(Linear) (Six-base) MAPSORT of: KIEE1432.seq  Check: 6165  from: 1  to: 600



KIEE1432.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     600 
   Size:        490     110
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     413     600 
   Size:        413     187
 
ApoI r'AATT_y

Cuts at:      0     184     325     600 
   Size:        184     141     275

  Fragments arranged by size: 

                 275     184     141
 
BanI G'GyrC_C

Cuts at:      0     205     600 
   Size:        205     395
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     478     600 
   Size:        478     122
 
Bpu10I CC'TnA_GC

Cuts at:      0     147     600 
   Size:        147     453
 
BsaWI w'CCGG_w

Cuts at:      0     315     416     600 
   Size:        315     101     184

  Fragments arranged by size: 

                 315     184     101
 
BseYI C'CCAG_C

Cuts at:      0     284     600 
   Size:        284     316
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     600 
   Size:        200     400
 
BspEI T'CCGG_A

Cuts at:      0     315     416     600 
   Size:        315     101     184

  Fragments arranged by size: 

                 315     184     101
 
BsrDI GCAATG_nn'

Cuts at:      0     495     600 
   Size:        495     105
 
BstBI TT'CG_AA

Cuts at:      0     189     600 
   Size:        189     411
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     114     292     600 
   Size:        114     178     308

  Fragments arranged by size: 

                 308     178     114
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     552     587     600 
   Size:        552      35      13

  Fragments arranged by size: 

                 552      35      13
 
EaeI y'GGCC_r

Cuts at:      0     116     600 
   Size:        116     484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     600 
   Size:        490     110
 
HindIII A'AGCT_T

Cuts at:      0     456     600 
   Size:        456     144
 
MscI TGG'CCA

Cuts at:      0     118     600 
   Size:        118     482
 
MslI CAynn'nnrTG

Cuts at:      0     395     600 
   Size:        395     205
 
NspI r_CATG'y

Cuts at:      0     400     600 
   Size:        400     200
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      21      53     600 
   Size:         21      32     547

  Fragments arranged by size: 

                 547      32      21
 
ScaI AGT'ACT

Cuts at:      0      60     600 
   Size:         60     540
 
SphI G_CATG'C

Cuts at:      0     400     600 
   Size:        400     200
 
StuI AGG'CCT

Cuts at:      0     242     600 
   Size:        242     358
 
StyI C'CwwG_G

Cuts at:      0      87     600 
   Size:         87     513
 
TatI w'GTAC_w

Cuts at:      0      58     600 
   Size:         58     542
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BanI     BbsI   Bpu10I    BsaWI    BseYI
     BsgI    BspEI    BsrDI    BstBI    BstXI    CspCI     EaeI  Eco57MI
  HindIII     MscI     MslI     NspI     PsrI     ScaI     SphI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI