrdI GACnn_nn'nnGTC
Cuts at: 0 1417 3033
Size: 1417 1616
EaeI y'GGCC_r
Cuts at: 0 1008 2063 2870 3033
Size: 1008 1055 807 163
Fragments arranged by size:
1055 1008 807 163
EarI CTCTTCn'nnn_
Cuts at: 0 569 2365 3033
Size: 569 1796 668
Fragments arranged by size:
1796 668 569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1723 3033
Size: 1723 1310
EcoICRI GAG'CTC
Cuts at: 0 933 2315 3033
Size: 933 1382 718
Fragments arranged by size:
1382 933 718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 581 919 1450 1825 2401 3033
Size: 91 490 338 531 375 576 632
Fragments arranged by size:
632 576 531 490 375 338 91
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1676 1777 3033
Size: 1676 101 1256
Fragments arranged by size:
1676 1256 101
EcoO109I rG'GnC_Cy
Cuts at: 0 591 967 1437 2121 2720 3033
Size: 591 376 470 684 599 313
Fragments arranged by size:
684 599 591 470 376 313
EcoRI G'AATT_C
Cuts at: 0 2580 3033
Size: 2580 453
FspI TGC'GCA
Cuts at: 0 11 2854 3033
Size: 11 2843 179
Fragments arranged by size:
2843 179 11
FspAI rTGC'GCAy
Cuts at: 0 2854 3033
Size: 2854 179
HaeII r_GCGC'y
Cuts at: 0 1983 3033
Size: 1983 1050
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 1774 1806 2784 2816 3033
Size: 273 32 1469 32 978 32 217
Fragments arranged by size:
1469 978 273 217 32 32 32
HincII GTy'rAC
Cuts at: 0 3028 3033
Size: 3028 5
HindIII A'AGCT_T
Cuts at: 0 125 3033
Size: 125 2908
KpnI G_GTAC'C
Cuts at: 0 1175 3033
Size: 1175 1858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 2265 3033
Size: 1856 409 768
Fragments arranged by size:
1856 768 409
MscI TGG'CCA
Cuts at: 0 1010 2065 3033
Size: 1010 1055 968
Fragments arranged by size:
1055 1010 968
MslI CAynn'nnrTG
Cuts at: 0 296 884 985 2053 2071 2599 2640 2942
Size: 296 588 101 1068 18 528 41 302
Cuts at: 2942 3033
Size: 91
Fragments arranged by size:
1068 588 528 302 296 101 91 41
18
MspA1I CmG'CkG
Cuts at: 0 1994 3033
Size: 1994 1039
NspI r_CATG'y
Cuts at: 0 783 3033
Size: 783 2250
PasI CC'CwG_GG
Cuts at: 0 318 3033
Size: 318 2715
PflMI CCAn_nnn'nTGG
Cuts at: 0 2284 3033
Size: 2284 749
PfoI T'CCnGG_A
Cuts at: 0 760 935 1802 3033
Size: 760 175 867 1231
Fragments arranged by size:
1231 867 760 175
PmlI CAC'GTG
Cuts at: 0 819 3033
Size: 819 2214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2741 2773 3033
Size: 2741 32 260
Fragments arranged by size:
2741 260 32
PpuMI rG'GwC_Cy
Cuts at: 0 591 1437 3033
Size: 591 846 1596
Fragments arranged by size:
1596 846 591
PspOMI G'GGCC_C
Cuts at: 0 966 3033
Size: 966 2067
SacI G_AGCT'C
Cuts at: 0 935 2317 3033
Size: 935 1382 716
Fragments arranged by size:
1382 935 716
SacII CC_GC'GG
Cuts at: 0 1995 3033
Size: 1995 1038
SalI G'TCGA_C
Cuts at: 0 3026 3033
Size: 3026 7
SfcI C'TryA_G
Cuts at: 0 1744 2724 2889 3033
Size: 1744 980 165 144
Fragments arranged by size:
1744 980 165 144
SmlI C'TyrA_G
Cuts at: 0 647 767 1335 1552 1855 2921 3033
Size: 647 120 568 217 303 1066 112
Fragments arranged by size:
1066 647 568 303 217 120 112
SpeI A'CTAG_T
Cuts at: 0 1275 3033
Size: 1275 1758
SspI AAT'ATT
Cuts at: 0 850 3033
Size: 850 2183
StyI C'CwwG_G
Cuts at: 0 587 3033
Size: 587 2446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 158 3033
Size: 158 2875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1130 1162 2144 2176 3033
Size: 1130 32 982 32 857
Fragments arranged by size:
1130 982 857 32 32
XbaI T'CTAG_A
Cuts at: 0 1536 3033
Size: 1536 1497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 223 315 2433 3033
Size: 87 136 92 2118 600
Fragments arranged by size:
2118 600 136 92 87
XhoI C'TCGA_G
Cuts at: 0 647 3033
Size: 647 2386
XmnI GAAnn'nnTTC
Cuts at: 0 424 3033
Size: 424 2609
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApoI AseI AvaI BamHI
BanI BanII BbvCI BclI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI SacI
SacII SalI SfcI SmlI SpeI SspI StyI TaqII
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AloI ApaLI AscI AsiSI AvrII
BaeI BbeI BbsI BcgI BciVI BfrBI BglI BmrI
BmtI BplI BsaBI BsiEI BsiWI BsmI BsmBI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII CspCI
EagI EcoRV FalI FseI HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI StuI SwaI TatI Tth111I XmaI ZraI